Chinese Journal OF Rice Science ›› 2023, Vol. 37 ›› Issue (2): 142-152.DOI: 10.16819/j.1001-7216.2023.220603
• Research Papers • Previous Articles Next Articles
WEI Qianqian1,2, XU Qingshan2, PAN Lin2, KONG Yali2, ZHU Lianfeng2, CAO Xiaochuang2, TIAN Wenhao2, LIU Jia3, JIN Qianyu2, XIANG Xingjia1, ZHANG Junhua2,*(), ZHU Chunquan2,*()
Received:
2022-06-08
Revised:
2022-08-31
Online:
2023-03-10
Published:
2023-03-10
Contact:
ZHANG Junhua, ZHU Chunquan
魏倩倩1,2, 徐青山2, 潘林2, 孔亚丽2, 朱练峰2, 曹小闯2, 田文昊2, 刘佳3, 金千瑜2, 项兴佳1, 张均华2,*(), 朱春权2,*()
通讯作者:
张均华,朱春权
基金资助:
WEI Qianqian, XU Qingshan, PAN Lin, KONG Yali, ZHU Lianfeng, CAO Xiaochuang, TIAN Wenhao, LIU Jia, JIN Qianyu, XIANG Xingjia, ZHANG Junhua, ZHU Chunquan. Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice[J]. Chinese Journal OF Rice Science, 2023, 37(2): 142-152.
魏倩倩, 徐青山, 潘林, 孔亚丽, 朱练峰, 曹小闯, 田文昊, 刘佳, 金千瑜, 项兴佳, 张均华, 朱春权. 钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制[J]. 中国水稻科学, 2023, 37(2): 142-152.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2023.220603
引物 Primer | 序列Sequence (5′-3′) |
---|---|
OsSTAR2-R | CCTCAGCTTCTTCATCGTCACC |
OsSTAR2-F | ACCTCTTCATGGTCACCGTCG |
OsFRDL4-R | TCATTTGCGAAGAAACTTCCACG |
OsFRDL4-F | CGTCATCAGCACCATCCACAG |
OsNRAT1-F | GAGGCCGTCTGCAGGAGAGG |
OsNRAT1-R | GGAAGTATCTGCAAGCAGCTCTGATGC |
OsHistone-R | AACCGCAAAATCCAAAGAACG |
OsHistone-F | GGTCAACTTGTTGATTCCCCTCT |
Table 1. Primers selected for this study and the sequences.
引物 Primer | 序列Sequence (5′-3′) |
---|---|
OsSTAR2-R | CCTCAGCTTCTTCATCGTCACC |
OsSTAR2-F | ACCTCTTCATGGTCACCGTCG |
OsFRDL4-R | TCATTTGCGAAGAAACTTCCACG |
OsFRDL4-F | CGTCATCAGCACCATCCACAG |
OsNRAT1-F | GAGGCCGTCTGCAGGAGAGG |
OsNRAT1-R | GGAAGTATCTGCAAGCAGCTCTGATGC |
OsHistone-R | AACCGCAAAATCCAAAGAACG |
OsHistone-F | GGTCAACTTGTTGATTCCCCTCT |
Fig. 1. Effects of different Al3+ concentrations on root elongation (A) and Al content in root apices (B). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3).
Fig. 2. Effects of different Ca2+ concentrations on root elongation (A) and Al content in root apices (B). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). -Al, No Al3+ addition; +Al, 30μmol/L Al3+.
Fig. 3. Effects of different Ca2+ concentrations on total Al concentration (A), Al concentration in cell sap (B), apoplastic Al concentration (C), Al content in cell wall(D), pectin content (E), pectin methylesterase activity (F). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.5Ca, 0.5 mmol/L CaCl2; 0.1Ca+Al, 0.1 mmol/L CaCl2+30 μmol/L AlCl3; 0.5Ca+Al, 0.5 mmol/L CaCl2+30 μmol/L AlCl3.
Fig. 4. Effects of different Ca2+ concentrations on total Ca concentration (A), Ca concentration in cell sap (B and C). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca: 0.1 mmol/L CaCl2; 0.5Ca: 0.5 mmol/L CaCl2; 0.1Ca+Al: 0.1 mmol/L CaCl2+30 μmol/L AlCl3; 0.5Ca+Al: 0.5 mmol/L CaCl2+30 μmol/L AlCl3.
Fig. 5. Effects of different Ca2+ concentrations on the content of endogenous H2S. Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca: 0.1 mmol/L CaCl2; 0.5Ca: 0.5 mmol/L CaCl2; 0.1Ca+Al: 0.1 mmol/L CaCl2+30 μmol/L AlCl3; 0.5Ca+Al: 0.5 mmol/L CaCl2+30 μmol/L AlCl3.
Fig. 6. Effects of interaction between Ca2+ and H2S on root elongation (A), relative root elongation (B) and Al content in root apices (C). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.1Ca+NaHS, 0.1 mmol/L CaCl2+0.2 μmol/L NaHS; 0.1Ca+HP, 0.1 mmol/L CaCl2+100 μmol/L HP; 0.5Ca, 0.5 mmol/L CaCl2; 0.5Ca+NaHS, 0.5 mmol/L CaCl2+0.2 μmol/L NaHS; 0.5Ca+HP, 0.5 mmol/L CaCl2+100 μmol/L HP.
Fig. 7. Effects of interaction between Ca2+ and H2S on total Al concentration (A), Al concentration in cell sap (B), apoplastic Al concentration (C), Al content in cell wall(D), pectin content (E), pectin methylesterase activity (F). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.1Ca+NaHS, 0.1 mmol/L CaCl2+0.2 μmol/L NaHS; 0.1Ca+HP, 0.1 mmol/L CaCl2+100 μmol/L HP; 0.5Ca, 0.5 mmol/L CaCl2; 0.5Ca+NaHS, 0.5 mmol/L CaCl2+0.2 μmol/L NaHS; 0.5Ca+HP, 0.5 mmol/L CaCl2+100 μmol/L HP.
Fig. 8. Effects of interaction between Ca2+ and H2S on relative expression of OsSTAR2 (A), OsFRDL4 (B) and OsNRAT1 (C). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.1Ca+NaHS, 0.1 mmol/L CaCl2+0.2 μmol/L NaHS; 0.1Ca+HP, 0.1 mmol/L CaCl2+100 μmol/L HP; 0.5Ca, 0.5 mmol/L CaCl2; 0.5Ca+NaHS, 0.5 mmol/L CaCl2+0.2 μmol/L NaHS; 0.5Ca+HP, 0.5 mmol/L CaCl2+100 μmol/L HP.
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