Mapping the Gene Wbph2 in ARC10239 Resistant to the Whitebacked Planthopper, Sogatella furcifera in Rice

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Mapping the Gene Wbph2 in ARC10239 Resistant to the Whitebacked Planthopper, Sogatella furcifera in Rice

LIU Zhi-yan ¹; LIU Guang-jie ^{1; *}; Kazushige SOGAWA ²; ZHUANG Jie-yun¹; CHEN Shen-guang ¹; SHEN Jun-hui ¹; ZHENG Kang-le ¹

（ ¹Chinese National Center for Rice Improvement; China National Rice Research Institute; Hangzhou 310006; China;²Japan International Research Center for Agricultural Sciences, Tsukuba 305-8686, Japan)

Received:1900-01-01 Revised:1900-01-01 Online:2002-10-10 Published:2002-10-10

水稻抗白背飞虱基因Wbph2的初步定位

刘志岩 1; 刘光杰1，*; 寒川一成2; 庄杰云1; 陈深广1; 沈君辉1; 郑康乐1

1中国水稻研究所国家水稻改良中心，浙江杭州 310006; 2日本国际农林水产业研究中心，日本筑波 3058686; *通讯联系人， E-mail:liug@mail.hz.zj.cn

Abstract

Abstract: The F₂ population of ARC10239/Minghui 63 was screened for the resistance to the whitebacked planthopper, Sogatella furcifera. The DNA extracted from the susceptible F₂ individuals was mixed, forming a susceptible DNA bulk. The polymorphisms in the resistant parent ARC10239, the susceptible parent Minghui 63 and the susceptible DNA bulk were tested by using 129 probes in combination with four enzymes of BamHⅠ, DraⅠ, EcoR Ⅴ and HindⅢ. Six positive probes were obtained, distributing in five rice chromosomes (Chromosome 3, 6, 8, 11 and 12). The positive probes were used to screen for the F₂ population of 142 individuals, revealing that Wbph2 was linked with the markers RZ667, RG64 and RG264 on the chromosome 6. Among the markers, the distance of RZ667 to Wbph2 was 25.6 cM (LOD=4.50).

Key words: Sogatella furcifera, rice, gene mapping, insect resistance, restricted fragment length polymophism

摘要： 鉴定了ARC10239/明恢63 F2群体对白背飞虱的抗性，混合F2感虫稻株的DNA，构建感虫池。应用129个RFLP探针，结合4种限制性内切酶，检测抗虫亲本ARC10239、感虫亲本明恢63和感虫DNA池的多态性。检测到6个阳性探针，分布在5个染色体上(染色体3、6、8、11和12)。应用阳性探针分析了F2群体的142个个体，发现抗虫基因Wbph2与第6染色体上的标记RZ667、RG64和RG264连锁,其中， Wbph2与RZ667的遗传距离为25.6 cM (LOD=4.50)。

关键词: 白背飞虱, 水稻, 基因定位, 抗虫性, 限制性片段长度多态性

LIU Zhi-yan ,LIU Guang-jie ,Kazushige SOGAWA ,ZHUANG Jie-yun ,CHEN Shen-guang ,SHEN Jun-hui ,ZHENG Kang-le . Mapping the Gene Wbph2 in ARC10239 Resistant to the Whitebacked Planthopper, Sogatella furcifera in Rice [J]. Chinese Journal of Rice Science.

刘志岩 , 刘光杰, 寒川一成, 庄杰云, 陈深广, 沈君辉, 郑康乐. 水稻抗白背飞虱基因Wbph2的初步定位[J]. 中国水稻科学.

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