二化螟对甲氧虫酰肼的抗性风险、交互抗性及亚致死效应研究

doi:10.16819/j.1001-7216.2023.220804

摘要/Abstract

摘要：

【目的】 明确二化螟对甲氧虫酰肼的抗性风险、交互抗性及亚致死效应。【方法】 分别采用饲料混药法和稻苗浸渍法建立了二化螟初孵和2龄幼虫对甲氧虫酰肼的敏感基线，采用饲料混药法评估了二化螟对甲氧虫酰肼的抗性风险；采用稻苗浸渍法测定了抗甲氧虫酰肼二化螟田间种群2龄幼虫对抑食肼、虫酰肼和呋喃虫酰肼的交互抗性以及亚致死浓度抑食肼和甲氧虫酰肼对二化螟生长发育与繁殖的影响。【结果】 当抗性现实遗传力(h²)为0.402，斜率为2.451，平均选择压力分别为50%、60%、70%、80%和90%时，二化螟对甲氧虫酰肼的抗性上升10倍分别需要7.7、6.3、5.2、4.4和3.5代；2019年采自余姚的二化螟田间种群(YY-19)对甲氧虫酰肼处于中等水平抗性(RR = 73.4倍)，对呋喃虫酰肼(RR = 24.7倍)和虫酰肼(RR = 26.1倍)同样存在中等水平的交互抗性，对抑食肼(RR=2.9倍)有低水平交互抗性；采用亚致死浓度(LC₂₅)抑食肼和甲氧虫酰肼处理二化螟2龄幼虫后，幼虫历期显著增加，化蛹率和F₀代雌蛹重均显著降低。此外，F₀和F₁代的羽化率和单雌产卵量在经亚致死浓度甲氧虫酰肼处理后显著降低。【结论】 二化螟对甲氧虫酰肼具有较高的抗性风险。对甲氧虫酰肼产生抗性的田间二化螟种群与其他双酰肼类杀虫剂之间存在交互抗性。亚致死剂量甲氧虫酰肼处理二化螟具有显著的适合度代价。

关键词: 二化螟, 甲氧虫酰肼, 抗性风险评估, 交互抗性, 适合度代价

Abstract:

【Objective】 We aim to investigate the resistance risk, cross resistance, and sublethal effects of methoxyfenozide on Chilo suppressalis.【Method】 We established the susceptibility baseline of the rice stem borer to methoxyfenozide using rice seedling dipping method and diet incorporation method, respectively. We assessed its resistance risk using diet incorporation method and determined the cross resistance of methoxyfenozide-resistant C. suppressalis population to RH-5849, tebufenozide and fufenozide. Then, we evaluated the effects of sublethal concentration of RH-5849 and methoxyfenozide on the development and reproduction of C. suppressalis using rice seedling dipping method. 【Results】 The results showed that it took 7.7, 6.3, 5.2, 4.4, and 3.5 generations for C. suppressalis to acquire ten-fold resistance to methoxyfenozide when the realized heritability (h²) was 0.402, the slope was 2.451 and the average selective pressure was 50%, 60%, 70%, 80%, and 90%. The bioassay results showed that Yuyao-19 (YY-19) population with a moderate level of resistance to methoxyfenozide (RR=73.4) exhibited moderate cross resistance to fufenozide (RR=24.7) and tebufenozide (RR=26.1), and lower cross-resistance to RH-5849 (RR=2.9). The larval development duration significantly increased but the pupation rate and the pupal weight remarkably decreased after the treatment of sublethal dose of RH-5849 and methoxyfenozide to 2^nd instar larvae of C. suppressalis. Moreover, the eclosion rate and the egg laying number per female of F₀ and F₁ generations decreased significantly after the treatment of sublethal dose of methoxyfenozide. 【Conclusion】 C. suppressalis has a high resistance risk to methoxyfenozide. The field population which exhibits a moderate level of resistance to methoxyfenozide has cross-resistance with other diacylhydrazines. A significant fitness cost is present in rice stem borers treated with a sublethal dose of methoxyfenozide.

Key words: Chilo suppressalis, methoxyfenozide, resistance risk assessment, cross resistance, fitness cost

刘艳, 何林凤, 汪书超, 杨凤霞, 高聪芬, 吴顺凡. 二化螟对甲氧虫酰肼的抗性风险、交互抗性及亚致死效应研究[J]. 中国水稻科学, 2023, 37(4): 427-435.

LIU Yan, HE Linfeng, WANG Shuchao, YANG Fengxia, GAO Congfen, WU Shunfan. Resistance Risk, Cross Resistance and Sublethal Effects of Methoxyfenozide on Rice Stemborers (Chilo suppressalis)[J]. Chinese Journal OF Rice Science, 2023, 37(4): 427-435.

