Correlation Analysis and QTL Mapping for Starch RVA Profile Properties and Amylose and Protein Contents in Rice

doi:10.16819/j.1001-7216.2017.6046

Abstract

Abstract:

【Objective】The detection of new quantitative trait loci(QTLs)and the correlation analysis between different rice quality traits are used to dissect the genetic mechanism underlying rice quality and to breed high-quality rice varieties. 【Method】The backcross inbred lines(BILs) of Sasanishiki× Habataki planted in two environments were used to detect additive QTLs for amylose content, protein content and RVA profiles. 【Result】Phenotypic analysis showed the protein content of Habataki was higher than that of Sasanishiki while other rice quality parameters of Sasanishiki were generally higher than those of Habataki except the setback viscosity. A total of 42 additive QTLs were detected, and ten of them could be repeatedly detected in two environments, including qPC8, qAC4, qAC10, qPKV2, qPKV7, qHPV7, qCPV1, qBDV4, qBDV7 and qSBV7. Of them, qCPV1, qBDV4, qPKV7, qHPV7 and qAC10 had not been reported previously. Moreover, the environmental stability of 10 QTLs, was further confirmed across two different environments by chromosome segment substitution lines(CSSLs). 【Conclusion】Significant correlations were observed between RVA profiles and protein or amylose contents, and many QTLs affecting different quality traits were co-localized.

Key words: rice, grain quality, quantitative trait locus, backcross inbred lines, chromosome segment substitution lines

摘要：

目的检测到新的控制稻米品质性状相关的QTL并分析各性状间的相关性,为了解控制水稻品质的遗传机理和培育优质水稻品种奠定基础。方法利用Sasanishiki×Habataki回交重组自交系(backcross inbred lines, BILs)群体在两个环境下种植的结果,检测与稻米直链淀粉含量、蛋白质含量及RVA谱特征值相关的加性QTL。结果表型分析结果显示,Habataki的蛋白含量明显高于Sasanishiki;而除消减值以外其余的稻米品质性状指标,Sasanishiki均高于Habataki。利用BIL群体共检测到加性QTL 42个,其中10个QTL位点在2个环境中均能被检测到,即qPC8、qAC4、qAC10、qPKV2、qPKV7、qHPV7、qCPV1、qBDV4、qBDV7、qSBV7,且qCPV1、qBDV4、qPKV7、qHPV7和qAC10等5个QTL尚未见报道。同时,我们还利用Sasanishiki×Habataki染色体片断置换系(Chromosome segment substitution lines, CSSLs)验证了10个稳定表达的QTL位点。结论稻米RVA谱特征值与直链淀粉含量、蛋白质含量之间呈现一定相关性,且控制不同品质性状的QTL之间具有共定位现象。

关键词: 水稻, 稻米品质, 数量性状基因座, 回交重组自交系, 染色体片段置换系

Jie ZHANG, Leina ZHENG, Yue CAI, Xiaoman YOU, Fei KONG, Guoxiang WANG, Haigang YAN, Jie JIN, Liang WANG, Wenwei ZHANG, Ling JIANG. Correlation Analysis and QTL Mapping for Starch RVA Profile Properties and Amylose and Protein Contents in Rice[J]. Chinese Journal OF Rice Science, 2017, 31(1): 31-39.

张杰, 郑蕾娜, 蔡跃, 尤小满, 孔飞, 汪国湘, 燕海刚, 金洁, 王亮, 张文伟, 江玲. 稻米淀粉RVA谱特征值与直链淀粉、蛋白含量的相关性及QTL定位分析[J]. 中国水稻科学, 2017, 31(1): 31-39.

