Improve the Character of Rice Plants Utilizing Potassium by Overexpressed Foreign Potassium Channel Genes

• 研究简报 • Previous Articles Next Articles

Improve the Character of Rice Plants Utilizing Potassium by Overexpressed Foreign Potassium Channel Genes

YAN Wei-dong ¹; WANG Xiao-chang¹; HE Si-jie ²; TANG Li ¹; AN Zhi-zhuang ¹; TIAN Wen-zhong ²; SHI Wei-ming^{1; *}; CAO Zhi-hong¹

(¹ Institute of Soil Science; Chinese Academy of Sciences; Nanjing 210008; China; ²Institute of Genetics; Chinese Academy of Sciences, Beijing 100101, China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-01-10 Published:2002-01-10

利用外源钾通道基因改良水稻钾素营养

严蔚东 1; 王校常 1; 何锶洁 2; 汤利 1; 安志装 1; 田文忠 2; 施卫明 1,*; 曹志洪 1

1中国科学院南京土壤研究所，江苏南京 210008；2 中国科学院遗传研究所，北京 100101；*通讯联系人，E-mail:wmshi@issas.ac.cn

Abstract

Abstract: The potassium channel genes (KAT1 and AKT1) were introduced into the rice (Zhonghua 8, Zhonghua 9, Zhonghua 13 and 8706) by using particle bombardment method. The molecular analysis confirmed the integration and expression of these genes in the transgenic rice plants. The results showed that transgenic plants have the high capacity of accumulating potassium in a pot cultural experiment with two different K levels.

Key words: potassium channel gene, rice, potassium, plant nutrition, gene transfer

摘要： 通过基因枪导入法，将外源钾通道基因 (KAT1和AKT1)导入水稻中花8号、中花9号、中花13以及8706中，获得了转基因植株，经分子检测，证实了钾通道基因在水稻基因组中的整合与表达。盆栽试验表明，转基因植株在低钾和高钾两种钾水平下其钾的累积能力都有明显提高。

关键词: 钾通道基因, 水稻, 钾素, 植物营养

YAN Wei-dong ,WANG Xiao-chang ,HE Si-jie ,TANG Li ,AN Zhi-zhuang ,TIAN Wen-zhong ,SHI Wei-ming ,CAO Zhi-hong . Improve the Character of Rice Plants Utilizing Potassium by Overexpressed Foreign Potassium Channel Genes[J]. Chinese Journal of Rice Science.

严蔚东 , 王校常 , 何锶洁 , 汤利 , 安志装 , 田文忠 , 施卫明 , 曹志洪 . 利用外源钾通道基因改良水稻钾素营养[J]. 中国水稻科学.

