Analysis on Gene Locus of Magnaporthe oryzae B11, a PathogenicityEnhanced Mutant
WU Xiao-yan1, 2, WANG Jiao-yu2,*, ZHANG Zhen2, JING Jin-xue1, DU Xin-fa2, CHAI Rong-yao2, MAO Xue-qin2, QIU Hai-ping2, JIANG Hua2, WANG Yan-li2, SUN Guo-chang2,*
1 Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2College of Plant Protection, Northwest Agricultural and Forest University, Yangling 712100, China; *Corresponding author, E-mail: jiaoyuwang1@gmail.com；sungc01@sina.com
Abstract：
A Magnaporthe oryzae mutant B11 was obtained by Agrobacteriummediated transformation (AtMT) method, which enhanced the pathogenicity to barley. Southern blotting analysis indicated that TDNA insert in the B11 genome was single copy. TAILPCR and sequence alignment analysis indicated that the TDNA insertion broke the putative gene locus MG01679. By using PCRbased method, the DNA and cDNA of the gene locus were cloned and sequenced. The open reading frame of the gene includes one intron and two exons. And the coding sequence is 696 bp long and encodes a 231 amino acid peptide. Protein similarity analysis indicated that the product of the gene belongs to the ThiJ/PfpⅠ protein family, and the gene was thus assigned MgThiJ1. MgThiJ1 shows 57% similarity to FOXG_09029 from Fusarium oxysporum, and 54% similarity to FGSG_08979 from Fusarium graminearum in protein sequence. MgThiJ1 gene may be a negative regulator to vegetative growth and pathogenicity in filamentous fungi, and its specific mechanism need to be studied further.
WU Xiao-yan,WANG Jiao-yu,ZHANG Zhen et al. Analysis on Gene Locus of Magnaporthe oryzae B11, a PathogenicityEnhanced Mutant [J]. , 2009, 23(6): 611-615 .
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2009, Vol. 23 
