Mapping of a New Rice Blast Resistance Gene in Ziyu 44, a Rice Landrace from Yunnan Province, China

doi:10.16819/j.1001-7216.2019.8054

Abstract

Abstract:

【Objective】Ziyu 44 (Oryza sativa L. subsp. geng), a landrace of Yunnan Province, has broad-spectrum and durable blast resistance. In order to understand the molecular mechanism of resistance to rice blast, 【Method】we carried out the genetic analysis of resistance of Ziyu 44 to the Magnaporthe oryzea LP36 from Luoping County, Yunnan Province, and mapped the gene responsible for resistance to strain LP36 by using an F₂ population from the cross between Jiangnanxiangnuo and Ziyu 44. 【Result】The resistance of Ziyu 44 to LP36 is controlled by a single dominant gene, termed as Pi-zy4(t), which was located on the long arm of chromosome 4 between simple sequence repeat markers RM5503 and RM3276 within about 318 kb.【Conclusion】Pi-zy4(t) is a new gene conferring resistance to rice blast. Our results provide essential information for further utilization of the blast resistance gene in rice breeding and its cloning.

Key words: rice, rice blast, blast resistance gene, gene mapping

摘要：

【目的】子预44是具有广谱持久稻瘟病抗性的云南地方粳稻品种。为了揭示子预44广谱持久抗瘟机制并在抗稻瘟病育种中进行有效利用,【方法】利用江南香糯与子预44杂交构建的遗传群体及分离自云南罗平的稻瘟病菌株LP36进行子预44的抗性遗传分析和抗性基因定位。【结果】子预44对LP36的抗性为单显性基因控制,暂定名为Pi-zy4(t),它位于水稻第4染色体长臂上SSR标记RM5503与RM3276之间约318 kb区间内。【结论】Pi-zy4(t)为新的抗稻瘟病基因。研究结果为子预44的育种利用和Pi-zy4(t)基因的克隆提供了重要信息。

关键词: 水稻, 稻瘟病, 抗稻瘟病基因, 基因定位

CLC Number:

S435.111.4⁺1

Xiaoxuan ZHUO, Linlin FAN, Xingyu AN, Jingwei GUO, Rui YANG, Qianchun ZENG, Qiong LUO. Mapping of a New Rice Blast Resistance Gene in Ziyu 44, a Rice Landrace from Yunnan Province, China[J]. Chinese Journal OF Rice Science, 2019, 33(1): 12-19.

卓晓轩, 樊琳琳, 安星宇, 郭敬玮, 杨睿, 曾千春, 罗琼. 云南地方品种子预44中一个新的抗稻瘟病基因的定位[J]. 中国水稻科学, 2019, 33(1): 12-19.

Figures/Tables 6

References 47

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亲本或世代 Parent or progeny	总株数 Number of total plants	抗病单株 Resistant plants	感病单株 Susceptible plants	期望比 Expectation ratio	χ_c²
子预44 Ziyu 44	20	20	0
江南香糯 Jiangnanxiangnuo	20	0	20
F₂	149	116	33	3:1	0.55

亲本或世代 Parent or progeny	总株数 Number of total plants	抗病单株 Resistant plants	感病单株 Susceptible plants	期望比 Expectation ratio	χ_c²
子预44 Ziyu 44	20	20	0
江南香糯 Jiangnanxiangnuo	20	0	20
F₂	149	116	33	3:1	0.55

