Chinese Journal of Rice Science

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Genetic Mode of bar Gene in Transgenic Rice

WU Ming-guo; HUA Zhi-hua; LIN Jian-rong; HUANG Da-nian   

  1. (China National Rice Research Institute; Hangzhou 310006; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-04-10 Published:2002-04-10

几个转bar基因水稻外源基因的遗传学行为研究

吴明国; 华志华; 林建荣; 黄大年   

  1. 中国水稻研究所, 浙江 杭州 310006

Abstract: The inheritance study of transgenic plants from four varieties and their stable lines demonstrated that bar gene was integrated into rice nucleus genome as a dominant gene. The herbicide resistance of transgenic stable lines TR3, TR4, TR5 and TR6 was controlled by a pair of dominant genes, and the bar gene of the four transgenic stable lines is non allelic one another. The bar genes at different loci have gene interaction, which behaving as gene overlapping action. Result of Southern analysis indicated that the bar gene of TR5 and TR6 is non allelic, and the bar gene at different loci could be recombined into the same rice nucleus genome by cross breeding.

Key words: gene transfer, rice, bar gene, herbicide resistance, inheritance

摘要: 利用春江03等4个品种的转基因株及后代稳定系进行遗传研究表明,bar基因作为一个显性单位已整合在水稻核基因组中,转基因株稳定系TR3、TR4、TR5、TR6对除草剂Basta的抗性均受1对显性基因控制,且4个转基因株稳定系间bar基因互不等位,不同位点的bar基因之间存在基因互作,表现为基因重叠作用。Southern杂交分析结果表明,TR5、TR6的bar基因整合位点互不相同,但可通过杂交育种重组到同一个水稻核基因组中。

关键词: 转基因, 水稻, bar基因, 除草剂抗性, 遗传