Chinese Journal OF Rice Science ›› 2019, Vol. 33 ›› Issue (4): 323-330.DOI: 10.16819/j.1001-7216.2019.9027
• Research Papers • Previous Articles Next Articles
Xiaowu PAN, Wenqiang LIU, Yongchao LI, Haibo XIONG, Xinnian SHENG, Yonghong DUAN, Yaying YU, Wenjin ZHAO, Xiucai WEI, Xiaoxiang LI*()
Received:
2019-03-07
Revised:
2019-04-17
Online:
2019-07-10
Published:
2019-07-10
Contact:
Xiaoxiang LI
潘孝武, 刘文强, 黎用朝, 熊海波, 盛新年, 段永红, 余亚莹, 赵文锦, 魏秀彩, 李小湘*()
通讯作者:
李小湘
基金资助:
CLC Number:
Xiaowu PAN, Wenqiang LIU, Yongchao LI, Haibo XIONG, Xinnian SHENG, Yonghong DUAN, Yaying YU, Wenjin ZHAO, Xiucai WEI, Xiaoxiang LI. Identification and Genetic Analysis of Split Husk Mutant sh1 in Rice[J]. Chinese Journal OF Rice Science, 2019, 33(4): 323-330.
潘孝武, 刘文强, 黎用朝, 熊海波, 盛新年, 段永红, 余亚莹, 赵文锦, 魏秀彩, 李小湘. 水稻裂颖突变体sh1的鉴定及基因定位[J]. 中国水稻科学, 2019, 33(4): 323-330.
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2019.9027
引物名称Primer name | 正向引物 Forward primer (5'→3') | 反向引物 Reverse primer (5'→3') |
---|---|---|
RM15967 | AAGAGCCCTAGCGATAGCAAAGC | GTTCTACCTACCTGCGGCTTCC |
RM1352 | CACCTTCGATTTCCTTCTCATCG | CGAGTTGTACTCTGGTTGCTTCC |
RM1004 | GTCGACATGCTCGTGGTTCTGG | CCCAAGTTCGTCGACCTCACC |
ID19827 | ACCGTTCACTACAAGCAGAT | GTGTTCCACTTCCTCAACTG |
ID19884 | AAGTGGATCTCTCCGAAACT | TTCTGAGGGAGCTTATCGTA |
OsActin | CATTGGTGCTGAGCGTTTCC | AGAAACAAGCAGGAGGACGG |
OsJAmyb | GAGGACCAGAGTGCAAAAGC | CATGGCATCCTTGAACCTCT |
Table 1 Primers for gene mapping and expression analysis.
引物名称Primer name | 正向引物 Forward primer (5'→3') | 反向引物 Reverse primer (5'→3') |
---|---|---|
RM15967 | AAGAGCCCTAGCGATAGCAAAGC | GTTCTACCTACCTGCGGCTTCC |
RM1352 | CACCTTCGATTTCCTTCTCATCG | CGAGTTGTACTCTGGTTGCTTCC |
RM1004 | GTCGACATGCTCGTGGTTCTGG | CCCAAGTTCGTCGACCTCACC |
ID19827 | ACCGTTCACTACAAGCAGAT | GTGTTCCACTTCCTCAACTG |
ID19884 | AAGTGGATCTCTCCGAAACT | TTCTGAGGGAGCTTATCGTA |
OsActin | CATTGGTGCTGAGCGTTTCC | AGAAACAAGCAGGAGGACGG |
OsJAmyb | GAGGACCAGAGTGCAAAAGC | CATGGCATCCTTGAACCTCT |
材料 Material | 株高 Plant height / cm | 有效穗数 No. of effective panicles | 每穗总粒数 No. of spikelets per panicle | 结实率 Seed-setting rate / % | 千粒重 1000-grain weight / g |
---|---|---|---|---|---|
WT | 81.4±3.4 | 5.5±0.5 | 134.3±6.7 | 81.8±6.3 | 23.9±0.4 |
sh1 | 87.8±2.1 | 7.5±0.7* | 121.3±12.9 | 25.1±3.8** | 22.1±0.3* |
Table 2 Agronomic traits of the wild type (WT) and sh1 mutant.
