中国水稻科学 ›› 2020, Vol. 34 ›› Issue (4): 339-347.DOI: 10.16819/j.1001-7216.2020.9102
徐飞1,2, 隋文志1,*(), 张拓2, 怀宝东1, 杨雪1,2
收稿日期:
2019-09-19
修回日期:
2020-04-09
出版日期:
2020-07-10
发布日期:
2020-07-10
通讯作者:
隋文志
基金资助:
Fei XU1,2, Wenzhi SUI1,*(), Tuo ZHANG2, Baodong HUAI1, Xue YANG1,2
Received:
2019-09-19
Revised:
2020-04-09
Online:
2020-07-10
Published:
2020-07-10
Contact:
Wenzhi SUI
摘要:
【目的】旨在通过优化水稻生育进程的水肥供给来促进水稻的生长发育。【方法】大田试验于2016—2017年在黑龙江省八五二农场进行,以垦稻24 (主茎叶片数为12) 为材料,采用2种灌溉模式和2种施肥模式的正交试验设计:叶龄灌溉模式 (LI) 与常规灌溉模式 (RI),叶龄调控侧深施肥模式 (SF) 与常规施肥模式 (RF)。【结果】LI+SF模式对水稻形态特征、光合特性、水肥利用效率及产量具有显著影响(P<0.05)。与RI+RF相比,LI+SF模式使叶面积指数、单株不定根数、总根长、总根表面积及总体积显著提高了21.2%、22.7%、20.8%、45.2%和17.8%,灌浆期水稻光合速率、净光合速率、叶绿素含量及气孔导度显著提高了17.4%、18.4%、26.8%和40.1%,水稻结实率、千粒重和理论产量显著提高了6.3%、2.4%和28.2%,实收产量平均增加5.2%,纯收入增加13.2%,氮肥农学利用率、籽粒吸氮量、产谷利用效率、氮肥偏生产力及氮收获指数被显著提高了11.1%、6.9%、5.8%、13.6%和8.0%。LI模式可以节约灌溉水用量1 000 t/hm2。RI+SF对水稻植株形态、光合特性、生物学产量和水肥利用率具有一定的促进作用,实收产量平均增加2.3%,没有充分发挥水肥耦合作用。【结论】LI+SF模式为本研究最佳的精准灌溉施肥模式,也是适合在我国北方推广的寒地水稻节水、节肥、丰产技术模式。
中图分类号:
徐飞, 隋文志, 张拓, 怀宝东, 杨雪. 叶龄调控下水肥耦合对寒地水稻生物学特征及水肥利用效率的影响[J]. 中国水稻科学, 2020, 34(4): 339-347.
Fei XU, Wenzhi SUI, Tuo ZHANG, Baodong HUAI, Xue YANG. Effects of Irrigation and Fertilization Coupling on Biological Characteristics, Water and Nitrogen Use Efficiencies of Rice in Cold Regions Under Leaf Age Regulation[J]. Chinese Journal OF Rice Science, 2020, 34(4): 339-347.
年份 Year | 处理 Treatment | 株高 Plant height/cm | 分蘖数 Tiller number | 叶面积指数 Leaf area index | 不定根数Indefinite root number | 总根长 Total root length/m | 总根表面积 Total root surface area/(×102 cm2·plant-1) | 总根体积 Total root volume /(cm3·plant-1) |
---|---|---|---|---|---|---|---|---|
2016 | RI+RF | 68.42±4.39 a | 4.53±0.75 a | 3.90±0.24 b | 291.22±8.76 b | 42.48±2.11 b | 13.15±2.22 b | 80.44±4.43 b |
LI+RF | 67.53±5.17 a | 4.80±0.92 a | 4.17±0.16 b | 357.56±18.58 a | 47.90±2.32 ab | 18.56±2.11 a | 88.32±5.52 ab | |
RI+SF | 68.75±4.63 a | 5.63±1.05 a | 3.93±0.34 b | 312.54±13.67 b | 45.53±2.14 b | 15.32±1.05 b | 92.74±4.35 ab | |
LI+SF | 65.95±5.23 a | 5.52±1.03 a | 4.83±0.23 a | 363.51±24.79 a | 50.73±3.63 a | 19.11±3.23 a | 95.89±4.32 a | |
2017 | RI+RF | 68.04±5.06 a | 4.79±0.86 a | 3.93±0.15 b | 288.67±9.70 b | 43.38±4.35 b | 14.08±2.03 b | 81.24±3.63 b |
LI+RF | 67.46±5.64 a | 4.85±1.05 a | 3.83±0.24 b | 339.83±12.14 a | 49.23±2.14 ab | 19.15±3.02 a | 86.13±5.48 ab | |
RI+SF | 69.31±4.95 a | 5.42±0.94 a | 4.16±0.23 b | 310.59±12.17 b | 46.46±2.26 b | 18.89±1.86 ab | 90.42±2.85 ab | |
LI+SF | 68.31±5.92 a | 5.58±1.03 a | 4.66±0.25 a | 348.24±18.76 a | 52.98±3.28 a | 20.43±3.28 a | 94.56±5.46 a |
表1 不同灌溉施肥模式水稻形态特征的差异
Table 1 Difference in morphological traits under different irrigation and fertilization modes.
