中国水稻科学
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  2011, Vol. 25 Issue (2): 136-142     DOI: 10.3969/j.issn.1001-7216.2011.02.003
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Prokaryotic Expression of Polygalacturonase-Inhibiting Protein Gene (Ospgip1) from Rice and Bioinformatics Analysis of Its Coding Product

CHEN Xi-jun 1, 2, LIU Xiao-wei 1, ZUO Shi-min 2, TONG Yun-hui 1, PAN Xue-biao 2,*, XU Jing-you 1,*
(1Horticulture and Plant Protection College, Yangzhou University, Yangzhou 225009, China; 2Key Laboratory of Plant Functional Genomics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; *Corresponding author, E-mail: shuidao@yzu.edu.cn; jyxu@yzu.edu.cn)
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Abstract According to the sequences of GenBank and relative references, a fragment of 930 bp including the total open reading frame of Ospgip1 gene was amplified. Prokaryotic expression product of the gene could inhibit the growth and polygalacturonase (PG) activity of Rhizoctonia solani, the pathogen of rice sheath blight. Bioinformatics analysis showed that OsPGIP1 was a hydrophobic protein with a molecular weight of 32.8 kDa and a pI of 7.26. The protein was mainly located in the cell wall of rice, and the splice site of its signal peptide was between the 17th and 18th amino acid residue. There were four cysteines in the N- and C-terminal of the deduced amino acid sequence, respectively, forming three disulfide bonds (Between 56th and 63rd, 278th and 298th, 300th and 308th amino acid residue, respectively). The main structural elements of the deduced protein, which showed the typical leucine-rich repeat(LRR) modular organization, were α-helix, β-sheet and irregular circle. Comparing to PGIPs of other plants, the 7th LRR of this protein was absent. The nine LRRs could form a cleft which would be the activity site domain between the protein-protein interaction of the PGIP from rice and PG from the pathogenic fungi.
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CHEN Xi-jun
LIU Xiao-wei
ZUO Shi-min
TONG Yun-hui
PAN Xue-biao
XU Jing-you
Key wordspolygalacturonase-inhibiting protein   prokaryotic expression   bioinformatics analysis   rice sheath blight   rice      
Received: 2010-06-26;
Cite this article:   
CHEN Xi-jun ,LIU Xiao-wei ,ZUO Shi-min et al. Prokaryotic Expression of Polygalacturonase-Inhibiting Protein Gene (Ospgip1) from Rice and Bioinformatics Analysis of Its Coding Product

[J]. , 2011, 25(2): 136-142 .
 
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