中国水稻科学 ›› 2020, Vol. 34 ›› Issue (6): 586-594.DOI: 10.16819/j.1001-7216.2020.0101

• • 上一篇    

氯虫苯甲酰胺胁迫下二化螟中肠细菌类微生物的多样性

张珏锋1, 张琴2, 李芳1, 钟海英1, 陈建明1,*()   

  1. 1浙江省农业科学院植物保护与微生物研究所,杭州 310021
    2杭州市林业科学研究院,杭州 310021
  • 收稿日期:2020-01-06 修回日期:2020-04-20 出版日期:2020-11-10 发布日期:2020-11-10
  • 通讯作者: 陈建明
  • 基金资助:
    浙江省公益科技计划资助项目 (2016C32100);现代农业产业技术体系专项(CARS-24-G-06)

Diversity of Midgut Microbial Community of ChilosuppressalisExposed to Chlorobenzamide

Juefeng ZHANG1, Qin ZHANG2, Fang LI1, Haiying ZHONG1, Jianming CHEN1,*()   

  1. 1Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences, Hangzhou310021, China
    2Hangzhou Academy of forestry,Hangzhou 310021, China
  • Received:2020-01-06 Revised:2020-04-20 Online:2020-11-10 Published:2020-11-10
  • Contact: Jianming CHEN

摘要: 目的 明确氯虫苯甲酰胺处理下二化螟中肠细菌类微生物多样性的变化。方法 综合运用宏基因组测序以及传统微生物分离纯化的方法分析不同浓度氯虫苯甲酰胺处理的二化螟种群中肠微生物的多样性。结果 不同浓度(100、200、400µg/mL)氯虫苯甲酰胺处理后二化螟中肠细菌类微生物丰度及多样性降低,处理种群中肠细菌类微生物的OTU数值、特有的OTU数值均低于对照;摩根氏菌属(Morganella)、普罗威登斯菌属(Providencia)以及变形菌属(Proteus)在处理种群中的比例明显升高;同时培养结果显示氯虫苯甲酰胺处理种群获得的菌株除肠杆菌属(Enterobacter)外,还存在超压莱略特氏菌(Lelliottianimipressuralis)、非脱羧勒菌(Leclerciaadecarboxylata)、成团泛菌(Pantoeaagglomerans)等其他菌属。COG数据库对比与KEGG分析显示,对照与处理二化螟种群的各功能分类基因数量及相对丰度无明显差异。结论 解析了氯虫苯甲酰胺胁迫下二化螟中肠细菌类微生物的多样性。这些结果为进一步研究中肠细菌在二化螟对氯虫苯甲酰胺的抗药性奠定基础。

关键词: 二化螟, 氯虫苯甲酰胺:宏基因组, 中肠细菌, 微生物多样性

Abstract:

【Objective】We aim toilluminatethestructural changes in the midgut microbial community of Chilosuppressalis exposed to chlorobenzamide.【Method】Macrogenomic sequencing and in vitro culturewere used to analyze the diversity of midgut microbiotaof Chilosuppressalistreatedwith different concentrations (100, 200, 400µg/mL) of chlorobenzamide.【Result】The results showed that chlorobenzamide treatmentdecreased the abundance anddiversityof the midgut bacteria of Chilosuppressalis,and the OTU number and specific OTU number of the intestinal bacteria of treated population were lower than those of the control;the proportion of Morganella, Provincia and Proteus in the chlorobenzamide treated population increased significantly. Meanwhile, the result of in vitro culture indicated besides enterobacter,Lelliottinimipressuralis, Leclerciadecarboxylata, Pantoeaagglomeranswere isolated from chlorobenzamide treated Chilosuppressalis population. COG classification and KEGG analysis didn’t show significant difference in number and relative abundance of functional genes among control and three chlorobenzamide treated populations.【Conclusion】The study had clarified the diversity of intestinal bacteria and the relationshipbetween gutbacteria of Chilosuppressalis andthe formation of chlorobenzamide resistance, and will lay a scientific basis for the role of intestinal bacteria in resistance of Chilosuppressalistochlorobenzamide.

Key words: Chilosuppressalis, chlorobenzamide, macrogenomic sequencing, midgut bacteria, microbial diversity

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