年份 Year | 处理 Treatment | 株高 Plant height/cm | 分蘖数 Tiller number | 叶面积指数 Leaf area index | 不定根数Indefinite root number | 总根长 Total root length/m | 总根表面积 Total root surface area/(×102 cm2·plant-1) | 总根体积 Total root volume /(cm3·plant-1) |
---|---|---|---|---|---|---|---|---|
2016 | RI+RF | 68.42±4.39 a | 4.53±0.75 a | 3.90±0.24 b | 291.22±8.76 b | 42.48±2.11 b | 13.15±2.22 b | 80.44±4.43 b |
LI+RF | 67.53±5.17 a | 4.80±0.92 a | 4.17±0.16 b | 357.56±18.58 a | 47.90±2.32 ab | 18.56±2.11 a | 88.32±5.52 ab | |
RI+SF | 68.75±4.63 a | 5.63±1.05 a | 3.93±0.34 b | 312.54±13.67 b | 45.53±2.14 b | 15.32±1.05 b | 92.74±4.35 ab | |
LI+SF | 65.95±5.23 a | 5.52±1.03 a | 4.83±0.23 a | 363.51±24.79 a | 50.73±3.63 a | 19.11±3.23 a | 95.89±4.32 a | |
2017 | RI+RF | 68.04±5.06 a | 4.79±0.86 a | 3.93±0.15 b | 288.67±9.70 b | 43.38±4.35 b | 14.08±2.03 b | 81.24±3.63 b |
LI+RF | 67.46±5.64 a | 4.85±1.05 a | 3.83±0.24 b | 339.83±12.14 a | 49.23±2.14 ab | 19.15±3.02 a | 86.13±5.48 ab | |
RI+SF | 69.31±4.95 a | 5.42±0.94 a | 4.16±0.23 b | 310.59±12.17 b | 46.46±2.26 b | 18.89±1.86 ab | 90.42±2.85 ab | |
LI+SF | 68.31±5.92 a | 5.58±1.03 a | 4.66±0.25 a | 348.24±18.76 a | 52.98±3.28 a | 20.43±3.28 a | 94.56±5.46 a |
年份 Year | 处理 Treatment | 光合速率 | 净光合速率 | 叶绿素含量 | 胞间CO2浓度 | 气孔导度 |
---|---|---|---|---|---|---|
Photosynthetic rate | Net photosynthetic rate | Chlorophyll contents | CO2 concentration | Stomatal conductance | ||
/(μmol·m-2 s-1) | /(μmol·m-2 s-1) | /(mg·m-2) | /(μmol·mol-1) | /(mol·m-2 s-1) | ||
2016 | RI+RF | 21.31±1.54 b | 21.11±1.06 b | 203.45±13.66 b | 247.17±19.83 a | 0.81±0.13 b |
LI+RF | 21.51±1.45 b | 21.34±0.96 b | 208.14±16.51 b | 249.28±15.44 a | 0.97±0.07 b | |
RI+SF | 24.38±1.36 a | 24.23±1.64 a | 251.89±20.24 a | 245.21±9.89 a | 1.08±0.11 ab | |
LI+SF | 25.24±1.87 a | 25.03±1.39 a | 271.64±24.61 a | 226.11±15.35 a | 1.21±0.07 a | |
2017 | RI+RF | 21.62±1.57 b | 21.12±0.97 c | 214.37±12.56 c | 254.49±16.88 a | 1.01±0.11 c |
LI+RF | 21.15±1.78 b | 21.93±1.65 b | 219.27±13.56 b | 245.09±18.64 a | 1.11±0.13 ab | |
RI+SF | 24.95±1.67 a | 24.66±1.85 a | 256.61±18.06 a | 225.12±10.07 a | 1.14±0.17 ab | |
LI+SF | 25.15±1.37 a | 24.98±1.94 a | 257.53±14.66 a | 232.58±19.31 a | 1.32±0.09 a |
表2 不同灌溉施肥模式下水稻光合特性的差异
Table 2 Difference in photosynthetic characteristics under different irrigation and fertilization modes.