Figures/Tables 5

References 32

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性状 Trait	亲本(平均值±标准差) Parent(Mean±SD)		BIL群体 BIL population
	亲本(平均值±标准差) Parent(Mean±SD)		平均值±标准差 Mean±SD	变异范围 Range
	Sasanishiki		平均值±标准差 Mean±SD		Habataki
蛋白质含量 Protein content/%	7.15±0.01	8.09±0.02	7.96±0.78	6.52~9.94
	7.36±0.02	8.79±0.01	7.96±0.75	6.55~9.78
直链淀粉含量 Amylose content/%	14.18±0.11	12.89±0.22	13.09±2.14	9.58~24.87
	14.32±0.12	13.04±0.20	13.08±2.12	9.65~24.95
峰值黏度 Peak viscosity/BU	362.75±12.00	222.58±12.80	345.86±35.90	167.67~396.33
	301.83±4.00	279.25±3.00	299.32±21.89	244.50~349.25
热浆黏度 Hot paste viscosity/BU	229.75±8.00	102.42±6.90	206.62±25.30	134.75~267.08
	134.58±4.50	121.42±6.00	324.02±52.02	106.75~179.50
崩解值 Breakdown viscosity/BU	133.00±4.00	120.17±7.20	139.24±24.30	32.92~186.75
	167.25±0.50	157.83±3.00	212.26±120.06	114.50~186.58
冷胶黏度 Cool paste viscosity/BU	326.67±20.20	178.67±9.30	298.16±23.77	241.08~352.67
	235.75±1.50	225.33±1.00	416.30±53.47	202.42~314.67
消减值 Setback viscosity/BU	-36.08±8.10	-43.92±3.50	-47.70±35.24	-119.58~134.25
	-66.08±5.50	-53.92±2.00	-120.17±113.11	-108.00~29.50
回复值 Consistence viscosity/BU	96.92±12.20	76.25±2.40	91.54±14.86	61.66~167.17
	107.17±6.00	103.91±5.00	92.28±39.78	62.50~144.00
峰值时间 Peak time/min	6.27±0.03	5.73±0.08	6.21±0.18	5.73~6.53
	5.87±0.16	5.67±0.10	5.91±0.42	5.25~6.83

性状 Trait	亲本(平均值±标准差) Parent(Mean±SD)		BIL群体 BIL population
	亲本(平均值±标准差) Parent(Mean±SD)		平均值±标准差 Mean±SD	变异范围 Range
	Sasanishiki		平均值±标准差 Mean±SD		Habataki
蛋白质含量 Protein content/%	7.15±0.01	8.09±0.02	7.96±0.78	6.52~9.94
	7.36±0.02	8.79±0.01	7.96±0.75	6.55~9.78
直链淀粉含量 Amylose content/%	14.18±0.11	12.89±0.22	13.09±2.14	9.58~24.87
	14.32±0.12	13.04±0.20	13.08±2.12	9.65~24.95
峰值黏度 Peak viscosity/BU	362.75±12.00	222.58±12.80	345.86±35.90	167.67~396.33
	301.83±4.00	279.25±3.00	299.32±21.89	244.50~349.25
热浆黏度 Hot paste viscosity/BU	229.75±8.00	102.42±6.90	206.62±25.30	134.75~267.08
	134.58±4.50	121.42±6.00	324.02±52.02	106.75~179.50
崩解值 Breakdown viscosity/BU	133.00±4.00	120.17±7.20	139.24±24.30	32.92~186.75
	167.25±0.50	157.83±3.00	212.26±120.06	114.50~186.58
冷胶黏度 Cool paste viscosity/BU	326.67±20.20	178.67±9.30	298.16±23.77	241.08~352.67
	235.75±1.50	225.33±1.00	416.30±53.47	202.42~314.67
消减值 Setback viscosity/BU	-36.08±8.10	-43.92±3.50	-47.70±35.24	-119.58~134.25
	-66.08±5.50	-53.92±2.00	-120.17±113.11	-108.00~29.50
回复值 Consistence viscosity/BU	96.92±12.20	76.25±2.40	91.54±14.86	61.66~167.17
	107.17±6.00	103.91±5.00	92.28±39.78	62.50~144.00
峰值时间 Peak time/min	6.27±0.03	5.73±0.08	6.21±0.18	5.73~6.53
	5.87±0.16	5.67±0.10	5.91±0.42	5.25~6.83

