Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (1): 73-81.DOI: 10.3969/j.issn.1001-7216.2015.01.009
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Hua ZHAO1,3, Jun-min WANG2, Qi-fang ZHANG3, Qian ZHAO3, Shu-fang MEI1, Xiang-lei LIU1, Fang-min CHENG3,*()
Received:
2014-08-27
Revised:
2014-11-03
Online:
2015-01-10
Published:
2015-01-10
Contact:
Fang-min CHENG
赵华1,3, 王俊敏2, 张其芳3, 赵倩3, 梅淑芳1, 刘向蕾1, 程方民3,*()
通讯作者:
程方民
基金资助:
CLC Number:
Hua ZHAO, Jun-min WANG, Qi-fang ZHANG, Qian ZHAO, Shu-fang MEI, Xiang-lei LIU, Fang-min CHENG. Activities of Several Starch Synthesis Enzymes in Filling Grains for Rice Sugary Endosperm Mutant (Sug-11)and Its Relation to Starch Quality[J]. Chinese Journal OF Rice Science, 2015, 29(1): 73-81.
赵华, 王俊敏, 张其芳, 赵倩, 梅淑芳, 刘向蕾, 程方民. 水稻糖质胚乳突变体Sug-11籽粒灌浆过程的淀粉合成关键酶活性及其与淀粉理化特性关系[J]. 中国水稻科学, 2015, 29(1): 73-81.
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URL: http://www.ricesci.cn/EN/10.3969/j.issn.1001-7216.2015.01.009
基因名 Gene name | 登录号 Accession No. | 引物 Primer pairs | 产物大小 Product size/ bp |
---|---|---|---|
Actin | XM_469569 | F:5’-CAGCACATTCCAGCAGATGT | 198 |
R:5’-TAGGCCGGTTGAAAACTTTG | |||
ISA1 | AB093426 | F:5’-GTTGGAACCGTGTGGTGGAT | 112 |
R:5’-AGCAGAATGGAAGAGTATGGAGC | |||
ISA2 | AC132483 | F:5’-CAGTGAGTGCTGCCTTGC | 106 |
R:5’-TTGGGATTAGATTCGGTTG | |||
ISA3 | AP005574 | F:5’-CGCAGACTTCCCAACAGC | 144 |
R:5’-CCTTTCGTTTCATCTTTCGTG | |||
PUL | AB012915 | F:5’-ACCTTTCTTCCATGCTGG | 202 |
R:5’-CAAAGGTCTGAAAGATGGG |
Table 1 Primer sequences of four debranching enzyme(DBE) isoforms involving in starch biosynthesis pathway in rice grains.
基因名 Gene name | 登录号 Accession No. | 引物 Primer pairs | 产物大小 Product size/ bp |
---|---|---|---|
Actin | XM_469569 | F:5’-CAGCACATTCCAGCAGATGT | 198 |
R:5’-TAGGCCGGTTGAAAACTTTG | |||
ISA1 | AB093426 | F:5’-GTTGGAACCGTGTGGTGGAT | 112 |
R:5’-AGCAGAATGGAAGAGTATGGAGC | |||
ISA2 | AC132483 | F:5’-CAGTGAGTGCTGCCTTGC | 106 |
R:5’-TTGGGATTAGATTCGGTTG | |||
ISA3 | AP005574 | F:5’-CGCAGACTTCCCAACAGC | 144 |
R:5’-CCTTTCGTTTCATCTTTCGTG | |||
PUL | AB012915 | F:5’-ACCTTTCTTCCATGCTGG | 202 |
R:5’-CAAAGGTCTGAAAGATGGG |
年份与基因型 Year and genotype | 株高 Plant height/cm | 每穗实粒数 Number of filled grains per panicle | 千粒重 Grain weight/g | 结实率 Setting-rate /% | 蔗糖含量 Sucrose content/% | 可溶性总糖含量 Total soluble sugar contents/% | 淀粉含量 Starch content/% |
---|---|---|---|---|---|---|---|
2011 | |||||||
中花11 Zhonghua 11 | 90.6±4.6 | 132.6±8.2 | 24.4±0.5 | 83.1±3.9 | 1.73±0.15 | 1.99±0.13 | 76.83±1.87 |
Sug-11 | 86.9±3.9 | 107.3±6.6 | 20.6±0.3 | 75.9±6.7 | 5.40±0.49 | 5.87±0.22 | 71.35±0.89 |
差值Difference | 3.7 | 25.3** | 3.8** | 7.2* | -3.67** | -3.88** | 5.48** |
2012 | |||||||
中花11 Zhonghua 11 | 97.6±4.9 | 127.2±7.7 | 25.5±0.3 | 88.7±4.6 | 1.63±0.41 | 1.82±0.54 | 75.16±0.91 |
Sug-11 | 95.5±4.0 | 119.3±10.2 | 19.6±0.6 | 83.3±5.5 | 5.76±0.53 | 5.91±0.61 | 72.51±1.02 |
差值 Difference | 2.1 | 8.8 | 5.9** | 5.4 | -4.13** | 4.09** | 2.65* |
Table 2 Comparison of sucrose content, total soluble sugar contents, plant height and other agronomic traits between Sug-11 mutant and its wild type.