年份 Year | 处理 Treatment | 光合速率 | 净光合速率 | 叶绿素含量 | 胞间CO2浓度 | 气孔导度 |
---|---|---|---|---|---|---|
Photosynthetic rate | Net photosynthetic rate | Chlorophyll contents | CO2 concentration | Stomatal conductance | ||
/(μmol·m-2 s-1) | /(μmol·m-2 s-1) | /(mg·m-2) | /(μmol·mol-1) | /(mol·m-2 s-1) | ||
2016 | RI+RF | 21.31±1.54 b | 21.11±1.06 b | 203.45±13.66 b | 247.17±19.83 a | 0.81±0.13 b |
LI+RF | 21.51±1.45 b | 21.34±0.96 b | 208.14±16.51 b | 249.28±15.44 a | 0.97±0.07 b | |
RI+SF | 24.38±1.36 a | 24.23±1.64 a | 251.89±20.24 a | 245.21±9.89 a | 1.08±0.11 ab | |
LI+SF | 25.24±1.87 a | 25.03±1.39 a | 271.64±24.61 a | 226.11±15.35 a | 1.21±0.07 a | |
2017 | RI+RF | 21.62±1.57 b | 21.12±0.97 c | 214.37±12.56 c | 254.49±16.88 a | 1.01±0.11 c |
LI+RF | 21.15±1.78 b | 21.93±1.65 b | 219.27±13.56 b | 245.09±18.64 a | 1.11±0.13 ab | |
RI+SF | 24.95±1.67 a | 24.66±1.85 a | 256.61±18.06 a | 225.12±10.07 a | 1.14±0.17 ab | |
LI+SF | 25.15±1.37 a | 24.98±1.94 a | 257.53±14.66 a | 232.58±19.31 a | 1.32±0.09 a |
年份 Year | 处理 Treatment | 穗数 Number of panicle per m2 | 每穗粒数 Grain number per panicle | 结实率 Seed-setting rate /% | 千粒重 Thousand-grain weight /g | 理论产量 Theoretical yield /(t·hm-2) | 实收产量 Real yield /(t·hm-2) |
---|---|---|---|---|---|---|---|
2016 | RI+RF | 486.51±18.96 a | 108.51±2.77 a | 84.13±1.21 b | 22.33±0.19 b | 9.92±0.31 b | 8.89 |
LI+RF | 484.84±15.78 a | 112.56±2.43 a | 84.17±1.15 b | 22.25±0.26 b | 10.22±0.19 b | 8.92 | |
RI+SF | 508.75±21.6 a | 116.87±3.62 a | 86.16±1.42 b | 22.71±0.23 ab | 11.63±0.73 a | 9.01 | |
LI+SF | 524.79±33.78 a | 117.51±4.58 a | 89.12±1.36 a | 22.78±0.19 a | 12.54±1.25 a | 9.37 | |
2017 | RI+RF | 501.99±20.52 a | 106.79±3.43 a | 84.56±1.22 b | 22.07±0.18 b | 10.00±1.25 b | 9.06 |
LI+RF | 506.08±21.79 a | 111.38±1.69 a | 85.39±1.51 b | 22.16±0.24 ab | 10.67±0.49 b | 9.12 | |
RI+SF | 527.57±18.04 a | 115.68±3.65 a | 86.77±1.09 b | 22.72±0.29 a | 12.03±0.66 a | 9.35 | |
LI+SF | 536.22±21.07 a | 118.24±3.59 a | 90.27±1.36 a | 22.69±0.36 a | 12.99±1.64 a | 9.53 |
表3 不同灌溉施肥模式下水稻产量的差异
Table 3 Difference in rice yield under different irrigation and fertilization modes.