登录号	位置	蛋白
Accession	Location/bp	Protein
LOC_Os04g51880	30 770 723-30 764 486	GHMP激酶ATP结合蛋白GHMP kinases ATP-binding protein
LOC_Os04g51890	30 783 636-30 784 530	OsSAUR20-生长素应答SAUR基因家族成员OsSAUR20-Auxin-responsive SAUR gene family member
LOC_Os04g51900	30 791 816-30 797 525	表达蛋白Expressed protein
LOC_Os04g51910	30 803 061-30 798 724	蛋白酶体/环状体重复包含蛋白Proteasome/cyclosome repeat containing protein
LOC_Os04g51920	30 808 467-30 805 662	蛋白质二硫键异构酶Protein disulfide isomerase
LOC_Os04g51930	30 811 261-30 816 943	反转录转座子蛋白,LINE亚类Retrotransposon protein, putative, LINE subclass
LOC_Os04g51940	30 821 130-30 825 520	RNA结合蛋白YT521-B RNA binding protein YT521-B
LOC_Os04g51950	30 829 581-30 825 556	丝氨酸/苏氨酸蛋白激酶HT1 Serine/threonine-protein kinase HT1
LOC_Os04g51960	30 843 035-30 845 776	表达蛋白Expressed protein
LOC_Os04g51970	30 856 049-30 852 287	突触小泡2相关蛋白Synaptic vesicle 2-related protein
LOC_Os04g51980	30 860 607-30 863 364	转移酶家族结构域包含蛋白Transferase family domain containing protein
LOC_Os04g51990	30 869 666-30 871 743	转移酶家族结构域包含蛋白Transferase family domain containing protein
LOC_Os04g52000	30 879 563-30 875 218	蛋白磷酸酶2C Protein phosphatase 2C
LOC_Os04g52020	30 903 920-30 888 156	PHD-指结构域包含蛋白PHD-finger domain containing protein
LOC_Os04g52030	30 907 663-30 904 539	锌指结构域,LSD1亚类家族蛋白Zinc finger domain, LSD1 subclass family protein
LOC_Os04g52040	30 913 702-30 908 422	转换蛋白1 Transition protein 1(TNP1)
LOC_Os04g52050	30 9193 18-30 914 219	转换蛋白1 Transition protein 1(TNP1)
LOC_Os04g52060	30 925 574-30 920 554	转座因子蛋白Transposable element protein, putative, containing Pfam profile: PF03004, Transposase_24
LOC_Os04g52074	30 927 631-30 934 100	表达蛋白Expressed protein
LOC_Os04g52090	30 939 177-30 940 275	AP2结构域包含蛋白AP2 domain containing protein
LOC_Os04g52100	30 948 691-30 945 118	肽酶,M24家族蛋白Peptidase, M24 family protein
LOC_Os04g52110	30 950 939-30 952 114	晚期胚胎发育丰富的蛋白,第3组Late embryogenesis abundant protein, group 3
LOC_Os04g52120	30 957 619-30 952 498	钾转运蛋白Potassium transporter
LOC_Os04g52130	30 961 604-30 965 503	粪卟啉原Ⅲ氧化酶,叶绿体前体Coproporphyrinogen Ⅲ oxidase, chloroplast precursor
LOC_Os04g52140	30 974 112-30 979 849	蛋白激酶结构域包含蛋白Protein kinase domain containing protein
LOC_Os04g52164	30 995 338-30 999 600	转移酶家族蛋白Transferase family protein
LOC_Os04g52180	31 000 118-31 001 986	表达蛋白Expressed protein
LOC_Os04g52190	31 003 843-31 008 092	液泡分选受体前体Vacuolar-sorting receptor precursor
LOC_Os04g52200	31 013 915-31 007 297	RNA识别基序包含蛋白RNA recognition motif containing protein
LOC_Os04g52210	31 019 367-31 024 260	萜烯合成酶Terpene synthase
LOC_Os04g52220	31 024 637-31 025 064	表达蛋白Expressed protein
LOC_Os04g52230	31 026 617-31 031 248	ent-贝壳杉烯合酶,叶绿体前体ent-kaurene synthase, chloroplast precursor
LOC_Os04g52240	31 035 046-31 043 425	萜烯合成酶Terpene synthase
LOC_Os04g52250	31 051 171-31 052 039	LTPL123-蛋白酶抑制子/种子储存/ LTP家族蛋白前体 LTPL123-protease inhibitor/seed storage/LTP family protein precursor
LOC_Os04g52260	31 061 682-31 062 703	LTPL124-蛋白酶抑制剂/种子储存/ LTP家族蛋白前体 LTPL124-protease inhibitor/seed storage/LTP family protein precursor
LOC_Os04g52270	31 068 874-31 063 845	衣被蛋白ε亚基 ε coatomer subunit epsilon
LOC_Os04g52280	31 069 545-31 075 601	脱氢酶Dehydrogenase
LOC_Os04g52290	31 076 344-31 073 380	PPR重复结构域包含蛋白PPR repeat domain containing protein
LOC_Os04g52300	31 076 461-31 077 928	表达蛋白Expressed protein
LOC_Os04g52310	31 080 558-31 078200	金属阳离子转运蛋白Metal cation transporter

