Chinese Journal OF Rice Science ›› 2023, Vol. 37 ›› Issue (2): 142-152.DOI: 10.16819/j.1001-7216.2023.220603

• Research Papers • Previous Articles     Next Articles

Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice

WEI Qianqian1,2, XU Qingshan2, PAN Lin2, KONG Yali2, ZHU Lianfeng2, CAO Xiaochuang2, TIAN Wenhao2, LIU Jia3, JIN Qianyu2, XIANG Xingjia1, ZHANG Junhua2,*(), ZHU Chunquan2,*()   

  1. 1School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China
    2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
    3Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
  • Received:2022-06-08 Revised:2022-08-31 Online:2023-03-10 Published:2023-03-10
  • Contact: ZHANG Junhua, ZHU Chunquan

钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制

魏倩倩1,2, 徐青山2, 潘林2, 孔亚丽2, 朱练峰2, 曹小闯2, 田文昊2, 刘佳3, 金千瑜2, 项兴佳1, 张均华2,*(), 朱春权2,*()   

  1. 1安徽大学 资源与环境工程学院, 合肥 230601
    2中国水稻研究所 水稻生物学国家重点实验室, 杭州 310006
    3江西省农业科学院 土壤肥料与资源环境研究所, 南昌 330200
  • 通讯作者: 张均华,朱春权
  • 基金资助:
    国家自然科学基金青年项目(31901452);国家自然科学基金青年项目(42207415);国家自然科学基金青年项目(32201702);国家自然科学基金面上项目(31872857);国家自然科学基金面上项目(31771733);国家重点研发计划课题(2022YFD1500403);国家重点研发计划课题(2022YFD2301404-2);水稻生物学国家重点实验室开放课题资助项目(20190402)

Abstract:

【Objective】 The objective of this study is to elucidate the molecular and physiological mechanisms of the interaction between calcium ion (Ca2+) and hydrogen sulfide (H2S) alleviating aluminum (Al) toxicity in rice.【Method】 The rice cultivar kasalath was selected as test material, and treated with 0 μmol/L and 30 μmol/L AlCl3, 0.1 mmol/L and 0.5 mmol/L CaCl2, 0.2 μmol/L NaHS and 100 μmol/L H2S scavenger hypotaurine (HP). The seeds were incubated at 30℃ in the dark for 24 h, then the root elongation, total Al content, Al content in cell sap, Al content in apoplast, Al content in cell wall, pectin content, pectin methylesterase activity and the relative expression of OsSTAR2, OsNRAT1 and OsFRDL4 were measured to explore the molecular and physiological mechanism of the interaction between Ca2+ and H2S alleviating Al toxicity in rice. 【Result】 Under Al stress, compared with 0.1 mmol/L CaCl2 treatment, 0.5 mmol/L CaCl2 treatment significantly increased root elongation, H2S content, total calcium (Ca) content and cytoplasm Ca content, and significantly decreased total Al content in root, cell sap Al content, apoplastic Al content and cell wall Al content in rice. The pretreatment of NaHS significantly increased the elongation of rice roots at the two calcium concentrations, accompanied with the significant increase of root tip Al content, root total Al content, cell sap Al content, apoplastic Al content and cell wall Al content. The relative expression levels of OsSTAR2 and OsFRDL4 were significantly increased and the relative expression of OsNRAT1 was significantly decreased at the two Ca2+ concentrations after pretreated with NaHS. However, the application of HP produced an opposite result. 【Conclusion】 Under Al stress, Ca2+ reduced the accumulation of Al in rice root cell wall and the absorption of Al by rice roots through increasing the production of H2S in rice root, and finally alleviated the inhibition of Al on rice root elongation.

Key words: rice, aluminum stress, calcium ion, hydrogen sulfide

摘要:

【目的】阐明钙离子与硫化氢相互作用缓解水稻铝毒害的分子和生理机制。【方法】以Kasalath为试验材料,选取0 μmol/L和30 μmol/L AlCl3,0.1 mmol/L 和 0.5 mmol/L CaCl2,0.2 μmol/L NaHS和100 μmol/L硫化氢清除剂亚牛磺酸(HP)作为处理浓度,将种子置于30℃培养箱中黑暗培养24 h后取水稻根系,通过测定水稻根系伸长量、总铝含量、细胞汁液中铝含量、质外体中铝含量、细胞壁中铝含量、果胶含量、果胶甲酯酶活性以及OsSTAR2OsNRAT1OsFRDL4相对表达量,探究钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制。【结果】铝胁迫下,相较于0.1 mmol/L CaCl2处理,0.5 mmol/L CaCl2处理显著提高了水稻根系伸长量、硫化氢含量、总钙含量和细胞质中钙含量,显著降低了水稻根系的总铝含量,细胞液、质外体和细胞壁中的铝含量。铝胁迫下,硫氢化钠预处理后,水稻根系的伸长量在两种钙浓度下均显著增加,水稻的根尖铝含量、根系总铝含量、细胞液中铝含量、质外体中铝含量和细胞壁中铝含量在两种钙浓度下均显著降低,OsSTAR2OsFRDL4相对表达量在两种钙浓度下均显著提高,OsNRAT1相对表达量在两种钙浓度下均显著降低。铝胁迫下,添加HP则呈现相反的结果。【结论】铝胁迫下,钙离子通过增加水稻根系硫化氢的生成,降低水稻根系对铝的吸收和积累,最终缓解铝对水稻根系伸长的抑制作用。

关键词: 水稻, 铝胁迫, 钙离子, 硫化氢