中国水稻科学 ›› 2023, Vol. 37 ›› Issue (5): 543-552.DOI: 10.16819/j.1001-7216.2023.230201
• 研究报告 • 上一篇
夏杨1, 李传明2, 刘琴1,2, 韩光杰1, 徐彬1, 黄立鑫1, 祁建杭2, 陆玉荣1, 徐健1,*()
收稿日期:
2023-02-01
修回日期:
2023-05-11
出版日期:
2023-09-10
发布日期:
2023-09-13
通讯作者:
*email: 基金资助:
XIA Yang1, LI Chuanming2, LIU Qin1,2, HAN Guangjie1, XU Bin1, HUANG Lixin1, QI Jianhang2, LU Yurong1, XU Jian1,*()
Received:
2023-02-01
Revised:
2023-05-11
Online:
2023-09-10
Published:
2023-09-13
Contact:
*email: 摘要:
【目的】 探究印度梨形孢(Piriformospora indica)PI-020对盐胁迫下水稻幼苗生长、抗氧化相关酶活性及基因表达水平的影响。【方法】 以不同浓度的PI-020菌丝体悬液接种南粳9108水培苗,显微镜观察及qPCR技术分析PI-020在水稻秧苗根系的定殖能力,并测定水稻叶片丙二醛(MDA)含量,分析水稻秧苗的表型参数、光合色素含量、抗氧化相关酶活性及基因表达的变化。【结果】 与对照相比,盐胁迫条件下(100 mmol/L NaCl),PI-020定殖后,水稻叶片MDA含量显著降低。200倍菌丝体稀释液处理的效果最好,MDA含量减少了67.2%。PI-020定殖后,水稻株高、根长、叶面积、鲜质量和干质量分别增加了34.67%、23.62%、58.04%、59.53%和67.25%,与对照相比均差异显著;同时PI-020定殖还显著提高了叶片光合色素含量,抗氧化酶CAT、APX、POD活性及抗氧化相关基因OsCAT、OsAPX2、OsSOD的表达水平。【结论】 印度梨形孢PI-020通过提高水稻幼苗抗氧化能力减少盐胁迫引起的氧化损伤,从而降低MDA含量,同时缓解光合色素的降解,保护了水稻光合系统,进而提高水稻耐盐性。
夏杨, 李传明, 刘琴, 韩光杰, 徐彬, 黄立鑫, 祁建杭, 陆玉荣, 徐健. 印度梨形孢对盐胁迫下水稻幼苗生长及抗氧化系统的影响[J]. 中国水稻科学, 2023, 37(5): 543-552.
XIA Yang, LI Chuanming, LIU Qin, HAN Guangjie, XU Bin, HUANG Lixin, QI Jianhang, LU Yurong, XU Jian. Effects of Piriformospora indica on the Growth and Antioxidant System of Rice Seedlings Under Salt Stress[J]. Chinese Journal OF Rice Science, 2023, 37(5): 543-552.
基因名称 Gene name | 正向引物序列 Forward primer sequence (5'-3') | 反向引物序列 Reverse primer sequence (5'-3') |
---|---|---|
OsUBQ5 | ACCACTTCGACCGCCACTACT | ACGCCTAAGCCTGCTGGTT |
OsCAT | ATCATCGTGCCGGGGATCTA | AAGCCGTCGTAGTGGTTGTT |
OsAPX2 | CTTCGGCACCATGAAGAACC | CTGGTAGAAGTCGGCGTAGG |
OsSOD | CCGTGTGACGGGACTTACTC | GGTTGCCTCAGCTACACCTT |
OsGR2 | ATTGAAGGGGCAGGCAGTTT | TCCGCCACCAAGGATTACAG |
表1 qRT-PCR所用引物序列
Table 1. Primer sequences for qRT-PCR.
基因名称 Gene name | 正向引物序列 Forward primer sequence (5'-3') | 反向引物序列 Reverse primer sequence (5'-3') |
---|---|---|
OsUBQ5 | ACCACTTCGACCGCCACTACT | ACGCCTAAGCCTGCTGGTT |
OsCAT | ATCATCGTGCCGGGGATCTA | AAGCCGTCGTAGTGGTTGTT |
OsAPX2 | CTTCGGCACCATGAAGAACC | CTGGTAGAAGTCGGCGTAGG |
OsSOD | CCGTGTGACGGGACTTACTC | GGTTGCCTCAGCTACACCTT |
OsGR2 | ATTGAAGGGGCAGGCAGTTT | TCCGCCACCAAGGATTACAG |
图1 PI-020在水稻苗根系的定殖情况 A-显微镜观察;B-定殖量测定。PI-020为接种处理;CK为未接种处理。
Fig. 1. Colonization of PI-020 in rice roots. A, Microscopic observation; B, Determination of PI-020 colonization. PI-020, Inoculation with PI-020 treatment; CK, Uninoculation treatment.
图2 PI-020定殖对水稻盐胁迫耐受能力的影响 A-水稻盐胁迫7 d后的表型,标尺为3 cm;B-水稻盐胁迫7 d后叶片丙二醛含量。CK为对照;N为盐胁迫;P1N为50倍梨形孢稀释液+盐胁迫;P2N为100倍梨形孢稀释液+盐胁迫;P3N为200倍梨形孢稀释液+盐胁迫;P4N为400倍梨形孢稀释液+盐胁迫;P5N为800倍梨形孢稀释液+盐胁迫。不同小写字母表示处理之间差异达0.05显著水平。下同。
Fig. 2. Effects of PI-020 colonization on salt tolerance of rice. A, Phenotypes of rice seedlings after 7 days of salt stress, Bar=3 cm; B, MDA contents in rice leaves after 7 days of salt stress. N, NaCl; P1N, P1+NaCl; P2N, P2+NaCl; P3N, P3+NaCl; P4N, P4+NaCl; P5N, P5+NaCl. Different lowercase letters indicate significant differences (P < 0.05). The same below.
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