材料 Material | 株高 Plant height / cm | 有效穗数 No. of effective panicles | 每穗总粒数 No. of spikelets per panicle | 结实率 Seed-setting rate / % | 千粒重 1000-grain weight / g |
---|---|---|---|---|---|
WT | 81.4±3.4 | 5.5±0.5 | 134.3±6.7 | 81.8±6.3 | 23.9±0.4 |
sh1 | 87.8±2.1 | 7.5±0.7* | 121.3±12.9 | 25.1±3.8** | 22.1±0.3* |
Fig. 4. Relative expression level of genes related to JA synthesis and signal transduction. *Significant difference between the mutant and WT at 0.05 level; **Significant difference between the mutant and WT at 0.01 level.
[1] | 陈柏原, 符辰建, 秦鹏, 张海清, 杨远柱. 水稻种子裂颖性的研究进展. 杂交水稻, 2014, 29(3): 6-9. |
Chen B Y, Fu C J, Qin P, Zhang H Q, Yang Y Z.Advances in studies on split husk of rice seeds.Hybrid Rice, 2014, 29(3): 6-9. (in Chinese with English abstract) | |
[2] | 杨建菊. 杂交水稻种子的裂颖及其危害. 杂交水稻, 2006, 21(1): 57-60. |
Yang J J.Split husk and its harm in hybrid rice seeds.Hybrid Rice, 2006, 21(1): 57-60. (in Chinese with English abstract). | |
[3] | 曾晓春, 周燮, 吴晓玉. 水稻颖花开放机理研究进展. 中国农业科学, 2004, 37(2): 188-195. |
Zeng X C, Zhou X, Wu X Y.Advances in study of opening mechanism in rice florets.Sci Agric Sin, 2004, 37(2): 188-195. (in Chinese with English abstract). | |
[4] | 管恩相, 李勇, 刘陵武, 姚仁祥, 郭君, 唐海燕. 三系不育系种子裂颖形成的原因及防治措施研究. 中国种业, 2010(1): 42-43. |
Guan E X, Li Y, Liu L W, Yao R X, Guo J, Tang H Y.Study on causes and control measures of split husk in three-line male sterile line seeds. China Seed Ind, 2010(1): 42-43. (in Chinese with English abstract). | |
[5] | 朱伟, 童继平, 吴跃进. 水稻品种抗裂颖资源的筛选与初步研究. 植物遗传资源学报, 2004, 5(1): 52-55. |
Zhu W, Tong J P, Wu Y J.Preliminary study and selection of rice germplasm with glume gaping resistance.J Plant Genet Resour, 2004, 5(1): 52-55. (in Chinese with English abstract). | |
[6] | 彭柏池, 刘晓霞, 张桂莲, 陈立云. 授粉期气象因子对水稻细胞质雄性不育系种子裂颖特性的影响. 中国农业气象, 2008, 29(3): 308-311. |
Peng B C, Liu X X, Zhang G L, Chen L Y.Influence of meteorological factors during pollination stage on characters of glume-gaping grains of cytoplasmic male sterile lines in rice.Chin J Agrometeorol, 2008, 29(3): 308-311. (in Chinese with English abstract). | |
[7] | 孙廉平, 张迎信, 张沛沛, 杨正福, 占小登, 沈希宏, 张振华, 胡霞, 轩丹丹, 吴玮勋, 曹立勇, 程式华. 一个由可变剪接造成的水稻开颖不育突变体ohms1的鉴定及基因定位. 中国水稻科学, 2015, 29(5): 457-466. |
Sun L P, Zhang Y X, Zhang P P, Yang Z F, Zhan X D, Shen X H, Zhang Z H, Hu X, Xuan D D, Wu W X, Cao L Y, Cheng S H.Characterization and gene mapping of an open hull male sterile mutant ohmsl caused by alternative splicing in rice.Chin J Rice Sci, 2015, 29(5): 457-466. (in Chinese with English abstract). | |
[8] | 陈立凯, 黄明, 刘永柱, 王慧, 陈志强, 郭涛. 水稻开颖半不育突变体的观察、遗传分析和基因定位. 中国农业科学, 2016, 49(1): 1-13. |
Chen L K, Huang M, Liu Y Z, Wang H, Chen Z Q, Guo T.Observation, genetic analysis and gene mapping of an open hull semi-sterility mutant in rice (Oryza sativa).Sci Agric Sin, 2016, 49(1): 1-13. (in Chinese with English abstract). | |
[9] | 曾生元, 郭旻, 李荣德, 盛生兰, 龚红兵, 严长杰. 水稻新裂颖突变体sg1的遗传分析与基因定位. 中国水稻科学, 2015, 29(1): 22-27. |
Zeng S Y, Guo M, Li R D, Sheng S L, Gong H B, Yan C J.Genetic analysis and fine mapping for a novel split glume mutant in rice.Chin J Rice Sci, 2015, 29(1): 22-27. (in Chinese with English abstract). | |
