中国水稻科学
     Home | About Journal | Editorial Board | Publication Ethics Statement | Subscriptions | Advertisement | Contacts Us | Chinese
  2010, Vol. 24 Issue (6): 677-680     DOI: 10.3969/j.issn.1001-7216.2010.06.021
实验技术 Current Issue | Next Issue | Archive | Adv Search  |   
A Simple Method for Rapid Preparation of Rice Genomic DNA
SUN Chuan1,2, #;CHEN Gang1,2, #; RAO Yu-chun1; ZHANG Guang-heng1;GAO Zhen-yu1; LIU Jian1; JU Pei-na1; HU Jiang1; GUO Long-biao1;QIAN Qian1,*;ZENG Da-li 1,*
1 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 2 Agricultural College, Yangzhou University, Yangzhou 225009, China; # These authors contributed equally to this paper; *Corresponding authors, E-mail: qianqian188@hotmail.com; dalizeng@126.com
 Download: PDF (706 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract A simple method for preparation of rice genomic DNA was developed. A small amount (1- 50 mg) of leaf tissue of rice seedling, 500 μL of extraction buffer, and one steel bead were put into a 2mL microcentrifuge tube. After vigorously mashing for 2 min, 5 μL of the supernatant was directly applied to PCR amplification. Otherwise, the supernatant was precipitated with 2 volumes of ethanol to obtain high quality genomic DNA. This method is simple, rapid, low cost, and reliable for PCR analysis. One person can manipulate as many as 96 samples for PCR in 10 minutes. It is especially suitable for genotyping of large number of samples.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
SUN Chuan
CHEN Gang
RAO Yu-chun
ZHANG Guang-heng
GAO Zhen-yu
LIU Jian
JU Pei-na
HU Jiang
GUO Long-biao
QIAN Qian
ZENG Da-li
Key wordsDNA extraction   high-throughput PCR amplification   sample preparation   methodology   rice      
Received: 1900-01-01;
Cite this article:   
SUN Chuan,CHEN Gang,RAO Yu-chun et al. A Simple Method for Rapid Preparation of Rice Genomic DNA[J]. , 2010, 24(6): 677-680 .
 
[1] 王兰, 龙云铭, 刘耀光. 一种用于PCR的植物基因组DNA快速制备方法. 分子植物育种, 2009, 7(2): 425-428.
[2] 余守武, 刘宜柏. 分子标记在水稻遗传育种中的应用. 江西农业大学学报: 自然科学版, 2003, 25(1): 111-116.
[3] 赵红霞, 谢攀, 黄志坚, 等. 一种改良的水稻总DNA提取方法. 湖北大学学报: 自然科学版, 2006, 12(4): 389-392.
[4] Stewart C N Jr, Via L E. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechniques, 1993, 14(5): 748-750.
[5] Ausubel F M, Brent R, Kingston R E, et al. Phenol/SDS method for plant RNA preparation//Current Protocols in Molecular Biology. New York: John Wiley and Sons, Inc, 1996.
[6] 王会文, 吴琛, 赵晓瑜, 等. SDS一步法制备PCR模板. 河北大学学报: 自然科学版, 2002, 22(2): 186-188.
[7] 汪秀峰, 杨剑波, 向太和, 等. 一种叶片直接用作PCR扩增的新方法及其应用. 中国水稻科学, 2002, 16(1): 67-70. 浏览
[8] 陈文岳, 包劲松, 周祥胜, 等. 一种可用于PCR分析的水稻DNA简易提取法. 中国水稻科学, 2005, 19(6): 561-563. 浏览
[9] 邱福林, 王和和, 陈 洁, 等. 用于水稻突变体大量筛选的DNA微量快速提取法. 中国水稻科学, 2006, 20(3): 329-332. 浏览
[10] Paris M, Carter M. Cereal DNA: A rapid high-throughput extraction method for marker assisted selection. Plant Mol Biol Rep, 2000, 18(4): 357-360.
[11] Mace E S, Buhariwalla H K, Crouch J H. A high-throughput DNA extraction protocol for tropical molecular breeding programs. Plant Mol Biol Rep, 2003, 21(4): 459a-459h.