年份 Year | 处理 Treatment | 穗数 Number of panicle per m2 | 每穗粒数 Grain number per panicle | 结实率 Seed-setting rate /% | 千粒重 Thousand-grain weight /g | 理论产量 Theoretical yield /(t·hm-2) | 实收产量 Real yield /(t·hm-2) |
---|---|---|---|---|---|---|---|
2016 | RI+RF | 486.51±18.96 a | 108.51±2.77 a | 84.13±1.21 b | 22.33±0.19 b | 9.92±0.31 b | 8.89 |
LI+RF | 484.84±15.78 a | 112.56±2.43 a | 84.17±1.15 b | 22.25±0.26 b | 10.22±0.19 b | 8.92 | |
RI+SF | 508.75±21.6 a | 116.87±3.62 a | 86.16±1.42 b | 22.71±0.23 ab | 11.63±0.73 a | 9.01 | |
LI+SF | 524.79±33.78 a | 117.51±4.58 a | 89.12±1.36 a | 22.78±0.19 a | 12.54±1.25 a | 9.37 | |
2017 | RI+RF | 501.99±20.52 a | 106.79±3.43 a | 84.56±1.22 b | 22.07±0.18 b | 10.00±1.25 b | 9.06 |
LI+RF | 506.08±21.79 a | 111.38±1.69 a | 85.39±1.51 b | 22.16±0.24 ab | 10.67±0.49 b | 9.12 | |
RI+SF | 527.57±18.04 a | 115.68±3.65 a | 86.77±1.09 b | 22.72±0.29 a | 12.03±0.66 a | 9.35 | |
LI+SF | 536.22±21.07 a | 118.24±3.59 a | 90.27±1.36 a | 22.69±0.36 a | 12.99±1.64 a | 9.53 |
年份 Year | 处理 Treatment | 水分利用率 Efficiency of water application /(kg·m-3) | 氮肥农学利用效率 Efficiency of fertilizer use /(kg·kg-1) | 籽粒吸收氮量 N uptake of grains /(kg·hm-2) | 产谷利用效率 IEN /(kg·kg-1) | 氮肥偏生产力 PFPN /(kg·kg-1) | 氮收获指数 HIN /% |
---|---|---|---|---|---|---|---|
2016 | RI+RF | 0.99±0.02 b | 19.83±0.56 b | 76.62±2.46 b | 63.23±2.04 b | 70.96±5.31 b | 61.52±1.66 b |
LI+RF | 1.12±0.07 a | 20.11±0.51 b | 76.18±3.48 b | 65.48±1.23 b | 75.33±3.54 b | 60.46±1.74 b | |
RI+SF | 1.00±0.03 b | 21.34±0.47 a | 79.66±2.54 a | 66.63±0.78 a | 79.12±2.47 a | 66.43±2.26 a | |
LI+SF | 1.17±0.06 a | 21.54±0.36 a | 81.78±2.93 a | 68.55±0.78 a | 80.80±2.87 a | 67.32±3.42 a | |
2017 | RI+RF | 1.01±0.06 b | 19.01±0.98 b | 75.16±3.47 b | 63.27±1.22 b | 69.36±4.53 b | 60.67±1.47 b |
LI+RF | 1.14±0.06 a | 20.42±0.38 b | 76.08±2.37 b | 64.75±0.96 b | 75.46±2.67 b | 59.31±0.97 b | |
RI+SF | 1.04±0.02 b | 21.77±0.64 a | 82.49±2.11 a | 67.26±1.27 a | 80.23±2.26 a | 65.54±3.64 a | |
LI+SF | 1.19±0.05 a | 21.57±0.28 a | 85.66±2.37 a | 67.35±0.79 a | 80.91±3.68 a | 66.43±2.42 a |
表4 不同灌溉施肥模式水稻水分与氮肥利用效率的差异
Table 4 Difference in water and N fertilizer use efficiency under different irrigation and fertilization modes.
