Chinese Journal of Rice Science

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Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Line GD8S by Molecular Marker Assisted Selection

JIN Su-juan , LIU Wu-ge , ZHU Xiao-yuan , WANG Feng , LI Jin-hua , LIU Zhen-rong , LIAO Yi-long , ZHU Man-shan , HUANG Hui-jun , LIU Yi-bai   

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-11-10 Published:2007-11-10
  • Contact: WANG Feng

利用分子标记辅助选择改良温敏核不育系GD-8S的稻瘟病抗性

金素娟1, 2, #;柳武革1, #;朱小源3;王丰1, *; 李金华1;刘振荣1;廖亦龙1;朱满山1;黄慧君1;刘宜柏2   

  1. 1广东省农业科学院 水稻研究所, 广东 广州 510640; 2江西农业大学 农学院, 江西 南昌 330045; 3广东省农业科学院 植物保护研究所, 广东 广州 510640;

Abstract: The broadspectrum blast resistance gene Pi1, from the donor line BL122, was introduced into a thermosensitive genic male sterile (TGMS) line GD8S, which possessed good grain quality but high susceptibility to rice blast, by using backcross breeding and molecular markerassisted selection. Five elite improved male sterile lines RGD8S1, RGD8S2, RGD8S3, RGD8S4 and RGD8S5, were selected based on the results of molecular marker analysis, spikelet sterility, recovery ratio of genetic background and agronomic traits. Thirtythree representative blast isolates collected from Guangdong Province, China were used to inoculate the improved lines and the origin line GD8S artificially. The resistant frequency of the improved lines ranged from 75.76% to 100.00%, much higher than that of the check GD8S, which was only 9.09%. On the agronomic characters, there was no significant differences between the improved lines and the check GD8S except for flag leaf length and panicle number per plant. The improved lines could be used for breeding hybrid rice with high blast resistance.

Key words: blast resistance, thermo-sensitive genic male sterile line, molecular markerassisted selection, breeding, rice

摘要: 以含有广谱稻瘟病抗性基因Pi1的籼稻材料BL122为供体,优质感病温敏核不育系GD8S为受体,通过杂交、多次回交和自交结合分子标记辅助选择技术,将Pi1基因导入GD8S中。综合目标基因的分子检测、育性镜检、田间农艺性状表现以及遗传背景的回复率,筛选获得5个改良株系RGD8S1、RGD8S2、RGD8S3、RGD8S4和RGD8S5。采用广东省具有代表性的33个菌株进行人工接种抗性鉴定,5个改良株系的抗性频率为75.76%~100.00%,而对照仅为9.09%。农艺性状分析表明,除剑叶长和每株穗数外,其余性状改良株系与对照无显著差异。这些改良株系可用于进一步测配选育高抗稻瘟病杂交稻新组合。

关键词: 稻瘟病抗性, 温敏核不育系, 分子标记辅助选择, 育种, 水稻