[12] Xu X, Kawasaki S, Fujimura T, et al. A protocol for high-throughput extraction of DNA from rice leaves. Plant Mol Biol Rep, 2005, 23(3): 291-295.
[13] Kamalay J, Tejwani R, Keith Rufener G. Isolation and analysis of genomic DNA from single seeds. Crop Sci, 1990, 30(5): 1079-1084.
[14] John M E. An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucl Acids Res, 1992, 20(9): 2381.
[15] Sambrook J, Russell D. 分子克隆实验指南. 3版. 黄培堂, 译. 北京: 科学出版社, 2002.
[16] Sharp P J, Kreis M, Shewry P R, et al. Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet, 1988, 75(2): 286-290.
[1] XU Jian-Jun1, 2 , ZHAO Qiang3, TANG Zai-Xiang1, ZHAO Yuan-Feng1, ZHU Lei1, XU Chen-Wu1, GU Ming-Hong1, HAN Bin3 . Mapping of QTLs for Grain Shape Using WholeGenome Resequenced  Chromosome Segment Substitution Lines in Rice[J]. , 2011, 25(4): 365-369.
[2] MA Cui, FAN Xiao-Rong*, XU Guo-Hua. Responses of  Rice Plants of Wuyunjing 7 to Nitrate as Affected by  OverExpression of OsNRT1.2[J]. , 2011, 25(4): 349-356.
[3] LI Yu-Hong1, 2 , DAI Zheng-Yuan1, LI Ai-Hong1, CHEN Xi-Jun2, WANG Bao-He1, ZHAO Bu-Hong1, LIU Guang-Qing1, PAN Xue-Biao2,ZHANG Hong-xi1,*. Role of Rice Main Parent BG902 in Breeding of Yangdao Series and  Their  Bacterial Blight  Resistance[J]. , 2011, 25(4): 439-442.
[4] XIN Ming-Yue, YIN Hong *,Zhang Tao , ZHANG Mei-Ling. Estimation of  Rice Absorbed Photosynthetically Active Radiation by  Hyperspectral Remote Sensing[J]. , 2011, 25(4): 443-446.
[5] HUANG Xing-Guo1, 2 ,WANG Guang-yong1,#, YU Jin-hong1, DING Yi1,*. Cytoplasmic Genetic Effects  and Cytological Study  of Isonuclear  Alloplasmic  Male Sterile  Lines  in Rice [J]. , 2011, 25(4): 370-380.
[6] XU Fu-Rong1, 2 , DONG Chao1, YANG Wen-Yi1, TANG Cui-Feng1, A Xin-Xiang1, ZHANG En-Lai1, YANG Ya-Yun1, ZHANG Fei-Fei1, DAI Lu-Yuan1, * , ZHANG Hong-Sheng2, * . Comparison of Genetic Diversity of  Rice Landraces Planted in Two Periods in Hani′s Terraced Fields in Yuanyang County, Yunnan  Province, China Using Microsatellite Markers[J]. , 2011, 25(4): 381-386.
[7] YU Ping, YUAN Xiao-Ping, XU Qun, WANG Cai-Hong, YU Han-Yong, WANG Yi-Ping, WEI Xing-Hua*. Genetic Structure and Indica/Japonica Component of Major Inbred Rice Varieties in China[J]. , 2011, 25(4): 387-391.
[8] ZHANG Ya-Jie, HUA Jing-Jing, LI Ya-Chao, CHEN Ying-Ying, YANG Jian-Chang*. Effects of Phosphorous  on Grain Quality of Upland  and Paddy Rice under Different Cultivations[J]. , 2011, 25(4): 399-406.
[9] LIU Guo-Kun, WANG Yu, XIAO Shun, ZHANG Shao-Sheng*. Pathogen Identification and   Infection Sources of Rice RootKnot Disease  [J]. , 2011, 25(4): 420-426.
[10] TANG Liang1, LI Yan-Da1, 2 , ZHANG Yu-Ping1, 3 , ZHU Xiang-Cheng1, LIU Xiao-Jun1, CAO Wei-Xing1, ZHU Yan1, * . Simulation of Canopy Light Distribution and Application  in Rice[J]. , 2011, 25(4): 427-434.