年份 Year | 处理 Treatment | 水分利用率 Efficiency of water application /(kg·m-3) | 氮肥农学利用效率 Efficiency of fertilizer use /(kg·kg-1) | 籽粒吸收氮量 N uptake of grains /(kg·hm-2) | 产谷利用效率 IEN /(kg·kg-1) | 氮肥偏生产力 PFPN /(kg·kg-1) | 氮收获指数 HIN /% |
---|---|---|---|---|---|---|---|
2016 | RI+RF | 0.99±0.02 b | 19.83±0.56 b | 76.62±2.46 b | 63.23±2.04 b | 70.96±5.31 b | 61.52±1.66 b |
LI+RF | 1.12±0.07 a | 20.11±0.51 b | 76.18±3.48 b | 65.48±1.23 b | 75.33±3.54 b | 60.46±1.74 b | |
RI+SF | 1.00±0.03 b | 21.34±0.47 a | 79.66±2.54 a | 66.63±0.78 a | 79.12±2.47 a | 66.43±2.26 a | |
LI+SF | 1.17±0.06 a | 21.54±0.36 a | 81.78±2.93 a | 68.55±0.78 a | 80.80±2.87 a | 67.32±3.42 a | |
2017 | RI+RF | 1.01±0.06 b | 19.01±0.98 b | 75.16±3.47 b | 63.27±1.22 b | 69.36±4.53 b | 60.67±1.47 b |
LI+RF | 1.14±0.06 a | 20.42±0.38 b | 76.08±2.37 b | 64.75±0.96 b | 75.46±2.67 b | 59.31±0.97 b | |
RI+SF | 1.04±0.02 b | 21.77±0.64 a | 82.49±2.11 a | 67.26±1.27 a | 80.23±2.26 a | 65.54±3.64 a | |
LI+SF | 1.19±0.05 a | 21.57±0.28 a | 85.66±2.37 a | 67.35±0.79 a | 80.91±3.68 a | 66.43±2.42 a |
水稻产量 Rice yield | 株高 Plant height | 分蘖数 Tiller number | 叶面积指数 Leaf area index | 不定根数 Indefinite root number | 总根长 Total root length | 总根表面积 Total root surface area | 总根体积 Total root volume |
---|---|---|---|---|---|---|---|
穗数Number of panicle | 0.547 | 0.302 | 0.596 | 0.488 | 0.421 | 0.246 | 0.285 |
每穗粒数Grain number per panicle | 0.410 | 0.531 | 0.359 | 0.299 | 0.169 | 0.500 | 0.459 |
结实率Seed setting rate | 0.321 | 0.466 | 0.798* | 0.849** | 0.901** | 0.788* | 0.654* |
千粒重Thousand-grain weight | 0.511 | 0.423 | 0.661* | 0.739* | 0.574 | 0.854** | 0.621* |
理论产量Theoretical yield | 0.363 | 0.511 | 0.784* | 0.895** | 0.912** | 0.831** | 0.796* |
表5 水稻产量指标与形态特征的相关系数
Table 5 Correlation coefficients (r) between water use efficiency and rice yield index.
