Chinese Journal OF Rice Science ›› 2017, Vol. 31 ›› Issue (6): 580-589.DOI: 10.16819/j.1001-7216.2017.6152
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Yuqiong WANG1, Yaolong YANG1, Yujia LENG1, Lichao HUANG1, Long CHEN1, Liping DAI1, Zhengjun TU1, Yihong GAO1, Jiang HU1, Li ZHU1, Guangheng ZHANG1, Deyong REN1, Zhenyu GAO1, Guojun DONG1, Guang CHEN1, Longbiao GUO1, Guoyou YE2, Qian QIAN1,*(), Dali ZENG1,*()
Received:
2017-11-05
Revised:
2017-04-03
Online:
2017-11-25
Published:
2017-11-10
Contact:
Qian QIAN, Dali ZENG
汪玉琼1, 杨窑龙1, 冷语佳1, 黄李超1, 陈龙1, 代丽萍1, 涂政军1, 高易宏1, 胡江1, 朱丽1, 张光恒1, 任德勇1, 高振宇1, 董国军1, 陈光1, 郭龙彪1, 叶国友2, 钱前1,*(), 曾大力1,*()
通讯作者:
钱前,曾大力
基金资助:
CLC Number:
Yuqiong WANG, Yaolong YANG, Yujia LENG, Lichao HUANG, Long CHEN, Liping DAI, Zhengjun TU, Yihong GAO, Jiang HU, Li ZHU, Guangheng ZHANG, Deyong REN, Zhenyu GAO, Guojun DONG, Guang CHEN, Longbiao GUO, Guoyou YE, Qian QIAN, Dali ZENG. Identification and Fine Mapping of Small Grain Gene SG101 in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2017, 31(6): 580-589.
汪玉琼, 杨窑龙, 冷语佳, 黄李超, 陈龙, 代丽萍, 涂政军, 高易宏, 胡江, 朱丽, 张光恒, 任德勇, 高振宇, 董国军, 陈光, 郭龙彪, 叶国友, 钱前, 曾大力. 水稻小粒基因SG101的鉴定和精细定位[J]. 中国水稻科学, 2017, 31(6): 580-589.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2017.6152
引物 Marker | 前引物序列 Forward primer (5′-3′) | 后引物序列 Reverse primer (5′-3′) |
---|---|---|
UBQ5 | CTCGCCGACTACAACATCCA | TCTTGGGCTTCCTCTACGTCTT |
E2F2 | TGTTGGTGGCTGCCGATAT | CGCCAGGTGCACCCTTT |
CYCT1;2 | GCATTTGTTGCAGCTCAAG | TCACCACTTCGCTGACTTATTG |
CYCD4;1 | GCCATGGAGTTGATACATCCAA | CCAGTAGGGCTCCGTGGAAT |
CYCD7;1 | CCTTCCACACTGACGGTACAGTT | TGCCGCTGCCAAATAGACA |
CYCB2;2 | CTCAAGGCTGCACAATCTGACA | GCATTGACGGCTGGAATTTG |
D2 | TTCAACCCATGGAGGTGGAA | GCACGGTGGGGAAGTTGACGA |
OsCPOD | TTCTTCTCCATCCCCTTTCCTCTCGCCA | CACCCTCCGCCTCAAGAAGCTCCTCAA |
DWARF4Q | GAGATGGTTTTCACGCAATGTG | ACCCTTGTAGTGCACGTCCTTG |
BU1 | GTAGCCAGCTTGATCTCATCTC | GGGACGACTCTACTGCATCA |
BZRF | CTCGGCAGCGTCGAGGTGC | AGGAATTGTTGCTGAGCTTC |
D61 | CTCGGCAGCGTCGAGGTGC-3' | AGGAATTGTTGCTGAGCTTC |
PAVL1 | GCACTCCTCGTTGGATCTCGAT | GCAGCAGACGGAAGATGGATT |
OsLIC | GGAGTTTCGAGCGTATCTGGAA | TGGACAGAGGAAGCAGGAGACT |
OsMDP1 | TTATTGACCGGTACAACTCGCA | TCCAGTCCATCGATCTCATCC |
Table 1 Primers used for real-time RT-PCR in the study.