[11] GONG Jin-long1 , ZHANG Hong-cheng1, * , LI Jie1 , CHANG Yong1 , DAI Qi-gen1 , HUO Zhong-yang1 , XU Ke1 , WEI Hai-yan1 , LI De-jian2 , LI Bing-wei2 , SHA An-qin2 , ZHOU You-yan2 , LUO Xue-chao2 , ZHU Zhen3. Effects of Phosphorus  Levels on Grain Yield and Quality of Super Rice Nanjing 44[J]. , 2011, 25(4): 447-451.
[12] LI Peng,GE Ying,WU Long-hua,SHEN Li-bo,TAN Wei-na,LUO Yong-ming. Uptake and Translocation of Cadmium and Its Physiological Effects in Two Rice Cultivars Differed in Grain Cadmium Concentration[J]. , 2011, 25(3): 291-296 .
[13] ZHANG Shui-qing,ZHONG Xu-hua,HUANG Nong-rong,LU Guo-an. Effects of Straw Mulching on Dry Matter Production and Grain Yield of Double Cropping LateSeason Rice (Oryza sativa) in South China [J]. , 2011, 25(3): 284-290 .
[14] ZHANG Zhi-yong,WANG Dong-lan,ZHANG Cun-zheng,WU Chang-fu,LIU Xian-jin*. Difenoconazole Residues in Rice and Paddy System[J]. , 2011, 25(3): 339-342 .
[15] ZHAO Yan,LUO Yuan-yuan,ZHANG Xiao-li,GUO Long-biao,QIAN Qian,DONG Yu-ran. A Simple Selection Method for Effective Screening Concentration of NaCl for Generating Marker-Free Salt-Tolerant Transgenic Rice[J]. , 2011, 25(3): 243-248 .
[16] WU Shao-hua,XUE Jing-jing,ZHANG Hong-yu,XU Pei-zhou,WU Xian-jun*. Analysis of Special DNA Methylated Sites Between Haploid of TwinSeedling and Its Hybrids in Rice[J]. , 2011, 25(3): 249-255 .
[17] WU Chao,FU Ya-ping,HU Guo-cheng,SI Hua-min,LIU Xu-ri,SUN Zong-xiu,CHENG Shi-hua,LIU Wen-zhen. Identification and Fine Mapping of a Spotted and Yellow Leaf Mutant in Rice [J]. , 2011, 25(3): 256-260 .
[18] FANG Yun-xia,SONG Xiu-juan,PENG You-lin,DONG Guo-jun,GUO Long-biao,ZENG Da-li,ZHANG Guang-heng,YAN Hong-lan,QIAN Qian. Genetic Analysis and Molecular Mapping of a Rice Rumpled and Twisted Leaf Mutant (rtl1)[J]. , 2011, 25(3): 261-266 .
[19] ZHOU Zhen-ling,#,WEI Xiang-jin,JIANG Ling,LIU Kai,XU Da-yong,ZHAI Hu-qu,WAN Jian-min. Genetic Analysis of Heading Date of Japonica Rice Cultivars in Southwest China[J]. , 2011, 25(3): 267-276 .
[20] DONG Ming-hui,XIE Yu-lin,QIAO Zhong-ying,LIU Xiao-bin,WU Xiang-zhou,ZHAO Bu-hong,YANG Jian-chang. Variation in Carbohydrate and Protein Accumulation Between Spikelets at Different Positions Within a Rice Panicle During Grain Filling[J]. , 2011, 25(3): 297-306 .
[21] FAN Hong-huan,WANG Lin-you,ZHANG Li-xia,YU Xin,WANG Xi,JIN Qing-sheng,WANG Jian-jun . Breeding of Rice Lines with Bacterial Blight Resistance Gene Xa23 by Using MarkerAssisted Selection[J]. , 2011, 25(3): 331-334 .
[22] ZHANG Shun-tang,ZHANG Gui-lian,CHEN Li-yun ,XIAO Ying-hui . Effects of High Temperature Stress on Net Photosynthetic Rate and Chlorophyll Fluorescence Parameters of Flag Leaf in Rice[J]. , 2011, 25(3): 335-338 .
