Chinese Journal of Rice Science

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Identification of a Herbicide Safener AD-67 Inducible cDNA in Rice

YIN De-suo , SUN Xiao-qiong , LI Ke , WANG Shi-quan , DENG Qi-ming , LI Ping
  

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-07-10 Published:2007-07-10

一个除草剂安全剂AD-67诱导的水稻基因片段的克隆和分析

殷得所1,2;孙小琼3;李科1,2 ;王世全1,2; 邓其明1,2;李 平1,2,*   

  1. 1四川农业大学 水稻研究所, 四川 成都 611130; Email: ydsscnd@hotmail.com; 2四川农业大学 西南作物基因资源与遗传改良教育部重点实验室, 四川 雅安 625014; 3四川农大高科农业有限责任公司, 四川 成都 611130; *通讯联系人, E-mail:liping@cngk.com

Abstract: A safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display.The transcript increased 6 h after the rice seedlings were sprayed with the safener solution and 5 days later,the expression could still be detected.The fragment was recycled from the poly-gel and sequenced,and homologous analysis revealed the cDNA was 100% identical to some ESTs and cDNAs in rice data base,and showed 60%-84% homologous to the gene Yippee in several eukaryotes in amino acid sequence.The fragment was extended to a 831 bp cDNA,a primer was designed and RT-PCR analysis supported the induction result.

Key words: rice, chemical induction, mRNA differential display, S-ethyl-N, N-dipropylthiocarbonate, Yippee gene

摘要: 为寻找化学诱导表达基因,利用mRNA差异显示方法,从除草剂安全剂AD67(苯叉酰胺)诱导处理的水稻品种9311的幼苗中筛选分离得到1个诱导表达增强的cDNA片段。该片段在处理后6 h表达明显增强,直到第5天仍然有表达。从polygel上回收片段并测序,核酸同源序列分析显示它与水稻多个EST和cDNA序列一致性达到100%,氨基酸序列分析表明它与水稻、四膜虫、隐球酵母等真核生物的Yippee基因相似性达60%~84%。序列经延伸得到831 bp的cDNA,由此设计引物并进行RTPCR分析,结果说明该基因在除草剂安全剂AD67诱导后表达增强。

关键词: 水稻, 化学诱导, 差异显示, 苯叉酰胺, Yippee基因