[10] | 沈亚林, 庄慧, 陈欢, 曾晓琴, 李香凝, 张君, 郑昊, 凌英华, 李云峰. 水稻颖花持续开放突变体sostenuto floret opening (sfo1)的鉴定与基因. 作物学报, 2017, 43(8): 1122-1127. |
Shen Y L, Zhuang H, Chen H, Zeng X Q, Li X N, Zhang J, Zheng H, Ling Y H, Li Y F.Characterization and gene mapping of sostenuto floret opening 1 (sfo1) mutant in rice(Oryza sativa L.).Act Agron Sin, 2017, 43(8): 1122-1127. (in Chinese with English abstract). | |
[11] | Li L, Shi C H, Zeng D D, Jin X L, Wu J G.Morphogenesis and molecular basis on the unclosed glumes, a novel mutation related to the floral organ of rice.Plant Mol Biol Rep, 2015, 33(3): 480-489. |
[12] | Li X, Wang Y, Duan E, Qi Q, Zhou K, Lin Q, Wang D, Wang Y, Long W, Zhao Z.OPEN GLUME1: A key enzyme reducing the precursor of JA, participates in carbohydrate transport of lodicules during anthesis in rice.Plant Cell Rep, 2018, 37(2): 329-346. |
[13] | Dhakarey R, Kodackattumannil P P, Riemann M.Functional analysis of jasmonates in rice through mutant approaches.Plants, 2016, 5(1): 1-15. |
[14] | Wasternack C, Song S.Jasmonates: Biosynthesis, metabolism, and signaling by proteins activating and repressing transcription.J Exp Bot, 2016, 68(6): 1303-1321. |
[15] | Cai Q, Yuan Z, Chen M, Yin C, Luo Z, Zhao X, Liang W, Hu J, Zhang D.Jasmonic acid regulates spikelet development in rice.Nat Commun, 2014, 5(3): 3476. |
[16] | 冯孟杰, 徐恒, 张华, 朱英. 茉莉素调控植物生长发育的研究进展. 植物生理学报, 2015, 51(4): 407-412. |
Feng M J, Xu H, Zhang H, Zhu Y.Recent progress in jasmonates regulation of plant growth and development.Plant Physiol J, 2015, 51(4): 407-412. (in Chinese with English abstract). | |
[17] | Wasternack C.Jasmonates: An update on biosynthesis, signal transduction and action in plant stress response, growth and development.Ann Bot, 2007, 100(4): 681-697. |
[18] | Ishiguro S, Kawai-Oda A, Ueda J, Nishida I, Okada K.The DEFECTIVE IN ANTHER DEHISCIENCE gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis.Plant Cell, 2001, 13(10): 2191-2209. |
[19] | Park J H, Halitschke R, Kim H B, Baldwin I T, Feldmann K A, Feyereisen R.A knock-out mutation in allene oxide synthase results in male sterility and defective wound signal transduction in Arabidopsis due to a block in jasmonic acid biosynthesis.Plant J, 2002, 31(1): 1-12. |
[20] | Zeng X C, Zhou X.Methyl jasmonate induces the opening of spikelets in rice.Chin Bull Bot, 1999, 41(5): 560-562. |
[21] | Song P, Xia K, Wu C W, Bao D P, Chen L P, Zhou X, Cao X Z.Differential response of floret opening in male-sterile and male-fertile rice to methyl jasmonate.Chin Bull Bot, 2001, 43(5): 480-485. |
[22] | Liu L, Zou Z, Qian K, Xia C, He Y, Zeng H, Zhou X, Riemann M, Yin C, Zhang D.Jasmonic acid deficiency leads to scattered floret opening time in cytoplasmic male sterile rice Zhenshan 97A. J Exp Bot, 2017, 68(16): 4613-4625. |
[23] | 黄俊宝, 何永明, 曾晓春, 向妙莲, 付永琦. 水稻颖花开放前花器官茉莉酸水平变化及浆片茉莉酸信号基因表达分析. 中国农业科学, 2015, 48(6): 1219-1227. |
Huang J B, He Y M, Zeng X C, Xiang M L, Fu Y Q.Changes of JA levels in floral organs and expression analysis of JA signaling genes in lodicules before floret opening in rice.Sci Agric Sin, 2015, 48(6): 1219-1227. (in Chinese with English abstract) | |