[23] PEI Qing-li ,#,WANG Chun-lian ,#,LIU Pi-qing ,WANG Jian ,ZHAO Kai-jun . Marker-Assisted Selection for Pyramiding Disease and Insect Resistance Genes in Rice[J]. , 2011, 25(2): 119-129 .
[24] GU Jian-wei ,LIU Jing ,XUE Yan-jiu ,ZANG Xin ,XIE Xian-zhi . Phytochrome Functions in Rice Development[J]. , 2011, 25(2): 130-135 .
[25] CHEN Xi-jun ,LIU Xiao-wei ,ZUO Shi-min ,TONG Yun-hui ,PAN Xue-biao ,XU Jing-you . Prokaryotic Expression of Polygalacturonase-Inhibiting Protein Gene (Ospgip1) from Rice and Bioinformatics Analysis of Its Coding Product

[J]. , 2011, 25(2): 136-142 .
[26] MA Jian-yang,CHEN Sun-lu,ZHANG Jian-hui,DONG Yan-jun ,TENG Sheng. Identification and Genetic Mapping of a Lesion Mimic Stripe Mutant in Rice[J]. , 2011, 25(2): 150-156 .
[27] JIANG Hua ,#,ZHAO Jiang-hong ,#,GUO Long-biao ,JIANG Liang ,XUE Da-wei,ZENG Da-li,QIAN Qian,SUN Guo-chang . QTL Mapping and Epistatic Analysis of High-Order Tillering in Rice (Oryza sativa)[J]. , 2011, 25(2): 157-162 .
[28] LI Sheng-qiang,CUI Guo-kun,GUAN Cheng-ran,WANG Jun,LIANG Guo-hua*. QTL Detection for Rice Grain Shape Using Chromosome Single Segment Substitution Lines[J]. , 2011, 25(2): 163-168 .
[29] LAN Yan-rong#,WANG Jun-yi#,WANG Yi,MOU Tong-min* . Improvement of Rice Bacterial Blight Resistance of Hua 201S, an Elite Photo-Thermo- Sensitive Genic Male Sterile Line, by Molecular Marker-Assisted Selection[J]. , 2011, 25(2): 169-174 .
[30] DONG Chao,XU Fu-rong ,YANG Wen-yi ,ZHANG En-lai ,YANG Ya-yun ,TANG Cui-feng ,A Xin-xiang ,DAI Lu-yuan . SSR Marker-Based Diversity Analysis on Rice Landraces Currently Planted by the Bulang
Minority in China
[J]. , 2011, 25(2): 175-181 .
[31] TANG Wen-bang,ZHANG Gui-lian,XIAO Ying-hui,DENG Hua-bing,FAN Ke,LIU Guo-hua,CHEN Li-yun*. Plant Type and Dry Matter Production Characteristics of C Liangyou Hybrid Rice
Combinations
[J]. , 2011, 25(2): 189-194 .
[32] ZHAO Feng ,# ,XU Chun-mei ,# , ZHANG Wei-jian ,ZHANG Xiu-fu ,CHENG Jian-ping ,WANG Dan-ying . Effects of Rhizosphere Dissolved Oxygen and Nitrogen Form on Root Characteristics and
Nitrogen Accumulation of Rice
[J]. , 2011, 25(2): 195-200 .
[33] ZOU Cheng-jia#,TANG Fang# ,YANG Mei,HE Xiao-xia,LI Xian-jun,ZHOU Er-xun*. Studies on Biological Characteristics and Pathogenicity Differentiation of Rice Sheath Blight Pathogen from Three Provinces in South China[J]. , 2011, 25(2): 206-202 .
[34] XU De-jin,GU Zhong-yan*,XU Guang-chun,XU Xiao-long,FAN Peng. Effects of Solution Surface Tension and Spray Methods on Deposition of Droplets on Rice Plants[J]. , 2011, 25(2): 213-218 .
[35] LIU Shou-dong,SHI Pei-jian,JIANG Xiao-dong,YAO Ke-min,HU Ning*. Model-Based Analysis on the Pollen Viabilities of Transgenic Rice B2
at Different Temperatures
[J]. , 2011, 25(2): 219-222 .
