Chinese Journal of Rice Science

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Development of STS Marker Linked to Rice Blast Resistance Gene Pi9 in Marker Assisted Selection Breeding

YIN De-suo; XIA Ming-yuan; LI Jin-bo;WAN Bing-liang;ZHA Zhong-ping;DU Xue-shu; QI Hua-xiong*   

  1. Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; *Corresponding author, E-mail: qihx@hbaas.com
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-10 Published:2011-01-10

抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用

殷得所;夏明元;李进波;万丙良;査中萍;杜雪树;戚华雄*   

  1. 湖北省农业科学院 粮食作物研究所, 湖北 武汉 430064; *通讯联系人, E-mail: qihx@hbaas.com

Abstract:

Indica rice line 751127 carrying broadspectrum rice blast resistance gene Pi9 was used as resistance gene donor for genetic improvement of Yangdao 6 and R6547. By alignment of genomic sequences from the Pi9 locus of 751127, Yangdao 6 and Nipponbare, codominant STS (sequencetagged site) marker PB91, which linked to the Pi9 gene, was developed for molecular markerassisted selection (MAS). Eight backcross lines carrying Pi9 were obtained, among which four lines contained the genetic background of Yangdao 6 and the other four lines had the R6547 background. Under natural disease infection conditions in Enshi County and Yichang County of Hubei Province, China, the Pi9carrying lines showed higher blast disease resistance than Yangdao 6 and R6547 in various degrees. The hybrids originated from 08C893, an R6547derived rice line, showed higher blast disease resistance as compared to the control Yangliangyou 6. The results suggest PB91 is an effective codominant marker for MAS of Pi9 which is a valuable resistance gene for the control of rice blast disease in Hubei area.

Key words: blast resistance, molecular markerassisted selection, sequencetagged site, resistance gene, breading, rice

摘要: 利用带有广谱抗稻瘟病基因Pi9的籼稻品系751127作为抗病基因供体亲本,用于扬稻6号和R6547抗病性的回交育种。通过比较751127、扬稻6号和日本晴的Pi9基因位点的DNA序列,开发出了与Pi9基因紧密连锁的共显性STS(序列标记位点)标记PB91,用于Pi9基因的分子标记辅助选择。结合田间农艺性状选择和分子标记辅助选择,培育出8个Pi9基因纯合的回交后代株系。其中,具有扬稻6号和R6547遗传背景的株系各4个。经湖北恩施和宜昌的病圃鉴定,携带有抗病基因株系的稻瘟病抗性水平较受体品种扬稻6号和R6547有不同程度的提高。具有R6547遗传背景的株系08C893配制的杂交组合在上述病区的抗性表现也明显优于对照品种扬两优6号。上述结果说明,共显性标记PB91在Pi9抗稻瘟病基因分子标记辅助育种中具有应用价值,并且Pi9基因作为稻瘟病抗源之一可以在湖北稻区进行有效利用。

关键词: 稻瘟病抗性, 分子标记辅助选择, 序列标记位点, 抗性基因, 育种, 水稻