引物 Marker | 前引物序列 Forward primer (5′-3′) | 后引物序列 Reverse primer (5′-3′) |
---|---|---|
UBQ5 | CTCGCCGACTACAACATCCA | TCTTGGGCTTCCTCTACGTCTT |
E2F2 | TGTTGGTGGCTGCCGATAT | CGCCAGGTGCACCCTTT |
CYCT1;2 | GCATTTGTTGCAGCTCAAG | TCACCACTTCGCTGACTTATTG |
CYCD4;1 | GCCATGGAGTTGATACATCCAA | CCAGTAGGGCTCCGTGGAAT |
CYCD7;1 | CCTTCCACACTGACGGTACAGTT | TGCCGCTGCCAAATAGACA |
CYCB2;2 | CTCAAGGCTGCACAATCTGACA | GCATTGACGGCTGGAATTTG |
D2 | TTCAACCCATGGAGGTGGAA | GCACGGTGGGGAAGTTGACGA |
OsCPOD | TTCTTCTCCATCCCCTTTCCTCTCGCCA | CACCCTCCGCCTCAAGAAGCTCCTCAA |
DWARF4Q | GAGATGGTTTTCACGCAATGTG | ACCCTTGTAGTGCACGTCCTTG |
BU1 | GTAGCCAGCTTGATCTCATCTC | GGGACGACTCTACTGCATCA |
BZRF | CTCGGCAGCGTCGAGGTGC | AGGAATTGTTGCTGAGCTTC |
D61 | CTCGGCAGCGTCGAGGTGC-3' | AGGAATTGTTGCTGAGCTTC |
PAVL1 | GCACTCCTCGTTGGATCTCGAT | GCAGCAGACGGAAGATGGATT |
OsLIC | GGAGTTTCGAGCGTATCTGGAA | TGGACAGAGGAAGCAGGAGACT |
OsMDP1 | TTATTGACCGGTACAACTCGCA | TCCAGTCCATCGATCTCATCC |
Fig. 1. Phenotypes of Zhonghua 11(WT) and sg101. **Significant difference at 0.01 level between wild type and mutant sg101. The same as that in Fig. 2 and Fig. 3.
性状 Trait | 野生型 Wild type | 突变体 sg101 |
---|---|---|
株高Plant height/cm | 88.8±2.4 | 67.6±6.1 |
穗长Panicle length/cm | 22.7±1.1 | 17.1±1.2 |
着粒密度Spikelet density | 11.8±1.3 | 9.9±1.4 |
一次枝梗数Primary rachis branch number per panicle | 15.0±1.0 | 13.0±3.0 |
二次枝梗数Secondary rachis branch number per panicle | 51.3±11.1 | 33.6±7.1 |
每穗颖花数Spikelet number per panicle | 270.0±40.0 | 170.0±29.3 |
结实率Seed setting rate/% | 70.0±4.3 | 5.0±3.2 |
Table 2 Comparison of panicle-related traits between Zhonghua 11 and sg101.
性状 Trait | 野生型 Wild type | 突变体 sg101 |
---|---|---|
株高Plant height/cm | 88.8±2.4 | 67.6±6.1 |
穗长Panicle length/cm | 22.7±1.1 | 17.1±1.2 |
着粒密度Spikelet density | 11.8±1.3 | 9.9±1.4 |
一次枝梗数Primary rachis branch number per panicle | 15.0±1.0 | 13.0±3.0 |
二次枝梗数Secondary rachis branch number per panicle | 51.3±11.1 | 33.6±7.1 |
每穗颖花数Spikelet number per panicle | 270.0±40.0 | 170.0±29.3 |
结实率Seed setting rate/% | 70.0±4.3 | 5.0±3.2 |
Fig. 2. The comparison of grain between Zhonghua 11(WT) and sg101. A, Developed lemma; B and C, Paraffin section of the lemmas of wild-type and sg101, respectively. D, Cell number of the lemma; E and F, SEM analysis of the lemma outer surface of wild-type and sg101, respectively. G, Cell length of the outer epidermal lemma.
Fig. 4. Lamina joint test to 2,4-epiBL in Zhonghua 11(WT) and sg101. A, Performance of lamina joint to 2,4-epiBL in wild-type(up) and sg101(down). Scale bars, 2.0 cm; B and C, Changes of lamina inclination and coleoptile length to 2,4-epiBL, respectively. *, **Significant difference at 0.05 and 0.01 level between wild type and mutant sg101, respectively.