[1]	REN Zhiqi, XUE Kexin, DONG Zheng, LI Xiaoxiang, LI Yongzhao, GUO Yujing, LIU Wenqiang, GUO Liang, SHENG Xinnian, LIU Zhixi, PAN Xiaowu. Identification and Gene Mapping of Outcurved Leaf Mutant ocl1 in Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 337-346.
[2]	XIAO Lequan, LI Lei, DAI Weimin, QIANG Sheng, SONG Xiaoling. Seedling Growth Characteristics of Hybrids Between Transgenic Rice with cry2A/bar* Genes and Weedy Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 347-358.
[3]	LI Gang, GAO Qingsong, LI Wei, ZHANG Wenxia, WANG Jian, CHEN Baoshan, WANG Di, GAO Hao, XU Weijun, CHEN Hongqi, JI Jianhui. Directed Knockout of SD1 Gene Improves Lodging Resistance and Blast Resistance of Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 359-367.
[4]	WANG Shengyong, CHEN Yuhang, CHEN Huili, HUANG Yujie, ZHANG Xiaotian, DING Shuangcheng, WANG Hongwei. Effects of High Temperature on Phenylpropane Metabolism and Downstream Branch Metabolic Pathways in Rice Meiosis [J]. Chinese Journal OF Rice Science, 2023, 37(4): 368-378.
[5]	HUANG Yaru, XU Peng, WANG Lele, HE Yizhe, WANG Hui, KE Jian, HE Haibing, WU Liquan, YOU Cuicui. Effects of Exogenous Trehalose on Grain Filling Characteristics and Yield Formation of japonica Rice Cultivar W1844 [J]. Chinese Journal OF Rice Science, 2023, 37(4): 379-391.
[6]	DONG Liqiang, YANG Tiexin, LI Rui, SHANG Wenqi, MA Liang, LI Yuedong, SUI Guomin. Effect of Plant-row Spacing on Rice Yield and Root Morphological and Physiological Characteristics in Super High Yield Field [J]. Chinese Journal OF Rice Science, 2023, 37(4): 392-404.
[7]	GAO Qianqing, REN Xiaojian, ZHAI Zhongbing, ZHENG Pubing, WU Yuanfen, CUI Kehui. Effects of Panicle and Bud-promoting Nitrogen Fertilizer Application on Growth of Regenerated Bud and Grain Yield of Ratoon Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 405-414.
[8]	HUANG Jinwen, LI Rikun, CHEN Zhicheng, ZHANG Bianhong, LEI Han, PAN Ruixin, YANG Mingyu, PAN Meiqing, TANG Lina. Effects of Straw Returning Techniques on Soil Nutrients, Organic Carbon and Microbial Diversity in Tobacco-rice Rotation System [J]. Chinese Journal OF Rice Science, 2023, 37(4): 415-426.
[9]	HAN Cong, HE Yuchang, WU Lijuan, JIA Lili, WANG Lei, E Zhiguo. Research Progress in the Function of Basic Leucine Zipper (bZIP) Protein Family in Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 436-448.
[10]	SHEN Yumin, CHEN Mingliang, XIONG Huanjin, XIONG Wentao, WU Xiaoyan, XIAO Yeqing. Phenotypic Analysis and Fine Mapping of blg1(beak like grain 1), a Rice Mutant with Abnormal Palea and Lemma Development [J]. Chinese Journal OF Rice Science, 2023, 37(3): 225-232.
[11]	DUAN Min, XIE Liujie, GAO Xiuying, TANG Haijuan, HUANG Shanjun, PAN Xiaobiao. Creation of Thermo-sensitive Genic Male Sterile Rice Lines with Wide Compatibility Based on CRISPR/Cas9 Technology [J]. Chinese Journal OF Rice Science, 2023, 37(3): 233-243.
[12]	WANG Wenting, MA Jiaying, LI Guangyan, FU Weimeng, LI Hubo, LIN Jie, CHEN Tingting, FENG Baohua, TAO Longxing, FU Guanfu, QIN Yebo. Effect of Different Fertilizer Application Rates on Rice Yield and Quality Formation and Its Relationship with Energy Metabolism at High Temperature [J]. Chinese Journal OF Rice Science, 2023, 37(3): 253-264.
[13]	LIU Aihua, LI Xiaokun. Meta-analysis of Relationship Between Fertilizer Application and Rice Quality [J]. Chinese Journal OF Rice Science, 2023, 37(3): 276-284.
[14]	YANG Xiaolong, WANG Biao, WANG Benfu, ZHANG Zhisheng, ZHANG Zuolin, YANG Lantian, CHENG Jianping, LI Yang. Effects of Different Water Management on Yield and Rice Quality of Dry-seeded Rice [J]. Chinese Journal OF Rice Science, 2023, 37(3): 285-294.
[15]	WEI Xiaodong, SONG Xuemei, ZHAO Ling, ZHAO Qingyong, CHEN Tao, LU Kai, ZHU Zhen, HUANG Shengdong, WANG Cailin, ZHANG Yadong. Effects of Silicon and Zinc Fertilizer and Their Application Ways on Yield and Grain Quality of Rice Variety Nanjing 46 [J]. Chinese Journal OF Rice Science, 2023, 37(3): 295-306.