[24] | 付永琦, 向妙莲, 蒋海燕, 何永明, 曾晓春. 水稻颖花开放前浆片转录组变化. 中国农业科学, 2016, 49(6): 1017-1033. |
Fu Y Q, Xiang M L, Jiang H Y, He Y M, Zeng X C.Transcriptome profiling of lodicules before floret opening in Oryza sativa L.Sci Agric Sin, 2016, 49(6): 1017-1033. (in Chinese with English abstract). | |
[25] | Fu J, Chu J, Sun X, Wang J, Yan C.Simple, rapid, and simultaneous assay of multiple carboxyl containing phytohormones in wounded tomatoes by UPLC-MS/MS using single SPE purification and isotope dilution.Anal Sci, 2012, 28(11): 1081-1087. |
[26] | 韩同凯, 周晋军, 薛彦久, 陈翠霞, 谢先芝. 水稻丙二烯氧化物合成酶基因OsAOS1的表达研究. 山东农业科学, 2013, 45(5): 1-5. |
Han T K, Zhou J J, Xue Y J, Chen C X, Xie X Z.Expression analysis of allene oxide synthase gene, OsAOS1, in rice.Shandong Agric Sci, 2013, 45(5): 1-5. (in Chinese with English abstract) | |
[27] | Ken H, Moritoshi I.Phytochrome-mediated transcriptional up-regulation of ALLENE OXIDE SYNTHASE in rice seedlings.Plant Cell Physiol, 2004, 45(2): 119-128. |
[28] | Biswas K K, Ralf N, Ken H, Osamu Y, Moritoshi I.Photomorphogenesis of rice seedlings: A mutant impaired in phytochrome-mediated inhibition of coleoptile growth.Plant Cell Physiol, 2003, 44(3): 242-254. |
[29] | Hibara K I, Isono M, Mimura M, Sentoku N, Kojima M, Sakakibara H, Kitomi Y, Yoshikawa T, Itoh J I, Nagato Y.Jasmonate regulates juvenile-adult phase transition in rice.Development, 2016, 143(18): 3407-3416. |
[30] | Michael R, Ken H, Takafumi S, Kazunori O, Sugihiro A, Susumu M, Yoko N, Utako Y, Peter N, Masahiro Y.Identification of rice Allene Oxide Cyclase mutants and the function of jasmonate for defence against Magnaporthe oryzae.Plant J, 2013, 74(2): 226-238. |
[31] | Riemann M, Takano M.Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signaling.Plant, Cell & Environ, 2010, 31(6): 783-792. |
[32] | Xiao Y, Chen Y, Charnikhova T, Mulder P P J, Heijmans J, Hoogenboom A, Agalou A, Michel C, Morel J B, Dreni L. OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice. Plant Mol Biol, 2014, 86(1-2): 19-33. |
[33] | Chen Y, Ma J, Miller A J, Luo B, Wang M, Zhu Z, Ouwerkerk P B.OsCHX14 is involved in the K+ homeostasis in rice (Oryza sativa) flowers.Plant Cell Physiol, 2016, 57(7): 1530-1543. |
[1] | REN Zhiqi, XUE Kexin, DONG Zheng, LI Xiaoxiang, LI Yongzhao, GUO Yujing, LIU Wenqiang, GUO Liang, SHENG Xinnian, LIU Zhixi, PAN Xiaowu. Identification and Gene Mapping of Outcurved Leaf Mutant ocl1 in Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 337-346. |
[2] | XIAO Lequan, LI Lei, DAI Weimin, QIANG Sheng, SONG Xiaoling. Seedling Growth Characteristics of Hybrids Between Transgenic Rice with cry2A*/bar Genes and Weedy Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 347-358. |
[3] | LI Gang, GAO Qingsong, LI Wei, ZHANG Wenxia, WANG Jian, CHEN Baoshan, WANG Di, GAO Hao, XU Weijun, CHEN Hongqi, JI Jianhui. Directed Knockout of SD1 Gene Improves Lodging Resistance and Blast Resistance of Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 359-367. |
[4] | WANG Shengyong, CHEN Yuhang, CHEN Huili, HUANG Yujie, ZHANG Xiaotian, DING Shuangcheng, WANG Hongwei. Effects of High Temperature on Phenylpropane Metabolism and Downstream Branch Metabolic Pathways in Rice Meiosis [J]. Chinese Journal OF Rice Science, 2023, 37(4): 368-378. |