[36] YING Xing-hua,XU Xia,YANG Shi-hua*,ZHU Zhi-wei,CHEN Ming-xue,WANG Lei,CHENG Ben-yi,XIA Jun-hui. Difference of Pesticide Residue in Grains Among Rice Cultivars
[J]. , 2011, 25(2): 227-230 .
[37] ZHANG Song-bai ,LUO Han-gang ,ZHANG Qiu-dong ,ZHANG Chang-qing ,WU Zu-jian ,XIE Lian-hui . A Dwarf Disease on Rice in Hubei Province, China is Caused by Southern Rice Black-Streaked Dwarf Virus [J]. , 2011, 25(2): 223-226 .
[38] XIE Jin-shui ,SHAO Cai-hong ,TANG Xiu-ying ,SHI Qing-hua . Proteomics Analysis of Nutrient Stress Effect on Rice Leaf Senescence at Grain Filling Stage[J]. , 2011, 25(2): 143-149 .
[39] JI Ying-hua,GAO Rui-zhen,ZHANG Ye,CHENG Zhao-bang,ZHOU Tong,FAN Yong-jian,ZHOU Yi-jun. A Simplified Method for Quick Detection of Rice BlackStreaked Dwarf Virus and Southern Rice BlackStreaked Dwarf Virus
[J]. , 2011, 25(1): 91-94 .
[40] PAN Yi,LUO Li-hua,DENG Hua-bing,ZHANG Gui-lian,TANG Wen-bang,CHEN Li-yun,XIAO Ying-hui*. Quantitative Trait Loci Associated with Pollen Fertility Under High Temperature Stress at Flowering Stage in Rice
[J]. , 2011, 25(1): 99-102 .
[41] YIN De-suo,XIA Ming-yuan,LI Jin-bo,WAN Bing-liang,ZHA Zhong-ping,DU Xue-shu,QI Hua-xiong*. Development of STS Marker Linked to Rice Blast Resistance Gene Pi9 in Marker Assisted Selection Breeding
[J]. , 2011, 25(1): 25-30 .
[42] GUO Shi-wei,WANG Yong-fei,MA San-mei,LI Xia,GAO Dong-ying. Genetic Analysis and Fine Mapping of a GreenRevertible Albino Leaf Mutant in Rice[J]. , 2011, 25(1): 95-98 .
[43] LIN Hong-xin,PAN Xiao-hua,SHI Qing-hua,PENG Chun-rui,WU Jian-fu,LEI Xiang-liang . Effects of RowSpacing on Yield of SuperHighYielding Early and Late Rice[J]. , 2011, 25(1): 79-85 .
[44] ZHANG Ding-ding,ZOU Li-fang,ZHAO Mei-qin,ZOU Hua-song,CHEN Gong-you. hrcQ Gene Determines Xanthomonas oryzae pv. oryzicola to Trigger Hypersensitive Response in Nonhost Tobacco and Pathogenicity in Host Rice[J]. , 2011, 25(1): 11-18 .
[45] CHEN Shu-lin,WANG Hai-yan,DING Zhen-qian,WANG Xiu-e,CHEN Zhong-ming. Construction of Molecular Fingerprinting Database and Dual PCR Assay for Two Indica Rice Hybrids and Two Japonica Rice Varieties by Using SSR Markers
[J]. , 2011, 25(1): 19-24 .
[46] ZHU Yi-yong,ZENG Hou-qing,DI Ting-jun,XU Guo-hua,SHEN Qi-rong*. Investigation on the Mechanism of Adaption of Plasma Membrane H+ATPase to Ammonium Nutrition in Rice[J]. , 2011, 25(1): 112-118 .
[47] PAN Jian-hong,CHEN Feng,HE Jia-chun,LAI Feng-Xiang,FU Qiang*. Comparison on Electrical Penetration Graph (EPG) of Two Populations of Nilaparvata lugens with Different Virulence[J]. , 2011, 25(1): 86-90 .
[48] YANG Ya-chun,NI Da-hu,SONG Feng-shun,LI Ze-fu,YI Cheng-xin,YANG Jian-bo. Identification of QTLs for Rice Appearance Quality Traits Across Different Ecological Sites[J]. , 2011, 25(1): 43-51 .
