Physiological Changes in Rice Plants After Infestation by Whitebacked Planthoppers at Different Nitrogen Levels

doi:10.16819/j.1001-7216.2018.8012

Abstract

Abstract:

【Objective】In order to clarify the physiological mechanism in regulating the resistance of rice to whitebacked planthoppers at different nitrogen levels,【Method】we compared the differences in contents of chlorophyll, relative water content, bleeding sap, water content, dry matter content, soluble sugar contents in infected rice at different nitrogen levels by indoors cage-raising method.【Result】The results showed that there were significant changes in different defensive substances in rice after infection by whitebacked planthoppers. The contents of chlorophyll, dry matter weight and soluble sugar contents in rice plants decreased after BPH infection, while the content of bleeding sap, relative water content and water content increased. With increasing nitrogen levels, chlorophyll (SPAD) contents and dry matter weight of rice increased, and the increase of water content and bleeding sap content followed an upward trend. Water content, soluble sugar contents were on a decreasing trend. 【Conclusion】Nitrogen level could affect the resistance of rice to whitebacked planthoppers by regulating the contents of chlorophyll (SPAD), relative water content, water content, dry matter weight and soluble sugar contents in rice, subsequently influencing the population of whitebacked planthoppers.

Key words: rice, nitrogen level, whitebacked planthoppers, defensive substance

摘要：

【目的】为明确不同施氮水平调控水稻对白背飞虱抵御能力的生理机制,【方法】采用室内笼罩饲喂胁迫法,比较了不同施氮水平下水稻受白背飞虱为害后,体内叶绿素(SPAD)含量、含水量、伤流液含量和干物质含量、可溶性糖含量等防御相关物质含量的变化。【结果】水稻受白背飞虱为害后,水稻体内不同防御物质含量变化存在差异。水稻受白背飞虱为害后体内叶绿素含量、干物质含量、可溶性糖含量均有所下降,伤流液含量、相对含水量、含水量有所上升。随着水稻施氮水平的增加,受害水稻叶绿素含量、干物质量降幅呈上升趋势,含水量、伤流液含量增幅呈上升趋势,相对含水量、可溶性糖含量增幅呈下降趋势。【结论】不同施氮水平可通过调控水稻体内叶绿素含量、相对含水量、含水量、干物质含量、可溶性糖含量影响水稻对白背飞虱的抵御能力,从而影响白背飞虱种群数量。

关键词: 水稻, 施氮水平, 白背飞虱, 抵御物质

CLC Number:

Zaiyuan LI, Bo XU, Fulian WANG, Xiaohai TIAN, Yuekun MA, Yujun ZHONG, Shiyang CHENG. Physiological Changes in Rice Plants After Infestation by Whitebacked Planthoppers at Different Nitrogen Levels[J]. Chinese Journal OF Rice Science, 2018, 32(5): 501-508.

李再园, 许博, 王福莲, 田小海, 马跃坤, 钟裕俊, 程世阳. 不同施氮水平下水稻植株在白背飞虱为害后的生理变化[J]. 中国水稻科学, 2018, 32(5): 501-508.

Figures/Tables 6

Fig. 1. Changes in chlorophyll contents in rice at various nitrogen levels as infected by whitebacked planthoppers. Various lowercase letters above or under the bars mean that the fluctuation and increment of leaf chlorophyll contents in rice at a given nitrogen level after WBPH infection are significantly different at 0.05 level.

Fig. 2. Changes in water content in rice at various nitrogen levels as infected by whitebacked planthoppers.

Fig. 3. Changes in bleeding sap content in rice at various nitrogen levels as affected by whitebacked planthoppers.

Fig. 4. Changes in dry matter weight in rice at various nitrogen levels as affected by whitebacked planthoppers.

Fig. 5. Changes in soluble sugar contents in rice at various nitrogen levels as infected by whitebacked planthoppers.

Table 1 Correlative coefficients and significance of physiological substances and nitrogen level in rice infested with whitebacked planthoppers.

参数 Parameters	叶绿素含量 Chlorophyll content	相对含水量 Relative water content	含水量 Water content	伤流液量 Bleeding sap content	干物质含量 Dry matter weight	可溶性糖含量 Soluble sugar content
R值R value	0.685	–0.276	0.471	0.059	–0.402	–0.394
P值P value	0.133	0.597	0.345	0.917	0.430	0.440

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