指标 Index	蛋白质含量 PC	直链淀粉含量 AC	峰值黏度 PKV	热浆黏度 HPV	崩解值 BDV	冷胶黏度 CPV	消减值 SBV	回复值 CSV
直链淀粉含量 AC	-0.22
	-0.14
峰值黏度 PKV	-0.24	-0.60^**
	-0.17	-0.20
热浆黏度 HPV	-0.36^**	-0.23^*	0.74^**
	-0.39^**	-0.15	0.73^**
崩解值 BDV	-0.29^*	-0.64^**	0.71^**	0.05
	-0.26^*	-0.54^**	0.70^**	0.03
冷胶黏度 CPV	-0.38^**	0.26^*	0.36^**	0.82^**	-0.32^**
	-0.33^**	0.17	0.44^**	0.83^**	-0.21
消减值 SBV	0.19	0.78^**	-0.78^**	-0.20	-0.94^**	0.31^**
	0.12	0.86^**	-0.62^**	-0.10	-0.89^**	0.43^**
回复值 CSV	0.02	0.81^**	-0.68^**	-0.40^**	-0.60^**	0.21	0.83^**
	0.02	0.78^**	-0.27^*	-0.41^**	-0.41^**	0.58^**	0.78^**
峰值时间 PeT	0.07	0.46^**	-0.17	0.45^**	-0.72^**	0.69^**	0.64^**	0.34^**
	0.02	0.38^**	-0.27^*	0.41^**	-0.41^**	0.58^**	0.78^**	0.92^**

指标 Index	蛋白质含量 PC	直链淀粉含量 AC	峰值黏度 PKV	热浆黏度 HPV	崩解值 BDV	冷胶黏度 CPV	消减值 SBV	回复值 CSV
直链淀粉含量 AC	-0.22
	-0.14
峰值黏度 PKV	-0.24	-0.60^**
	-0.17	-0.20
热浆黏度 HPV	-0.36^**	-0.23^*	0.74^**
	-0.39^**	-0.15	0.73^**
崩解值 BDV	-0.29^*	-0.64^**	0.71^**	0.05
	-0.26^*	-0.54^**	0.70^**	0.03
冷胶黏度 CPV	-0.38^**	0.26^*	0.36^**	0.82^**	-0.32^**
	-0.33^**	0.17	0.44^**	0.83^**	-0.21
消减值 SBV	0.19	0.78^**	-0.78^**	-0.20	-0.94^**	0.31^**
	0.12	0.86^**	-0.62^**	-0.10	-0.89^**	0.43^**
回复值 CSV	0.02	0.81^**	-0.68^**	-0.40^**	-0.60^**	0.21	0.83^**
	0.02	0.78^**	-0.27^*	-0.41^**	-0.41^**	0.58^**	0.78^**
峰值时间 PeT	0.07	0.46^**	-0.17	0.45^**	-0.72^**	0.69^**	0.64^**	0.34^**
	0.02	0.38^**	-0.27^*	0.41^**	-0.41^**	0.58^**	0.78^**	0.92^**