[49] FENG Yue-hua,ZOU Ying-bin,Roland J. BURESH. Effects of No-Tillage Cultivation on Some Population Characteristics of TwoLine Hybrid Rice Liangyoupeijiu [J]. , 2011, 25(1): 65-70 .
[50] YE Shi-chao,#,LIN Zhong-cheng,#,DAI Qi-gen,JIA Yu-shu,GU Hai-yan,CHEN Jing-dou,XU Lu-sheng,WU Fu-guan,ZHANG Hong-cheng,HUO Zhong-yang,XU Ke,WEI Hai-yan. Effects of Nitrogen Application Rate on Ammonia Volatilization and Nitrogen Utilization in Rice Growing Season[J]. , 2011, 25(1): 71-78 .
[51] GAO Qing-song,ZHANG Dan,XU Liang,XU Chen-wu* . Systematic Identification of Rice ABC1 Genes and Their Expression Analysis under Abiotic Stresses [J]. , 2011, 25(1): 1-10 .
[52] LI Rui,ZHAO Shu-li,MAO Ting,XU Zheng-jin ,CHEN Wen-fu. QTL Analysis on Stomatal Trait of Flag Leaf in Rice [J]. , 2010, 24(6): 659-62 .
[53] LU Yong-liang,PENG Yu-fa,WANG Wei-xia,SUN Xing-qiang,CHEN Li-juan,YU Liu-qing. Frequency of Gene Flow from HerbicideResistant Transgenic Rice to Conventional Rice Varieties
[J]. , 2010, 24(6): 663-666 .
[54] WANG Ling,HUANG Wen-wen,HUANG Shi-wen,LIU Lian-meng,LIU En-yong,. Pathogenicity Differentiation of Rice Sheath Blight Pathogen Rhizoctonia solani AG-1 ⅠA Isolates from Anhui and Hubei Provinces, China[J]. , 2010, 24(6): 623-629 .
[55] YANG Ying-qing#,YANG Mei#,LI Ming-hai,LI Yong,HE Xiao-xia,ZHOU Er-xun*. Establishment of Agrobacterium tumefaciensMediated Transformation System for Rhizoctonia solani AG-1 ⅠA, the Causal Agent of Rice Sheath Blight[J]. , 2010, 24(6): 617-622 .
[56] NING Yong-qiang,DING Wo-na,ZHU Shi-hua*,YU Hong-wei,YU Hong,LU Kai-xing. Gnetic Analysis and Gene Mapping of a Short Root Mutant ksr1 in Rice[J]. , 2010, 24(6): 652-654 .
[57] CHEN Xi-feng,GU Zhi-min,LIU Feng,MA Bo-jun,ZHANG Hong-sheng. Molecular Analysis of Rice CIPKs Involved in Biotic and Abiotic Stress Responses[J]. , 2010, 24(6): 567-574 .
[58] LIU Wu-ge ,WANG Feng ,XIAO Han-xiang ,LIU Zhen-rong,LI Jin-hua ,LIAO Yi-long ,ZHU Man-shan ,HUANG Hui-jun,HUANG Bing-chao ,ZHANG Yang. Improvement of Gall Midge Resistance for Restorer Line in Rice by Molecular
Marker Assisted Selection
[J]. , 2010, 24(6): 581-586 .
[59] ZHANG Hong-lin,CHENG Jian-feng,LIU Yue-qing,ZHONG Xiao-ying,ZHANG Rui-xiang,LIU Hai-ping,ZHANG Pu . Creation and Characteristics of Indica Type Cytoplasmic Male Sterile Rice Line Gaoguang A Labeled with AlbinotoZebra Leaf Marker[J]. , 2010, 24(6): 587-594 .
[60] ZUO Jiao,QIANG Sheng,SONG Xiao-ling* . Fitness of Progenies Between Transgenic Rice and Weedy Rice under Greenhouse Conditions[J]. , 2010, 24(6): 608-616 .
[61] CHEN Li-yun,LEI Dong-yang,TANG Wen-bang,XIAO Ying-hui. Thoughts and Practice of Some Problems about Research and Application of TwoLine Hybrid Rice[J]. , 2010, 24(6): 641-646 .