图/表 6

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种群 Population	生测方法 Bioassay methods	龄期 Instar	斜率±标准误 Slope ± SE	卡方值(自由度) χ²(df)	致死中浓度(95%置信限) LC₅₀(95%FL)/(mg·L⁻¹)
WH-S	稻苗浸渍法	1龄1st larval instar^a	1.718±0.219	1.212(4)	5.175(3.892−6.804)
	Seedling dipping method	2龄2nd larval instar^b	2.285±0.305	6.091(3)	9.903(4.470−18.392)
	人工饲料混药法 Artificial diet incorporation method	1龄1st larval instar^a	3.255±0.476	2.600(3)	0.213(0.171−0.257)
	人工饲料混药法 Artificial diet incorporation method	2龄2nd larval instar^b	2.686±0.335	2.697(3)	0.880(0.711−1.073)

种群 Population	生测方法 Bioassay methods	龄期 Instar	斜率±标准误 Slope ± SE	卡方值(自由度) χ²(df)	致死中浓度(95%置信限) LC₅₀(95%FL)/(mg·L⁻¹)
WH-S	稻苗浸渍法	1龄1st larval instar^a	1.718±0.219	1.212(4)	5.175(3.892−6.804)
	Seedling dipping method	2龄2nd larval instar^b	2.285±0.305	6.091(3)	9.903(4.470−18.392)
	人工饲料混药法 Artificial diet incorporation method	1龄1st larval instar^a	3.255±0.476	2.600(3)	0.213(0.171−0.257)
	人工饲料混药法 Artificial diet incorporation method	2龄2nd larval instar^b	2.686±0.335	2.697(3)	0.880(0.711−1.073)

筛选世代Generation	筛选浓度 Selection concentration/(mg·L⁻¹)	处理虫数 Number	平均存活率 Survival rate/%	斜率±标准误 Slope±SE	卡方值(自由度) χ² (df)	致死中浓度(95%置信限) LC₅₀(95%FL)/(mg·L⁻¹)	RR
M₀	0.3	10 000	60	2.511±0.327	1.082(3)	0.424(0.336−0.517)	1.0
M₁	0.3	4 000	70	2.934±0.377	2.320(3)	0.761(0.636−0.929)	1.8
M₂	0.3	5 000	83	2.942±0.407	2.417(4)	0.470(0.369−0.584)	1.0
M₃	0.4	6 000	68	1.989±0.231	7.507(4)	0.596(0.332−0.924)	1.4
M₄	0.2	650	95	1.994±0.279	0.536(3)	1.598(1.258−2.163)	3.8
M₅	0.3	4 000	73	2.723±0.288	1.212(4)	1.393(1.162−1.674)	3.3
M₆	0.8	14 000	73	2.464±0.299	5.829(3)	0.711(0.417−1.182)	1.7
M₇	1.6	14 000	52	2.608±0.361	1.936(4)	1.510(1.170−1.876)	3.6
M₈	0.4	500	60	−	−	−	−
M₉	0.8	3 000	87	2.141±0.242	5.932(4)	1.428(0.935−2.067)	3.7
M₁₀	0.8	5 000	88	2.673±0.326	3.647(4)	2.253(1.793−2.770)	5.3

筛选世代Generation	筛选浓度 Selection concentration/(mg·L⁻¹)	处理虫数 Number	平均存活率 Survival rate/%	斜率±标准误 Slope±SE	卡方值(自由度) χ² (df)	致死中浓度(95%置信限) LC₅₀(95%FL)/(mg·L⁻¹)	RR
M₀	0.3	10 000	60	2.511±0.327	1.082(3)	0.424(0.336−0.517)	1.0
M₁	0.3	4 000	70	2.934±0.377	2.320(3)	0.761(0.636−0.929)	1.8
M₂	0.3	5 000	83	2.942±0.407	2.417(4)	0.470(0.369−0.584)	1.0
M₃	0.4	6 000	68	1.989±0.231	7.507(4)	0.596(0.332−0.924)	1.4
M₄	0.2	650	95	1.994±0.279	0.536(3)	1.598(1.258−2.163)	3.8
M₅	0.3	4 000	73	2.723±0.288	1.212(4)	1.393(1.162−1.674)	3.3
M₆	0.8	14 000	73	2.464±0.299	5.829(3)	0.711(0.417−1.182)	1.7
M₇	1.6	14 000	52	2.608±0.361	1.936(4)	1.510(1.170−1.876)	3.6
M₈	0.4	500	60	−	−	−	−
M₉	0.8	3 000	87	2.141±0.242	5.932(4)	1.428(0.935−2.067)	3.7
M₁₀	0.8	5 000	88	2.673±0.326	3.647(4)	2.253(1.793−2.770)	5.3

筛选代数 Selected generation	每代平均反应 Estimate of mean response per generation				每代平均选择差异 Estimate of mean selected differential per generation					现实遗传力h²
筛选代数 Selected generation	始LC₅₀对数值 Logarithm of initial LC₅₀	终LC₅₀对数值 Logarithm of final LC₅₀	选择反应 R		平均存活率 P	选择强度 i	平均斜率 Mean slope	表型标准差 σ_p	选择差异 S
10(M₁~M₁₀)	−0.373	0.353	0.073		73.55	0.442	2.451	0.408	0.180	0.402