位点 Loci	染色体 Chr.	标记区间 Marker interval			地点 Location	LOD值 LOD value	贡献率 PVE/%	加性效应 Effect	效应来源 Positive allele
qPC1	1	R1485	-	R886	E1	2.71	11.46	-0.47	Habataki
qPC8	8	R2382	-	S14074	E1	3.26	14.44	0.45	Sasanishiki
					E2	2.44	11.47	0.39	Sasanishiki
qPC9	9	R1751	-	G385	E1	2.25	10.23	-0.61	Habataki
qPC12.1	12	C1336	-	R367	E2	3.31	13.89	0.47	Sasanishiki
qPC12.2	12	S1436	-	R1709	E2	3.35	16.16	-0.44	Habataki
qAC4	4	G264	-	G177	E1	2.91	10.69	-1.16	Habataki
					E2	2.94	10.71	-1.15	Habataki
qAC5	5	R3166	-	R708	E1	2.01	7.70	1.24	Sasanishiki
qAC10	10	R2447	-	R3285	E1	3.82	16.38	-2.09	Habataki
					E2	4.00	16.97	-2.09	Habataki
qPKV2	2	R1843	-	S2068	E1	2.78	22.34	26.89	Sasanishiki
					E2	2.99	12.67	14.48	Sasanishiki
qPKV3	3	S1466	-	R19	E2	2.45	9.96	-11.87	Habataki
qPKV4	4	G271	-	C513	E2	2.20	10.13	10.51	Sasanishiki
qPKV7	7	R2829	-	R2401	E1	2.10	7.72	13.69	Sasanishiki
					E2	3.32	21.72	16.30	Sasanishiki
qPKV10	10	R2447	-	R3285	E1	2.35	8.74	16.30	Sasanishiki
qHPV2	2	C37	-	C1236	E2	3.35	15.32	11.53	Sasanishiki
qHPV7	7	R2829	-	R2401	E1	2.87	14.66	8.65	Sasanishiki
					E2	2.69	11.74	8.05	Sasanishiki
qCPV1	1	C470	-	R1944	E1	3.10	15.38	14.15	Sasanishiki
					E2	2.56	9.47	8.94	Sasanishiki
qCPV2	2	G1340	-	R1843	E1	2.39	11.31	13.39	Sasanishiki
qCPV8	8	R2382	-	S14074	E1	2.88	12.51	14.96	Sasanishiki
qCPV9	9	C711	-	G36	E2	2.40	15.68	-9.69	Habataki
qCPV10	10	R716	-	C16	E1	2.38	12.82	15.08	Sasanishiki
qBDV1	1	G393	-	C813	E1	2.51	8.86	-11.74	Habataki
qBDV2	2	R1843	-	S2068	E1	3.21	11.77	14.38	Sasanishiki
qBDV3	3	C1351	-	C1468	E2	2.14	7.87	-6.47	Habataki
qBDV4	4	G264	-	G177	E1	2.96	10.55	12.93	Sasanishiki
					E2	3.02	11.45	13.04	Sasanishiki
qBDV5	5	R3166	-	R708	E1	2.03	7.90	-11.59	Habataki
qBDV7	7	R2829	-	R2401	E1	2.98	17.66	8.97	Sasanishiki
					E2	3.24	19.36	9.43	Sasanishiki
qBDV8	8	R2382	-	S14074	E1	2.82	10.45	-11.47	Habataki
qBDV9	9	G36	-	R1146	E2	5.21	22.47	12.74	Sasanishiki
qSBV2	2	S2068	-	C499	E1	2.79	21.70	-26.49	Habataki
qSBV5	5	R708	-	R372	E2	2.29	9.39	11.84	Sasanishiki
qSBV7	7	R2829	-	R2401	E1	3.90	14.99	-19.42	Habataki
					E2	2.84	18.41	-13.72	Habataki
qSBV9	9	G36	-	R1146	E2	2.75	29.11	-18.62	Habataki
qSBV10	10	S2083	-	R2174	E1	2.80	10.50	-17.12	Habataki
qSBV12	12	C1336	-	R367	E2	2.73	11.73	-10.91	Habataki
qCSV1	1	R1613	-	S1778	E2	2.81	13.44	6.50	Sasanishiki
qCSV2	2	S2068	-	C499	E1	2.13	13.66	-11.18	Habataki
qCSV5	5	R708	-	R372	E1	2.80	10.61	8.30	Sasanishiki
qCSV7	7	R2829	-	R2401	E1	2.55	8.13	7.29	Sasanishiki
qCSV8	8	R1943	-	G278	E1	2.15	8.13	7.29	Sasanishiki
qPeT1.1	1	R1613	-	S1778	E2	2.81	13.44	6.50	Sasanishiki
qPeT1.2	1	G393	-	C813	E1	2.70	12.75	0.10	Sasanishiki
qPeT8	8	R2382	-	S14074	E1	3.49	20.47	0.13	Sasanishiki