分离群体 Segregation population | F1 | F2 | χ2 (3:1) | P值 P-value | |||
---|---|---|---|---|---|---|---|
正常表型 Wild-type phenotype | 突变表型 sg101 phenotype | 正常表型 Wild-type phenotype | 突变表型 sg101 phenotype | ||||
sg101/TN1 | 4 | 0 | 116 | 34 | 0.436 | 0.509 | |
sg101/南京6号 sg101/Nanjing 6号 | 8 | 0 | 258 | 74 | 1.301 | 0.254 | |
sg101/9311 | 5 | 0 | 154 | 43 | 1.058 | 0.304 | |
TN1/sg101 | 4 | 0 | 128 | 38 | 0.394 | 0.530 | |
南京6号/sg101 Nanjing 6/sg101 | 3 | 0 | 102 | 29 | 0.573 | 0.449 | |
9311/sg101 | 6 | 0 | 178 | 56 | 0.143 | 0.076 |
Table 3 Genetic analysis of SG101.
分离群体 Segregation population | F1 | F2 | χ2 (3:1) | P值 P-value | |||
---|---|---|---|---|---|---|---|
正常表型 Wild-type phenotype | 突变表型 sg101 phenotype | 正常表型 Wild-type phenotype | 突变表型 sg101 phenotype | ||||
sg101/TN1 | 4 | 0 | 116 | 34 | 0.436 | 0.509 | |
sg101/南京6号 sg101/Nanjing 6号 | 8 | 0 | 258 | 74 | 1.301 | 0.254 | |
sg101/9311 | 5 | 0 | 154 | 43 | 1.058 | 0.304 | |
TN1/sg101 | 4 | 0 | 128 | 38 | 0.394 | 0.530 | |
南京6号/sg101 Nanjing 6/sg101 | 3 | 0 | 102 | 29 | 0.573 | 0.449 | |
9311/sg101 | 6 | 0 | 178 | 56 | 0.143 | 0.076 |
引物 Marker | 前引物序列 Forward primer(5′-3′) | 后引物序列 Reverse primer(5′-3′) | |
---|---|---|---|
M1 | GCTAGGCTTGATCCGAGAGA | GTCCCGCTTCTTCCCCTA | |
M2 | GACCACGTCAAGCATAAGTTCA | AGCCTTTCAGGGAGGAAGAA | |
M3 | CTCCCTACTTCTCCTCTTCG | CATACGCAACACCGCATCTT | |
M4 | GGTGTTTGATCCCATTTGCT | TGGATGAAGAACTGCGCATA | |
S1 | GGAACGGGGTGACAATTCTA | GCCTGTTTGAGGGAATGGTA | |
S2 | ATTCTTACTTAATAATCAATAC | TACCTAGCTCGACTCAGACCCA | |
S3 | GAGCAGGCAGACCTGCATAA | AAAACACTGCCTGTGATTCTGT | |
S4 | CGACGGTGGGAGCAGAAGAAA | TCGGACTATCTCTGCTGGAATT | |
S5 | ACTCCTATGTTTCAACCTG | GTGACCATCAATGAACCTT | |
S6 | AAATCTAGTCAACACGCCGT | ACATGAGTTTAATTCCAAAATT |
Table 4 Primers used for fine mapping of SG101 in this study.
引物 Marker | 前引物序列 Forward primer(5′-3′) | 后引物序列 Reverse primer(5′-3′) | |
---|---|---|---|
M1 | GCTAGGCTTGATCCGAGAGA | GTCCCGCTTCTTCCCCTA | |
M2 | GACCACGTCAAGCATAAGTTCA | AGCCTTTCAGGGAGGAAGAA | |
M3 | CTCCCTACTTCTCCTCTTCG | CATACGCAACACCGCATCTT | |
M4 | GGTGTTTGATCCCATTTGCT | TGGATGAAGAACTGCGCATA | |
S1 | GGAACGGGGTGACAATTCTA | GCCTGTTTGAGGGAATGGTA | |
S2 | ATTCTTACTTAATAATCAATAC | TACCTAGCTCGACTCAGACCCA | |
S3 | GAGCAGGCAGACCTGCATAA | AAAACACTGCCTGTGATTCTGT | |
S4 | CGACGGTGGGAGCAGAAGAAA | TCGGACTATCTCTGCTGGAATT | |
S5 | ACTCCTATGTTTCAACCTG | GTGACCATCAATGAACCTT | |
S6 | AAATCTAGTCAACACGCCGT | ACATGAGTTTAATTCCAAAATT |
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