Identification and Genetic Mapping of a Lesion Mimic Stripe Mutant in Rice
MA Jian-yang1, 2, CHEN Sun-lu2, 3, ZHANG Jian-hui1, 2, DONG Yan-jun 1,*, TENG Sheng2,*
(1Laboratory of Plant Genetics and Functional Gene, College of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China; 2Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 3National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; *Corresponding author, E-mail: email@example.com; firstname.lastname@example.org)
Abstract A lesion mimic stripe mutant was obtained from the M2 progeny of a japonica variety Jiahua 1 by 60Co γ radiation,temporarily designated as lms1 (lesion mimic stripe 1). The mutant displayed lesion mimic in its whole life, and belonged to propagation type. Physiological and histochemistry analysis suggested that the mutant displayed a phenotype of white stripe at a high temperature (30℃) and lesion mimic caused by cell initiative death at a low temperature(20℃)，respectively. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene. In addition, using SSR markers and an F2 segregating population derived from two crosses(lms1/9311 and Pei’ai 64S/lms1), the lms1 gene was mapped between Indel1 and MM0112-4 with a physical distance of 400 kb on chromosome 6 in rice.
MA Jian-yang,CHEN Sun-lu,ZHANG Jian-hui et al. Identification and Genetic Mapping of a Lesion Mimic Stripe Mutant in Rice[J]. , 2011, 25(2): 150-156 .
王忠华．植物类病变突变体的诱发与突变机制．细胞生物学杂志, 2005, 27(5)： 530-534. 王建军, 朱旭东, 王友林, 等．水稻类病斑突变体的生理与遗传分析．植物生理与分子生物学报, 2004, 30（3）: 331-338. Dietrich R A, Delanev T P, Uknes S J, et al. Arabidopsis mutants simulating disease resistance response. Cell, 1994, 77: 565-577. Buschges R, Hollricher K, Panstruga R, et al. The barley mlo gene: A novel control element of plant pathogen resistance. Cell, 1997, 88: 695-705. Gray J, Close P S, Briggs S P, et al. A novel suppressor of cell death in plants encoded by the Lls1 gene of maize. Cell, 1997, 89: 25-31． Badigannavar A M, Kale D M, Eapen S, et al. Inheritance of disease lesion mimic leaf trait in groundnut. J Hered, 2002, 93: 50-52. Malamy J, Carr J P, Klessig D F. Salicylic acid: A likely endogenous signal in the resistance response of tobacco to viral infection. Science, 1990, 250: 1002-1004. Takahashi A, Kawasaki T, Henmi K, et al. Lesion mimic mutants of rice with alterations in early signaling events of defense. Plant J, 1999, 17: 535-545. Dangl J L, Dietrich R A, Richberg M H. Death don′t have no mercy: Cell death programs in plant-microbe interactions. Plant Cell, 1996, 8: 1793-1807. Neuffer M G, Calver O H. Dominant disease lesion mimics in maize. J Hered, 1975, 66: 265-270. Shirasu K, Schulze-Lefert P. Regulators of cell death in disease resistance. Plant Mol Biol, 2000, 44: 371-385. Dietrich R A, Richberg M H, Schmidt R, et al. A novel zinc finger protein is encoded by the Arabidopsis lsd1 gene and functions as a negative regulator of plant cell death. Cell, 1997, 88: 685-694. Ryerson D E, Heath M C. Cleavage of nuclear DNA oligonucleosomal fragments during cell death induced by fungal infection or by abiotic treatments. Plant Cell, 1996, 8: 393-402. Lamb C, Dixon R A. The oxidative burst in plant disease resistance. Annu Rev Plant Physiol Plant Mol Biol, 1997, 48: 251-275. Asai T, Stone J M, Heard J E, et al. Fumonisin B1-induced cell death in Arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways. Plant Cell, 2000, 12: 1823-1836. Beffa R, Szell M, Meuwly P, et al. Cholera toxin elevates pathogen resistance and induces pathogenesis-related gene expression tobacco. EMBO J, 1995, 14: 5753-5761. Mittler R, Shulaev V, Lam E. Coordinated activation of programmed cell death and defense mechanisms in transgenic tobacco plants expressing a bacterial proton pump. Plant Cell, 1995, 7: 29-42. Yamanouchi U, Yano M, Lin H, et al. A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein. Proc Natl Acad Sci USA, 2002, 99: 7530-7535. 