Chinese Journal OF Rice Science ›› 2020, Vol. 34 ›› Issue (6): 512-519.DOI: 10.16819/j.1001-7216.2020.0406
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Yinhua MA1, Pingfang LI1, Wenjing DONG1, Songwang YI1, Fang YANG2, Bo DU2, Chenzhong JIN1,*()
Received:
2020-04-25
Revised:
2020-05-18
Online:
2020-11-10
Published:
2020-11-10
Contact:
Chenzhong JIN
马银花1, 李萍芳1, 董文静1, 易松望1, 杨芳2, 杜波2, 金晨钟1,*()
通讯作者:
金晨钟
基金资助:
CLC Number:
Yinhua MA, Pingfang LI, Wenjing DONG, Songwang YI, Fang YANG, Bo DU, Chenzhong JIN. Mechanism Analysis of Rice Resistance Protein OsRRK1 Againstthe Brown Planthopper[J]. Chinese Journal OF Rice Science, 2020, 34(6): 512-519.
马银花, 李萍芳, 董文静, 易松望, 杨芳, 杜波, 金晨钟. 水稻抗性蛋白OsRRK1抗褐飞虱机理分析[J]. 中国水稻科学, 2020, 34(6): 512-519.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2020.0406
引物 Primer | 引物序列(5'-3') Primer sequence(5'-3') | 限制性内切酶酶切位点 Restriction endonuclease site |
---|---|---|
AD-LecRKF | CGGAATTCATGGTTGCTCTGCTACTCTTT | EcoR I |
AD-LecRKR | CGGAATTCTTAATGAGAAAACAGAGGATTACATTCC | EcoR I |
AD-LecRK1F | TCCCCCGGGATGGTTGCTCTGCTACTCTTTCCC | Sma I |
AD-LecRK1R | TCCCCCGGGCTATGGAAGTGAGCTGATGAAGG | Sma I |
AD-LecRK2F | TCCCCCGGGTATGGCACCTATCCTGTTCTTGCCC | Sma I |
AD-LecRK2R | TCCCCCGGGTCATGCGAGTGAACTGATATAGG | Sma I |
AD-LecRK3F | TCCCCCGGGTATGGCTCATCTCCTGTTCTTGCCC | Sma I |
AD-LecRK3R | TCCCCCGGGTCATGCAAGTGAACTGATATAGG | SmaI |
BK-OsRRK1F | CGGAATTCATGAGGCCTCTGTACCTGCGCAGC | EcoR I |
BK-OsRRK1R | CGGAATTCCTAATTGCTCAAAGATGATGAGCA | EcoR I |
T7 | TAATACGACTCACTATAGGGCGA | |
3′AD | AGATGGTGCACGATGCACAG | |
3′BD | TTTTCGTTTTCCTAAGAGTC |
Table 1 Primers used in vectors constructed.
引物 Primer | 引物序列(5'-3') Primer sequence(5'-3') | 限制性内切酶酶切位点 Restriction endonuclease site |
---|---|---|
AD-LecRKF | CGGAATTCATGGTTGCTCTGCTACTCTTT | EcoR I |
AD-LecRKR | CGGAATTCTTAATGAGAAAACAGAGGATTACATTCC | EcoR I |
AD-LecRK1F | TCCCCCGGGATGGTTGCTCTGCTACTCTTTCCC | Sma I |
AD-LecRK1R | TCCCCCGGGCTATGGAAGTGAGCTGATGAAGG | Sma I |
AD-LecRK2F | TCCCCCGGGTATGGCACCTATCCTGTTCTTGCCC | Sma I |
AD-LecRK2R | TCCCCCGGGTCATGCGAGTGAACTGATATAGG | Sma I |
AD-LecRK3F | TCCCCCGGGTATGGCTCATCTCCTGTTCTTGCCC | Sma I |
AD-LecRK3R | TCCCCCGGGTCATGCAAGTGAACTGATATAGG | SmaI |
BK-OsRRK1F | CGGAATTCATGAGGCCTCTGTACCTGCGCAGC | EcoR I |
BK-OsRRK1R | CGGAATTCCTAATTGCTCAAAGATGATGAGCA | EcoR I |
T7 | TAATACGACTCACTATAGGGCGA | |
3′AD | AGATGGTGCACGATGCACAG | |
3′BD | TTTTCGTTTTCCTAAGAGTC |
Fig. 4. The interaction between OsRRK1 and OsLecRK protein was verified by yeast two-hybridization. pGBKT7-53/pGADT7-T, Positive control; pGBKT7-Lam/pGADT7-T, Negative control. DDO, SD/-Leu/-Trp; TDO, SD/-Leu/-Trp/-His.
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