水稻产量 Rice yield | 株高 Plant height | 分蘖数 Tiller number | 叶面积指数 Leaf area index | 不定根数 Indefinite root number | 总根长 Total root length | 总根表面积 Total root surface area | 总根体积 Total root volume |
---|---|---|---|---|---|---|---|
穗数Number of panicle | 0.547 | 0.302 | 0.596 | 0.488 | 0.421 | 0.246 | 0.285 |
每穗粒数Grain number per panicle | 0.410 | 0.531 | 0.359 | 0.299 | 0.169 | 0.500 | 0.459 |
结实率Seed setting rate | 0.321 | 0.466 | 0.798* | 0.849** | 0.901** | 0.788* | 0.654* |
千粒重Thousand-grain weight | 0.511 | 0.423 | 0.661* | 0.739* | 0.574 | 0.854** | 0.621* |
理论产量Theoretical yield | 0.363 | 0.511 | 0.784* | 0.895** | 0.912** | 0.831** | 0.796* |
水稻产量指标 Rice yield index | 水分利用率 EWA | 氮肥利用效率 EFU | 籽粒吸收氮量 N uptake of grains | 产谷利用效率 IEN | 氮肥偏生产力 PFPN | 氮收获指数 HIN |
---|---|---|---|---|---|---|
穗数Number of panicle | 0.565 | 0.531 | 0.404 | 0.555 | 0.285 | 0.469 |
每穗粒数Grain number per panicle | 0.458 | 0.354 | 0.296 | 0.497 | 0.157 | 0.302 |
结实率Seed setting rate | 0.853** | 0.828** | 0.789* | 0.809** | 0.833** | 0.845** |
千粒重Thousand-grain weight | 0.641* | 0.977** | 0.824** | 0.833** | 0.904** | 0.751* |
理论产量Theoretical yield | 0.868** | 0.874** | 0.882** | 0.797* | 0.836** | 0.799* |
表6 水稻产量指标与水、氮肥利用效率的相关系数
Table 6 Correlation coefficients (r) between rice yield index and use efficiencies of water and N.
水稻产量指标 Rice yield index | 水分利用率 EWA | 氮肥利用效率 EFU | 籽粒吸收氮量 N uptake of grains | 产谷利用效率 IEN | 氮肥偏生产力 PFPN | 氮收获指数 HIN |
---|---|---|---|---|---|---|
穗数Number of panicle | 0.565 | 0.531 | 0.404 | 0.555 | 0.285 | 0.469 |
每穗粒数Grain number per panicle | 0.458 | 0.354 | 0.296 | 0.497 | 0.157 | 0.302 |
结实率Seed setting rate | 0.853** | 0.828** | 0.789* | 0.809** | 0.833** | 0.845** |
千粒重Thousand-grain weight | 0.641* | 0.977** | 0.824** | 0.833** | 0.904** | 0.751* |
理论产量Theoretical yield | 0.868** | 0.874** | 0.882** | 0.797* | 0.836** | 0.799* |
年份 Year | 处理 Treatment | 单位产值 Output value | 灌溉水成本 Irrigation water cost | 人工成本 Labor cost | 总成本 Total cost | 纯收入 Net income |
---|---|---|---|---|---|---|
2016 | RI+RF | 24 536.4 | 225 | 9 000 | 18 630 | 5906.4 |
LI+RF | 24 619.2 | 225 | 9 400 | 19 030 | 5589.2 | |
RI+SF | 24 867.6 | 225 | 9 100 | 18 730 | 6137.6 | |
LI+SF | 25 861.2 | 225 | 9 500 | 19 130 | 6731.2 | |
2017 | RI+RF | 25 005.6 | 225 | 9 000 | 18 630 | 6375.6 |
LI+RF | 25 171.2 | 225 | 9 400 | 19 030 | 6141.2 | |
RI+SF | 25 806.0 | 225 | 9 100 | 18 730 | 7076.0 | |
LI+SF | 26 302.8 | 225 | 9 500 | 19 130 | 7172.8 |
表7 不同灌溉施肥模式的经济效益分析
Table 7 Economic benefit analysis of different irrigation and fertilization modes. 元/hm2
年份 Year | 处理 Treatment | 单位产值 Output value | 灌溉水成本 Irrigation water cost | 人工成本 Labor cost | 总成本 Total cost | 纯收入 Net income |
---|---|---|---|---|---|---|
2016 | RI+RF | 24 536.4 | 225 | 9 000 | 18 630 | 5906.4 |
LI+RF | 24 619.2 | 225 | 9 400 | 19 030 | 5589.2 | |
RI+SF | 24 867.6 | 225 | 9 100 | 18 730 | 6137.6 | |
LI+SF | 25 861.2 | 225 | 9 500 | 19 130 | 6731.2 | |
2017 | RI+RF | 25 005.6 | 225 | 9 000 | 18 630 | 6375.6 |
LI+RF | 25 171.2 | 225 | 9 400 | 19 030 | 6141.2 | |
RI+SF | 25 806.0 | 225 | 9 100 | 18 730 | 7076.0 | |
LI+SF | 26 302.8 | 225 | 9 500 | 19 130 | 7172.8 |
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