年份与基因型 Year and genotype | 株高 Plant height/cm | 每穗实粒数 Number of filled grains per panicle | 千粒重 Grain weight/g | 结实率 Setting-rate /% | 蔗糖含量 Sucrose content/% | 可溶性总糖含量 Total soluble sugar contents/% | 淀粉含量 Starch content/% |
---|---|---|---|---|---|---|---|
2011 | |||||||
中花11 Zhonghua 11 | 90.6±4.6 | 132.6±8.2 | 24.4±0.5 | 83.1±3.9 | 1.73±0.15 | 1.99±0.13 | 76.83±1.87 |
Sug-11 | 86.9±3.9 | 107.3±6.6 | 20.6±0.3 | 75.9±6.7 | 5.40±0.49 | 5.87±0.22 | 71.35±0.89 |
差值Difference | 3.7 | 25.3** | 3.8** | 7.2* | -3.67** | -3.88** | 5.48** |
2012 | |||||||
中花11 Zhonghua 11 | 97.6±4.9 | 127.2±7.7 | 25.5±0.3 | 88.7±4.6 | 1.63±0.41 | 1.82±0.54 | 75.16±0.91 |
Sug-11 | 95.5±4.0 | 119.3±10.2 | 19.6±0.6 | 83.3±5.5 | 5.76±0.53 | 5.91±0.61 | 72.51±1.02 |
差值 Difference | 2.1 | 8.8 | 5.9** | 5.4 | -4.13** | 4.09** | 2.65* |
淀粉指标 Starch parameters | 中花11 Zhonghua 11 | Sug-11 | 差值 Difference | ||||
---|---|---|---|---|---|---|---|
直链淀粉含量 Amylose content/% | 17.74 | ± | 0.68 | 10.46 | ± | 0.61 | 7.28** |
淀粉膨胀势 Swelling power/(g·g-1) | 13.68 | ± | 0.31 | 9.26 | ± | 0.57 | 4.45** |
淀粉溶解度 Starch solubility/% | 4.06 | ± | 0.51 | 24.95 | ± | 0.58 | -20.89** |
直链淀粉最大吸收波长 λmax.Am | 620.2 | ± | 2.0 | 608.0 | ± | 1.3 | 12.0** |
直链淀粉碘蓝值 BVAm | 0.39 | ± | 0.01 | 0.35 | ± | 0.03 | 0.04* |
支链淀粉最大吸收波长λmax.Ap | 589.3 | ± | 2.1 | 600.4 | ± | 2.2 | -11.1** |
支链淀粉碘蓝值 BVAp | 0.09 | ± | 0.01 | 0.12 | ± | 0.04 | -0.03 |
Table 3 Difference in starch physico-chemical parameters between Sug-11 mutant and its wild type(2012).
淀粉指标 Starch parameters | 中花11 Zhonghua 11 | Sug-11 | 差值 Difference | ||||
---|---|---|---|---|---|---|---|
直链淀粉含量 Amylose content/% | 17.74 | ± | 0.68 | 10.46 | ± | 0.61 | 7.28** |
淀粉膨胀势 Swelling power/(g·g-1) | 13.68 | ± | 0.31 | 9.26 | ± | 0.57 | 4.45** |
淀粉溶解度 Starch solubility/% | 4.06 | ± | 0.51 | 24.95 | ± | 0.58 | -20.89** |
直链淀粉最大吸收波长 λmax.Am | 620.2 | ± | 2.0 | 608.0 | ± | 1.3 | 12.0** |
直链淀粉碘蓝值 BVAm | 0.39 | ± | 0.01 | 0.35 | ± | 0.03 | 0.04* |
支链淀粉最大吸收波长λmax.Ap | 589.3 | ± | 2.1 | 600.4 | ± | 2.2 | -11.1** |
支链淀粉碘蓝值 BVAp | 0.09 | ± | 0.01 | 0.12 | ± | 0.04 | -0.03 |
Fig. 2. Difference in the temporal pattern of total soluble sugar, sucrose contents and starch accumulation between Sug-11 mutant and its wild type Zhonghua 11 in grains during grain filling.
Fig. 3. Difference in the temporal patterns of some key enzymes activities related to starch synthesis pathway in filling grains between Sug-11 mutant and its wild type Zhonghua 11. ADPGase, Adenosine diphosphoglucose pyrophosphprylase; SSS, Soluble starch synthase; SBE, Starch branching enzyme; DBE, Debranching enzyme.
Fig. 4. Transcriptional expressions and temporal pattern of 4 isoform genes encoding debranching enzyme(DBE) in grains during filling for Sug-11 mutant and its wild type Zhonghua 11.
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