中国水稻科学 ›› 2024, Vol. 38 ›› Issue (1): 25-32.DOI: 10.16819/j.1001-7216.2024.221206

• 研究报告 • 上一篇    下一篇

红米水稻抗氧化活性成分的遗传效应与环境互作

程祖锌1,2, 肖长春3, 张玉婷1,2, 黄昕颖1,2, 史夏蕾1,2, 凌波1,2, 王泓超1,2, 陈小玲1,2, 林荔辉1,2,*()   

  1. 1福建农林大学 农学院/福建省特种作物育种与利用工程技术研究中心, 福州 350002
    2福建农林大学 农学院/作物遗传育种与综合利用教育部重点实验室, 福州 350002
    3福建省(山区)作物遗传改良与创新利用重点实验室, 福建 三明 365500
  • 收稿日期:2022-12-14 修回日期:2023-05-30 出版日期:2024-01-10 发布日期:2024-01-16
  • 通讯作者: * email: lihui9027@163.com
  • 基金资助:
    福建省高校产学合作项目(2022N5011);福建省(山区)作物遗传改良与创新利用重点实验室开放课题资助项目(2022SKF02);福建省科技计划引导性项目(2022N0005);福建农林大学2019年度乡村振兴服务团队资助项目(11899170122)

Genetic Effects of Antioxidant Components in Red Rice and Its Interactions with Environment

CHENG Zuxin1,2, XIAO Changchun3, ZHANG Yuting1,2, HUANG Xinying1,2, SHI Xialei1,2, LING Bo1,2, WANG Hongchao1,2, CHEN Xiaoling1,2, LIN Lihui1,2,*()   

  1. 1Fujian Engineering Technology Research Center of Breeding and Utilization for Special Crops/College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    2Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops/College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    3Fujian Key Laboratory of Crop Genetic Improvement and Innovative Utilization for Mountain Area, Sanming 365500, China
  • Received:2022-12-14 Revised:2023-05-30 Online:2024-01-10 Published:2024-01-16
  • Contact: * email: lihui9027@163.com

摘要:

【目的】剖析红米水稻总酚、总黄酮、原花青素和花色苷的遗传效应,为高营养价值红米杂交稻的培育提供参考。【方法】以8个红米恢复系为父本,6个不育系为母本,配制48个杂交组合为遗传材料,利用加性-显性遗传模型(AD模型)及统计方法,分析在不同环境下红米水稻抗氧化活性成分的遗传效应、杂种优势、遗传相关及其与环境互作。【结果】花色苷、原花青素、总酚和总黄酮主要受遗传主效应控制,其中原花青素、总酚和总黄酮总狭义遗传率高,以基因加性效应为主,低代选择有效;花色苷以显性效应为主,适宜中、高世代选择。这4种抗氧化活性成分的表型、遗传、加性、显性、加性×环境互作和显性×环境互作均呈正向相关,相关系数大;原花青素的基因型×环境互作效应小,稳定性好,以此为选择指标可有效提高其他3种成分,减轻工作量,提高育种效率。高温有利红米水稻杂种后代抗氧化活性成分的积累,提高群体杂种优势。此外,提高不育系抗氧化活性成分含量,有利于培育高抗氧化活性成分含量的杂交后代;恢复系18Rr174、18Rr175可有效提高后代总酚、总黄酮、原花青素含量,且受环境影响小。【结论】采用植物数量性状的加性-显性遗传模型,可有效预测亲本抗氧化活性成分的遗传效应,18Rr174、18Rr175在高抗氧化活性成分红米杂交稻的选育上具有较高应用价值。

关键词: 红米水稻, 抗氧化成分, 遗传效应, 基因型×环境互作效应

Abstract:

【Objective】 This study aims to dissect the genetic effects of total phenols, total flavonoids, procyanidins, and anthocyanins in red rice, to offer insights for cultivating high-nutrition red hybrid rice. 【Methods】 Forty-eight hybrid combinations were generated using eight red rice restorer lines as male parents and six sterile lines as female parents. Employing the additive-dominance genetic model (AD model) and statistical methods, the study analyzed the genetic effects, heterosis, genetic correlation of antioxidant components in red rice across diverse environmental conditions, and their interactions with the environment. 【Results】 Genetic main effects primarily control anthocyanins, procyanidins, total phenols, and total flavonoids. Procyanidins, total phenols, and total flavonoids exhibit high narrow-sense heritability, mainly governed by gene additive effects, enabling effective early-generation selection. Anthocyanins are predominantly influenced by dominant effects, suitable for medium and high generation selection. All four antioxidant components display positive correlations among phenotype, genetics, additive and dominance effects, as well as additive × environment and dominance × environment interactions, with high correlation coefficients. Procyanidins exhibit a small genotype × environment interaction effect, indicating good stability. Using procyanidins as a selection indicator effectively enhances the other three components, reduces workload, and improves breeding efficiency. Higher temperatures contribute to increased accumulation of antioxidant components in red rice hybrid offspring, enhancing population heterosis. Elevating the content of antioxidant components in sterile lines benefits the cultivation of offspring with higher antioxidant content. Restorer lines 18Rr174 and 18Rr175 effectively increase the contents of total phenols, total flavonoids, and procyanidins in offspring and are less influenced by the environment. 【Conclusion】 Utilizing the additive-dominance genetic model for quantitative traits could effectively predict parental genetic effects on antioxidant components. 18Rr174 and 18Rr175 can be used to breed red rice with high antioxidant activity components.

Key words: red rice, antioxidant composition, genetic effect, genotype ×, environmental interaction effect