Chinese Journal OF Rice Science ›› 2020, Vol. 34 ›› Issue (1): 1-7.DOI: 10.16819/j.1001-7216.2019.9101
• Research Papers • Next Articles
Xiaowu PAN, Yongchao LI, Wenqiang LIU, Haibo XIONG, Zheng DONG, Dan XUE, Xinnian SHENG, Wenjin ZHAO, Xiucai WEI, Xiaoxiang LI*()
Received:
2019-09-16
Revised:
2019-10-27
Online:
2020-01-10
Published:
2020-01-10
Contact:
Xiaoxiang LI
潘孝武, 黎用朝, 刘文强, 熊海波, 董铮, 薛丹, 盛新年, 赵文锦, 魏秀彩, 李小湘*()
通讯作者:
李小湘
基金资助:
CLC Number:
Xiaowu PAN, Yongchao LI, Wenqiang LIU, Haibo XIONG, Zheng DONG, Dan XUE, Xinnian SHENG, Wenjin ZHAO, Xiucai WEI, Xiaoxiang LI. Identification and Genetic Analysis of Waxy Sparse Mutant wax1 in Rice[J]. Chinese Journal OF Rice Science, 2020, 34(1): 1-7.
潘孝武, 黎用朝, 刘文强, 熊海波, 董铮, 薛丹, 盛新年, 赵文锦, 魏秀彩, 李小湘. 水稻蜡质稀少突变体wax1的鉴定及基因定位[J]. 中国水稻科学, 2020, 34(1): 1-7.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2019.9101
引物名称 Primer usage | 正向引物(5'→3') Forward primer (5'→3') | 反向引物(5'→3') Reverse primer (5'→3') |
---|---|---|
RM5806 | GAATGCTAATTGCGGTTGAAGC | GGATCTTTCCTCCCAATCTTTGC |
ID09139 | GAAACGGAGGGAGTATATCT | CTGTTTCCGATTCTAAGAGC |
RM25414 | GCTACCCAACCGCCTCCATAGC | GATCGATCGGAGAGCTTGAGTTGG |
ID09071 | AAGGTATTTCCCTCTCCTGA | AAGAAGAAGAAAAACCCAGG |
P1 | TCAACGAACACCAACAAGC | CAGATTATCGGCGGTCACT |
OsActin | CATTGGTGCTGAGCGTTTCC | AGAAACAAGCAGGAGGACGG |
OSH6 | GGTATTTGGTGGTGATTGTGTC | AACCCTTGAGTTGAGCCATT |
OsIAA1 | ACCAAGAGCCGCTCAATGAG | ATCACACGTGGGCGAACATC |
OsIAA4 | GCTCTTGCTGGATGGGTATGA | AGGTGATGGGCGTCTTGAAC |
OsIAA6 | GGCTATCGTCAGCTGTCAAACA | GCAATTTGCGCATTAGTTTGG |
OsIAA9 | CGAGAAGAAAATGGCCAATGA | ATCCCCATCACCATCCTCGTA |
OsIAA15 | CGTCCCCTGGAAAATGTTTG | TGCTAAATTGACTGCTTCAGAGCTT |
OsIAA18 | AAGAATGTGGGAAGGAGCTAACG | ATGGTGGTGAGGGACAGCAT |
Table 1 Primers for gene mapping and expression analysis.
引物名称 Primer usage | 正向引物(5'→3') Forward primer (5'→3') | 反向引物(5'→3') Reverse primer (5'→3') |
---|---|---|
RM5806 | GAATGCTAATTGCGGTTGAAGC | GGATCTTTCCTCCCAATCTTTGC |
ID09139 | GAAACGGAGGGAGTATATCT | CTGTTTCCGATTCTAAGAGC |
RM25414 | GCTACCCAACCGCCTCCATAGC | GATCGATCGGAGAGCTTGAGTTGG |
ID09071 | AAGGTATTTCCCTCTCCTGA | AAGAAGAAGAAAAACCCAGG |
P1 | TCAACGAACACCAACAAGC | CAGATTATCGGCGGTCACT |
OsActin | CATTGGTGCTGAGCGTTTCC | AGAAACAAGCAGGAGGACGG |
OSH6 | GGTATTTGGTGGTGATTGTGTC | AACCCTTGAGTTGAGCCATT |
OsIAA1 | ACCAAGAGCCGCTCAATGAG | ATCACACGTGGGCGAACATC |
OsIAA4 | GCTCTTGCTGGATGGGTATGA | AGGTGATGGGCGTCTTGAAC |
OsIAA6 | GGCTATCGTCAGCTGTCAAACA | GCAATTTGCGCATTAGTTTGG |
OsIAA9 | CGAGAAGAAAATGGCCAATGA | ATCCCCATCACCATCCTCGTA |
OsIAA15 | CGTCCCCTGGAAAATGTTTG | TGCTAAATTGACTGCTTCAGAGCTT |
OsIAA18 | AAGAATGTGGGAAGGAGCTAACG | ATGGTGGTGAGGGACAGCAT |
Fig. 1. Morphological traits of the wild type(WT) and its mutant waxy1. A and B, Plant morphology of the wild type and waxy1 mutant; C, Panicle morphology of the wild type and waxy1 mutant; D, Leaf morphology of the wild type and waxy1 mutant; E, Infiltration characteristics of leaf blade contaminated with water.
材料 Material | 株高 Plant height / cm | 有效穗数 No. of effective panicles | 每穗总粒数 No. of spikelets per panicle | 结实率 Seed-setting rate /% | 千粒重 1000-grain weight /g |
---|---|---|---|---|---|
WT | 81.4±3.4 | 5.5±0.5 | 134.3±6.7 | 81.8±6.3 | 23.9±0.4 |
wax1 | 61.2±2.3** | 7.4±0.2** | 94.6±4.0** | 77.7±2.5 | 21.9±0.3** |
Table 2 Agronomic traits of the wild type (WT) and wax1 mutant.
材料 Material | 株高 Plant height / cm | 有效穗数 No. of effective panicles | 每穗总粒数 No. of spikelets per panicle | 结实率 Seed-setting rate /% | 千粒重 1000-grain weight /g |
---|---|---|---|---|---|
WT | 81.4±3.4 | 5.5±0.5 | 134.3±6.7 | 81.8±6.3 | 23.9±0.4 |
wax1 | 61.2±2.3** | 7.4±0.2** | 94.6±4.0** | 77.7±2.5 | 21.9±0.3** |
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