Identification and Genetic Mapping of a Lesion Mimic Stripe Mutant in Rice

doi:10.3969/j.issn.1001-7216.2011.02.005

Abstract

Abstract: A lesion mimic stripe mutant was obtained from the M2 progeny of a japonica variety Jiahua 1 by 60Co γ radiation,temporarily designated as lms1 (lesion mimic stripe 1). The mutant displayed lesion mimic in its whole life, and belonged to propagation type. Physiological and histochemistry analysis suggested that the mutant displayed a phenotype of white stripe at a high temperature (30℃) and lesion mimic caused by cell initiative death at a low temperature(20℃)，respectively. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene. In addition, using SSR markers and an F2 segregating population derived from two crosses(lms1/9311 and Pei’ai 64S/lms1), the lms1 gene was mapped between Indel1 and MM0112-4 with a physical distance of 400 kb on chromosome 6 in rice.

Key words: rice, lesion mimic mutant, gene mapping

摘要： 粳稻品种嘉花1号经60Co γ射线辐射诱变后获得一个水稻类病斑突变体MR07, 暂命名为lms1。该突变体的病斑在全生育期均表现，属于扩散型类病斑突变体。生理和组织化学分析表明，该突变体在高温条件下（30℃）培养时只表现为白色条斑，低温条件下（20℃）培养时表现出细胞自主性死亡的坏死病斑。遗传分析表明，该突变体受1对隐性核基因控制。以lms1突变体与籼稻9311、培矮64S杂交的两个F2分离群体作为定位群体，利用SSR标记和Indel标记将该基因定位在第6染色体上，位于标记InDel1和MM0112-4之间，其物理距离为400 kb。

关键词: 水稻, 类病斑突变体, 基因定位

MA Jian-yang,CHEN Sun-lu,ZHANG Jian-hui,DONG Yan-jun ,TENG Sheng. Identification and Genetic Mapping of a Lesion Mimic Stripe Mutant in Rice[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2011.02.005 .

马健阳, 陈孙禄, , 张建辉, , 董彦君, 滕胜, . 一个水稻类病条纹斑突变体的鉴定和遗传定位[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2011.02.005 .

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