[62] GAO Ju-sheng,CAO Wei-dong,DONG Chun-hua,XU Ming-gang,ZENG Xi-bai,WEN Shi-lin,.

Effects of Long-Term Rice-Rice-Green Manure Rotation on Rice Yield

[J]. , 2010, 24(6): 672-676 .
[63] YU Chu-lang,YANG Yong,WANG Xu-ming,YAN Cheng-qi,CHEN Jian-ping*. Recent Advances in Proteomic Studies on Rice Pathogen Interactions [J]. , 2010, 24(6): 647-651 .
[64] SHENG Xian-qiao,ZHANG Fa-cheng,XU Hong-xing,ZHENG Xu-song,CHEN Gui-hua,LU Zhong-xian.

Resistance Performance of Rice Varieties (Combinations) to Brown Planthopper,Nilaparvata lugens, in Paddy Field

[J]. , 2010, 24(5): 535-538 .
[65] ZHANG Ji-wen. Progress on the Study of the Bentazon Sensitive Mutants in Rice [J]. , 2010, 24(5): 551-558 .
[66] SHEN Wei-wei,SONG Cheng-li,CHEN Jie,FU Ya-ping,WU Jian-li,JIANG Shao-mei. Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance[J]. , 2010, 24(5): 447-452 .
[67] WANG Yan-rong,#,DAI Gui-jin,#,Ryu OHSUGI,Tohuru YAMAGISHI,CHENG Shi-hua. QTL Analysis on Nitrogen Uptake and Related Traits at Seedling Stage in Rice[J]. , 2010, 24(5): 463-468 .
[68] XU Qun,YUAN Xiao-ping,YU Han-yong,WANG Yi-ping,TANG Sheng-xiang,WEI Xing-hua* . Mapping QTLs for Drought Resistance at Seedling Stage in Rice by Using a Doubled Haploid Population[J]. , 2010, 24(5): 469-473 .
[69] SONG Ai-mei,HUANG Xin-peng,SUN Shu-bin,ZHANG Ya-li,XU Guo-hua*. Screening and Identification of Rice Cultivars with Relatively High Nitrogen Use Efficiency for Tolerance to Phosphorus Deficiency at Seedling Stage[J]. , 2010, 24(5): 479-486 .
[70] MAO Ting,XU hai,GUO Yan-hua,ZHU Chun-jie,CHEN Kai,WANG Jia-yu,JIANG Shu-kun,XU Zheng-jin*. Relationship Between Subspecies Differentiation and Rice Quality Traits in RILs Population from IndicaJaponica Cross
[J]. , 2010, 24(5): 474-478 .
[71] ZHANG Yu-zhu,LIU Yang ,ZENG Xiang ,CHEN Kai-lin,HUANG Ze-hui ,XIE Hong-ke . Effects of Mulching Mode on Canopy Physiological, Ecological Characteristics and Yield of Rice under Dry Cultivation [J]. , 2010, 24(5): 487-492 .
[72] XIAO Dong-lai,JIA Dong-sheng,WU Jian-guo,DU Zhen-guo,XIE Li-yan,WU Zu-jian*,XIE Lian-hui* . Interaction Between Rice Stripe Virus NS3 Protein and Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) of Rice [J]. , 2010, 24(5): 493-496 .
[73] GUAN Ze-xin,TANG Jian,YANG Bao-jun,ZHOU Ying-feng,FAN De-yao,YAO Qing . Study on Recognition Method of Rice Disease Based on Image
[J]. , 2010, 24(5): 497-497~502 .
[74] PENG Dai-liang,HUANG Jing-feng,SUN Hua-sheng,WANG Fu-min. County Level Rice Yield Estimation Based on Combination of Terra and Aqua MODIS EVIs [J]. , 2010, 24(5): 516-522 .
[75] QIAN Jun-chao,LUO Qiong*,WANG Yun-yue*,LU Jian-huan,ZHAO Guang-juan,LUO Yan. Investigation of Gene Flow Between Different Rice Varieties under Interplanting [J]. , 2010, 24(5): 527-530 .