[5] | HUANG Yaru, XU Peng, WANG Lele, HE Yizhe, WANG Hui, KE Jian, HE Haibing, WU Liquan, YOU Cuicui. Effects of Exogenous Trehalose on Grain Filling Characteristics and Yield Formation of japonica Rice Cultivar W1844 [J]. Chinese Journal OF Rice Science, 2023, 37(4): 379-391. |
[6] | DONG Liqiang, YANG Tiexin, LI Rui, SHANG Wenqi, MA Liang, LI Yuedong, SUI Guomin. Effect of Plant-row Spacing on Rice Yield and Root Morphological and Physiological Characteristics in Super High Yield Field [J]. Chinese Journal OF Rice Science, 2023, 37(4): 392-404. |
[7] | GAO Qianqing, REN Xiaojian, ZHAI Zhongbing, ZHENG Pubing, WU Yuanfen, CUI Kehui. Effects of Panicle and Bud-promoting Nitrogen Fertilizer Application on Growth of Regenerated Bud and Grain Yield of Ratoon Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 405-414. |
[8] | HUANG Jinwen, LI Rikun, CHEN Zhicheng, ZHANG Bianhong, LEI Han, PAN Ruixin, YANG Mingyu, PAN Meiqing, TANG Lina. Effects of Straw Returning Techniques on Soil Nutrients, Organic Carbon and Microbial Diversity in Tobacco-rice Rotation System [J]. Chinese Journal OF Rice Science, 2023, 37(4): 415-426. |
[9] | HAN Cong, HE Yuchang, WU Lijuan, JIA Lili, WANG Lei, E Zhiguo. Research Progress in the Function of Basic Leucine Zipper (bZIP) Protein Family in Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 436-448. |
[10] | SHEN Yumin, CHEN Mingliang, XIONG Huanjin, XIONG Wentao, WU Xiaoyan, XIAO Yeqing. Phenotypic Analysis and Fine Mapping of blg1(beak like grain 1), a Rice Mutant with Abnormal Palea and Lemma Development [J]. Chinese Journal OF Rice Science, 2023, 37(3): 225-232. |
[11] | DUAN Min, XIE Liujie, GAO Xiuying, TANG Haijuan, HUANG Shanjun, PAN Xiaobiao. Creation of Thermo-sensitive Genic Male Sterile Rice Lines with Wide Compatibility Based on CRISPR/Cas9 Technology [J]. Chinese Journal OF Rice Science, 2023, 37(3): 233-243. |
[12] | WANG Wenting, MA Jiaying, LI Guangyan, FU Weimeng, LI Hubo, LIN Jie, CHEN Tingting, FENG Baohua, TAO Longxing, FU Guanfu, QIN Yebo. Effect of Different Fertilizer Application Rates on Rice Yield and Quality Formation and Its Relationship with Energy Metabolism at High Temperature [J]. Chinese Journal OF Rice Science, 2023, 37(3): 253-264. |
[13] | LIU Aihua, LI Xiaokun. Meta-analysis of Relationship Between Fertilizer Application and Rice Quality [J]. Chinese Journal OF Rice Science, 2023, 37(3): 276-284. |
[14] | YANG Xiaolong, WANG Biao, WANG Benfu, ZHANG Zhisheng, ZHANG Zuolin, YANG Lantian, CHENG Jianping, LI Yang. Effects of Different Water Management on Yield and Rice Quality of Dry-seeded Rice [J]. Chinese Journal OF Rice Science, 2023, 37(3): 285-294. |
[15] | WEI Xiaodong, SONG Xuemei, ZHAO Ling, ZHAO Qingyong, CHEN Tao, LU Kai, ZHU Zhen, HUANG Shengdong, WANG Cailin, ZHANG Yadong. Effects of Silicon and Zinc Fertilizer and Their Application Ways on Yield and Grain Quality of Rice Variety Nanjing 46 [J]. Chinese Journal OF Rice Science, 2023, 37(3): 295-306. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||