姜丽, 刘国庆, 韩建民, 等. 植物类病变坏死突变体及其基因的研究进展. 中国生物工程杂志, 2003, 23(1): 34-38. 王忠华, 贾育林, 夏英武, 等. 植物抗病分子机制研究进展. 植物学通报, 2004, 21(5): 521-530. Zeng L R, Qu S H, Bordeos A, et al. Spotted leaf11, a negative regulator of plant cell death and defense, encodes a U-box/armadillo repeat protein endowed with E3 ubiquitin ligase activity. Plant Cell, 2004, 16: 2795-2808. Collins N, Drake J, Ayliffe M, et al. Molecular characterization of the maize Rp1-D rust resistance haplotype and its mutants. Plant Cell, 1999, 11(7): 1365-1376. Wang L, Pei Z, Tian Y, et al. OsLSD1, a rice zinc finger protein, regulates programmed cell death and callus differentiation. Mol Plant Microbe Interact, 2005, 18: 375-348. Qiao Y L, Jiang W Z, Lee J H, et al. Spl28 encodes a clathrin-associated adaptor protein complex 1, medium subunit l1 (AP1M1) and is responsible for spotted leaf and early senescence in rice(Oryza sativa). New Phytol, 2010, 185: 258-274. Wu C J, Bordeos A, Madamba M A, et al. Rice lesion mimic mutants with enhanced resistance to diseases. Mol Genet Genomics, 2008, 279: 605-619. Matin M N, Pandeya D, Baek K H, et al. Phenotypic and genotypic analysis of rice lesion mimic mutants. Plant Pathol J, 2010, 26(2): 159-169. 高俊凤. 植物生理学实验指导. 北京: 高等教育出版社, 2006: 74-76. Yin Z, Chen J, Zeng L, et al. Characterizing rice lesion mimic mutants and identifying a mutant with broad-spectrum resistance to rice blast and bacterial blight. Mol Plant-Microbe Interact, 2000, 13: 869-876. Thordal-Christansen H, Zhang Z G, Wei Y D, et al. Subcellular localization of H2O2 in plants:H2O2 accumulation in papillae and hypersensitive response during the barley powdery mildew interaction. Plant J, 1997, 11(6): 1187-1194. Muray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8(19): 4321-4325. Lincoln S, Daly M, Lander E. Constructing genetics maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report. 3rd ed. Cambridge, Massachusetts: Whitehead Institute, 1992. 沈利爽, 郑先武, 朱立煌. Mapplotter——一个输出遗传图谱、图示基因型和QTL曲线图形的软件. 遗传, 2000, 22(3): 172-174. 黄奇娜, 杨杨, 施勇烽, 等. 水稻斑点叶变异研究进展. 中国水稻科学, 2010, 24(2): 108-115. Hoisington D A, Neuffer M G, Walbot V. Disease lesion mimics in maize: Ⅰ. Effect of genetic background, temperature, developmental age, and wounding on necrotic spot formation with Les1. Dev Biol, 1982, 93: 381-388. Hu G, Richter T E, Hulbert S H, et al. Disease lesion mimicry caused by mutations in the rust resistance gene rp1. Plant Cell, 1996, 8: 1367-1376. Johal G S, Hulbert S, Briggs S P. Disease lesion mimic mutations of maize: A model for cell death in plants. BioEssays, 1995, 17: 685-692. Jodon N E.Inheritance of some of the more striking character in rice. J Hered, 1957, 48(4): 181-192. Nagao S, Takahashi M, Morimura K. Causal genes and their linkage relationships of some morphological characters introduced from foreign rice varieties: Genetical studies on rice plant, ⅩⅩⅧ. Mem Fac Agr Hokkaido Univ, 1964, 5(2): 89-96. Nagao S, Takahashi M, Kinoshita T.New members of “wx”and “d1” linkage groups in rice. Jpn J Breed, 1966, 16: 60. Mizobuchi K, Hirabayashi H, Kaji R, et al. Developmental responses of resistance to Magnaporthe grisea and Xanthomonas campestris pv. oryzae in lesion-mimic mutants of rice. Breeding Sci, 2003, 53: 93-100. Mizobuchi R, Hirabayashi H, Kaji R, et al. Isolation and characterization of rice lesion-mimic mutants with enhanced resistance to rice blast and bacterial blight. Plant Sci, 2002, 163: 345-353.