登录号	位置	蛋白
Accession	Location/bp	Protein
LOC_Os04g51880	30 770 723-30 764 486	GHMP激酶ATP结合蛋白GHMP kinases ATP-binding protein
LOC_Os04g51890	30 783 636-30 784 530	OsSAUR20-生长素应答SAUR基因家族成员OsSAUR20-Auxin-responsive SAUR gene family member
LOC_Os04g51900	30 791 816-30 797 525	表达蛋白Expressed protein
LOC_Os04g51910	30 803 061-30 798 724	蛋白酶体/环状体重复包含蛋白Proteasome/cyclosome repeat containing protein
LOC_Os04g51920	30 808 467-30 805 662	蛋白质二硫键异构酶Protein disulfide isomerase
LOC_Os04g51930	30 811 261-30 816 943	反转录转座子蛋白,LINE亚类Retrotransposon protein, putative, LINE subclass
LOC_Os04g51940	30 821 130-30 825 520	RNA结合蛋白YT521-B RNA binding protein YT521-B
LOC_Os04g51950	30 829 581-30 825 556	丝氨酸/苏氨酸蛋白激酶HT1 Serine/threonine-protein kinase HT1
LOC_Os04g51960	30 843 035-30 845 776	表达蛋白Expressed protein
LOC_Os04g51970	30 856 049-30 852 287	突触小泡2相关蛋白Synaptic vesicle 2-related protein
LOC_Os04g51980	30 860 607-30 863 364	转移酶家族结构域包含蛋白Transferase family domain containing protein
LOC_Os04g51990	30 869 666-30 871 743	转移酶家族结构域包含蛋白Transferase family domain containing protein
LOC_Os04g52000	30 879 563-30 875 218	蛋白磷酸酶2C Protein phosphatase 2C
LOC_Os04g52020	30 903 920-30 888 156	PHD-指结构域包含蛋白PHD-finger domain containing protein
LOC_Os04g52030	30 907 663-30 904 539	锌指结构域,LSD1亚类家族蛋白Zinc finger domain, LSD1 subclass family protein
LOC_Os04g52040	30 913 702-30 908 422	转换蛋白1 Transition protein 1(TNP1)
LOC_Os04g52050	30 9193 18-30 914 219	转换蛋白1 Transition protein 1(TNP1)
LOC_Os04g52060	30 925 574-30 920 554	转座因子蛋白Transposable element protein, putative, containing Pfam profile: PF03004, Transposase_24
LOC_Os04g52074	30 927 631-30 934 100	表达蛋白Expressed protein
LOC_Os04g52090	30 939 177-30 940 275	AP2结构域包含蛋白AP2 domain containing protein
LOC_Os04g52100	30 948 691-30 945 118	肽酶,M24家族蛋白Peptidase, M24 family protein
LOC_Os04g52110	30 950 939-30 952 114	晚期胚胎发育丰富的蛋白,第3组Late embryogenesis abundant protein, group 3
LOC_Os04g52120	30 957 619-30 952 498	钾转运蛋白Potassium transporter
LOC_Os04g52130	30 961 604-30 965 503	粪卟啉原Ⅲ氧化酶,叶绿体前体Coproporphyrinogen Ⅲ oxidase, chloroplast precursor
LOC_Os04g52140	30 974 112-30 979 849	蛋白激酶结构域包含蛋白Protein kinase domain containing protein
LOC_Os04g52164	30 995 338-30 999 600	转移酶家族蛋白Transferase family protein
LOC_Os04g52180	31 000 118-31 001 986	表达蛋白Expressed protein
LOC_Os04g52190	31 003 843-31 008 092	液泡分选受体前体Vacuolar-sorting receptor precursor
LOC_Os04g52200	31 013 915-31 007 297	RNA识别基序包含蛋白RNA recognition motif containing protein
LOC_Os04g52210	31 019 367-31 024 260	萜烯合成酶Terpene synthase
LOC_Os04g52220	31 024 637-31 025 064	表达蛋白Expressed protein
LOC_Os04g52230	31 026 617-31 031 248	ent-贝壳杉烯合酶,叶绿体前体ent-kaurene synthase, chloroplast precursor
LOC_Os04g52240	31 035 046-31 043 425	萜烯合成酶Terpene synthase
LOC_Os04g52250	31 051 171-31 052 039	LTPL123-蛋白酶抑制子/种子储存/ LTP家族蛋白前体 LTPL123-protease inhibitor/seed storage/LTP family protein precursor
LOC_Os04g52260	31 061 682-31 062 703	LTPL124-蛋白酶抑制剂/种子储存/ LTP家族蛋白前体 LTPL124-protease inhibitor/seed storage/LTP family protein precursor
LOC_Os04g52270	31 068 874-31 063 845	衣被蛋白ε亚基 ε coatomer subunit epsilon
LOC_Os04g52280	31 069 545-31 075 601	脱氢酶Dehydrogenase
LOC_Os04g52290	31 076 344-31 073 380	PPR重复结构域包含蛋白PPR repeat domain containing protein
LOC_Os04g52300	31 076 461-31 077 928	表达蛋白Expressed protein
LOC_Os04g52310	31 080 558-31 078200	金属阳离子转运蛋白Metal cation transporter