中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

二化螟对杀虫单抗性现实遗传力与遗传方式

曹明章1, 2; 沈晋良1,*   

  1. 1南京农业大学 植物保护学院 农药系， 农业部病虫监测与治理重点开放实验室， 江苏 南京210095； 2江苏省农药研究所， 江苏 南京210036；*通讯联系人， E-mail: jlshen@public1.ptt.js.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-09-10 发布日期:2005-09-10

Realized Heritability and Inheritance Mode of Monosultap Resistance in Chilo suppressalis(Lepidoptera: Pyralidae)

CAO Ming-zhang , SHEN Jin-Liang   

  • Received:1900-01-01 Revised:1900-01-01 Online:2005-09-10 Published:2005-09-10

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被引次数

18

摘要： 以2001年田间采集的温州二化螟为起始种群，在室内饲养24代期间，用点滴法处理4龄幼虫进行杀虫单筛选19代，得到抗性品系WZR；与敏感基线相比， WZR品系对杀虫单抗性达774.5倍，比筛选前上升25.0倍；根据前期连续6代（F3~F8）筛选估计的抗性现实遗传力（h2，0.230）显著低于根据后期连续5代（F19~F23）筛选的h2（1205），而中间不连续筛选（F9~F18，10代中有4代未筛选）的h2最小（0.024）。用毒力回归线法对WZR品系进行抗性遗传分析表明，杀虫单抗性为不完全显性（正反交显性度分别为0.56和0.63）、常染色体遗传，抗性不属于单个主基因控制。

关键词: 二化螟, 抗性, 现实遗传力, 遗传, 杀虫单

Abstract: A colony of the striped stem borer,Chilo suppressalis,collected from Wenzhou,Zhejiang Province,was further selected in laboratory with monosultap using topical application method.After selection with monosultap for 19 generations in 24 generations of laboratory culture,resistance to monosultap in the selected strain(WZ-R) increased by 25.0-fold,and 774.5-fold compared with that of the pre-selected and the susceptible baseline of HLJ strain,respectively.The realized heritability(h2) estimated from early selection stage(0.230 for F3-F8,consecutive selection for 6 generations) was significantly smaller than that from later stage(1.205 for F19-F23,consecutive selection for 5 generations),while the h2 estimated from middle inconsecutive selection stage(F9-F18,during which there were a total of 4 generations without selection) was the smallest(0.024).Inheritance of monosultap resistance in WZ-R strain,studied by log dose-probit(LD-p) analysis for progenies of reciprocal crosses,two backcrosses and a F1 self-cross indicated that resistance to monosultap was inherited autosomaly in an incompletely dominant manner,with a degree of dominance for reciprocal cross F1s being 0.56 and 0.63,respectively,and monosultap resistance in WZ-R appeared to be controlled by two or more genes.

Key words: Chilo suppressalis, resistance, realized heritability, inheritance