Abstract: Three standard indica test varieties,three standard japonica test varieties and other thirteen rice accessions,which are Yongyou series hybrid rice combinations and their parents,were analyzed by using 22 simple sequence repeat(SSR)primer pairs.Totally 87 alleles were detected.These primers revealed polymorphism with a range of 2-8 alleles per primer pair.Fingerprintings of Yongyou series hybrid rice combinations and their parents were set up.The cluster analysis showed that the tested materials were grouped into two clusters in accordance with the indica/japonica classification based on the Cheng’s index.It was confirmed that Yongyou 4 and Yongyou 6 are inter-subspecific hybrid rice combinations between indica and japonica at molecular levels.

Key words: hybrid rice, simple sequence repeat(SSR), fingerprinting, indica/japonica classification

摘要： 用22个SSR分子标记对13份甬优系列杂交水稻组合及其亲本材料和6份籼粳对照种进行分析，共获得87个等位基因，每对引物扩增的等位基因数为2~8个。所有供试材料间均检测到了差异，从而建立起甬优1号~甬优6号杂交水稻组合及其亲本的DNA指纹图谱。聚类分析结果表明，9份供试材料与3份粳稻对照种聚于一类，属粳稻；4份供试材料与籼稻对照种IR36聚于一类，属籼稻。这与形态分类结果基本一致。从分子水平确认了杂交水稻甬优6号和甬优4号为籼粳亚种间杂交稻。

关键词: 杂交水稻, 微卫星标记, 指纹图谱, 籼粳分类