Chinese Journal OF Rice Science ›› 2024, Vol. 38 ›› Issue (1): 106-110.DOI: 10.16819/j.1001-7216.2024.221203
• Experimental Technique • Previous Articles
WANG Jun1,3, ZHOU Jing2, TAO Yajun1,3, LI Wenqi1,3, ZHU Jianping1,3, FAN Fangjun1,3, WANG Fangquan1,3, XU Yang1,3, CHEN Zhihui1,3, JIANG Yanjie1,3, LI Xia1,3, YANG Jie1,3,*()
Received:
2022-12-09
Revised:
2023-02-16
Online:
2024-01-10
Published:
2024-01-16
Contact:
* email: 王军1,3, 周晶2, 陶亚军1,3, 李文奇1,3, 朱建平1,3, 范方军1,3, 王芳权1,3, 许扬1,3, 陈智慧1,3, 蒋彦婕1,3, 李霞1,3, 杨杰1,3,*()
通讯作者:
* email: 基金资助:
WANG Jun, ZHOU Jing, TAO Yajun, LI Wenqi, ZHU Jianping, FAN Fangjun, WANG Fangquan, XU Yang, CHEN Zhihui, JIANG Yanjie, LI Xia, YANG Jie. Development of HRM-based Functional Marker for Gelatinization Temperature Gene ALK in Rice[J]. Chinese Journal OF Rice Science, 2024, 38(1): 106-110.
王军, 周晶, 陶亚军, 李文奇, 朱建平, 范方军, 王芳权, 许扬, 陈智慧, 蒋彦婕, 李霞, 杨杰. 基于HRM技术开发水稻糊化温度基因ALK功能标记[J]. 中国水稻科学, 2024, 38(1): 106-110.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2024.221203
引物名称 Primer name | 引物序列 Primer sequence(5′-3′) | 片段大小 Expected size /bp | 扩增位点 Amplification site |
---|---|---|---|
ALKH-1F | TGGCGTACGGCACCGTCCC | 104 | A/G |
ALKH-1R | GTCGAACGTCCACCCGAGG | ||
ALKH-2F | GCCGCACAAGCTCATCGAG | 104 | TT/GC |
ALKH-2R | TGAGGTCCTGCGACATGCC | ||
ALKH-3F | TCGGCGGGCTGAGGGA | 69 | A/G |
ALKH-3R | CGAACGTCCACCCGAGGC | ||
ALKH-4F | CACCGTCCCCGTCGTG | 73 | A/G |
ALKH-4R | GGTGTCCTCGAACGGGTC | ||
ALKH-5F | CGTACCGCAAGTACAAGGAG | 66 | TT/GC |
ALKH-5R | AGCTGAGGTCCTGCGACA | ||
ALKH-6F | TCGAGACGTACCGCAAGTACAA | 82 | TT/GC |
ALKH-6R | GCGTGGTCCCAGCTGAGG | ||
ALKC-1F | GGTGGGGTTCTCGGTGAAG | 687 | 测序 Sequencing |
ALKC-1R | GCATCAATGGACATAACAAACAC |
Table 1. Functional markers and sequencing primers designed based on nucleotide polymorphism.
引物名称 Primer name | 引物序列 Primer sequence(5′-3′) | 片段大小 Expected size /bp | 扩增位点 Amplification site |
---|---|---|---|
ALKH-1F | TGGCGTACGGCACCGTCCC | 104 | A/G |
ALKH-1R | GTCGAACGTCCACCCGAGG | ||
ALKH-2F | GCCGCACAAGCTCATCGAG | 104 | TT/GC |
ALKH-2R | TGAGGTCCTGCGACATGCC | ||
ALKH-3F | TCGGCGGGCTGAGGGA | 69 | A/G |
ALKH-3R | CGAACGTCCACCCGAGGC | ||
ALKH-4F | CACCGTCCCCGTCGTG | 73 | A/G |
ALKH-4R | GGTGTCCTCGAACGGGTC | ||
ALKH-5F | CGTACCGCAAGTACAAGGAG | 66 | TT/GC |
ALKH-5R | AGCTGAGGTCCTGCGACA | ||
ALKH-6F | TCGAGACGTACCGCAAGTACAA | 82 | TT/GC |
ALKH-6R | GCGTGGTCCCAGCTGAGG | ||
ALKC-1F | GGTGGGGTTCTCGGTGAAG | 687 | 测序 Sequencing |
ALKC-1R | GCATCAATGGACATAACAAACAC |
Fig. 3. Sequence of ALK gene functional domains for partial rice varieties. A~D, Lianjing 18201, Runyangdao 1892, Yangfujing 1 and F2 plant, respectively. Red box indicates 4198 bp, 4329 bp and 4330 bp of ALK gene.
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