中国水稻科学
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  2011, Vol. 25 Issue (1): 19-24     DOI: 10.3969/j.issn.1001-7216.2011.01.003
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Construction of Molecular Fingerprinting Database and Dual PCR Assay for Two Indica Rice Hybrids and Two Japonica Rice Varieties by Using SSR Markers
CHEN Shu-lin1; WANG Hai-yan1,*;DING Zhen-qian2;WANG Xiu-e1;CHEN Zhong-ming2,*

1State Key Laboratory of Crop Genetics and Germplasm Enhancement/Institute of Cytogenetics, Nanjing Agricultural University, Nanjing 210095, China; 2Jiangsu Mingtian Seeds Science and Technology Co. Ltd/Jiangsu Crop Seeds Engineering Research Center, Nanjing 210014, China; *Corresponding authors, E-mail: hywang@njau.edu.cn; zhongmingchen136@sina.com

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Abstract A total of 460 SSR primers were used for amplification of five indica hybrid rice combinations and their parents, and 16 japonica rice varieties (or elite lines). By using the identified codominant specific primers, molecular fingerprinting databases for Guodao 6, Liangyoupeijiu,Yandao 8 and Xudao 4 were constructed. A total of 5, 3, 1 and 2 SSR primers were identified to be specific for Guodao 6, Liangyoupeijiu, Yandao 8 and Xudao 4, respectively. These fingerprints can be used to examine the seed purity of these rice varieties. In addition, to develop dual PCR assay in examination of seed purity and authenticity, SSR primers which could stably amplify clear bands and have good specificity were selected and used in multiplex popymerase chain reaction amplification.
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CHEN Shu-lin
WANG Hai-yan
DING Zhen-qian
WANG Xiu-e
CHEN Zhong-ming
Key wordshybrid rice   seed purity   seed authenticity   fingerprinting   dual polymerase chain reaction assay   seed identification     
Received: 1900-01-01;
Cite this article:   
CHEN Shu-lin,WANG Hai-yan,DING Zhen-qian et al. Construction of Molecular Fingerprinting Database and Dual PCR Assay for Two Indica Rice Hybrids and Two Japonica Rice Varieties by Using SSR Markers
[J]. , 2011, 25(1): 19-24 .
 
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