[76] ZENG Yu-xiang,LI Xi-ming,MA Liang-yong,JI Zhi-juan,YANG Chang-deng*. Research Progress on Mapping of Gene Conferring Resistance to Sheath Blight and Exploitation of Resistance Resources in Rice[J]. , 2010, 24(5): 544-550 .
[77] XU Zheng-jin,HAN Yong,SHAO Guo-jun,ZHANG Xue-jun,QUAN Cheng-zhe,PAN Guo-jun,YAN Ping,CHEN Wen-fu. Comparison of Rice Quality Characters in Northeast Region of China[J]. , 2010, 24(5): 531-534 .
[78] HUANG Guo-ping,WANG Lin,CHEN Ke-ping . A Review on Detection Technologies for Exogenous Proteins in Transgenic Rice[J]. , 2010, 24(4): 410-416 .
[79] GONG Jin-long,ZHANG Hong-cheng,LI Jie,DAI Qi-gen,HUO Zhong-yang,XU Ke, ,LI De-jian,HUA Zheng-xiong,SHA An-Qin,ZHOU You-yan,LUO Xue-chao, LIU Guo-lin. Progress in Research on Patterns and Systematic Theories of SuperHighYielding Cultivation in Rice[J]. , 2010, 24(4): 417-424 .
[80] ZHOU Tong#,WU Li-juan#,WANG Ying,CHENG Zhao-bang,JI Ying-hua,FAN Yong-jian,ZHOU Yi-jun*. Preliminary Report on the Transmission of Rice BlackStreaked Dwarf Virus from Frozen Infected Leaves to Rice by Insect Vector Small Brown Planthopper (Laodelphax striatellus[J]. , 2010, 24(4): 425-428 .
[81] YANG Yan-hua,ZHANG Ya-dong,ZHU Zhen,ZHAO Ling,CHEN Tao,ZHAO Qing-yong,WANG Cai-lin* . Effects of Gibberellic Acid 3 and Abscisic Acid on Growth, Physiological Characteristics and Gene Expression of GA20ox2 and GA3ox2 in Different Rice Cultivars [J]. , 2010, 24(4): 433-437 .
[82] TAN Xin-zhong,PENG Xi-xu,HU Yao-jun,TANG Xin-ke,ZHOU Ping-lan,DENG Xiao-bo,WANG Hai-hua*. Relations Between Changes in Contents of Phenolic Acids and Phenolic Polymers and Induced Resistance to Bacterial Blight in Rice under Nickel Treatment[J]. , 2010, 24(4): 438-442 .
[83] WANG Bao-ju,XU Hong-xing,ZHENG Xu-song,FU Qiang,LU Zhong-xian. Effects of Temperature on Resistance of Rice to Brown Planthopper, Nilaparvata lugens [J]. , 2010, 24(4): 443-446 .
[84] LI Wei-guo, LI Hua,. Estimating Rice Yield by HJ-1A Satellite Images [J]. , 2010, 24(4): 385-390 .
[85] LI Hai-jun,LI Yong,YANG Xiu-xia,SHEN Qi-rong,GUO Shi-wei*. Effects of Different Nitrogen Forms and Water Stress on the Growth and Osmotic Adjustment of Rice Seedlings [J]. , 2010, 24(4): 403-409 .
[86] BAI Xiao-juan,LIU Li-juan,ZHANG Chun-hua,GE Ying,CHENG Wang-da. Effect of H2O2 Pretreatment on Cd Tolerance of Different Rice Cultivars[J]. , 2010, 24(4): 391-397 .
[87] LIU Lian-meng,WANG Ling,HUANG Wen-wen,LIU En-yong, HUANG Shi-wen*. Cloning, Expression and Sequence Analysis of G Protein β Subunit Gene of Rice False Smut Pathogen Ustilaginoidea virens[J]. , 2010, 24(4): 353-359 .
[88] WANG Wei,WU Chao,LIU Mei,LIU Xu-ri,HU Guo-cheng, SI Hua-min, SUN Zong-xiu, LIU Wen-zhen,FU Ya-ping. Resistance of Antimicrobial Peptide Genes Transgenic Rice to Bacterial Blight[J]. , 2010, 24(4): 335-340 .
[89] YAO Shu,CHEN Tao,ZHANG Ya-dong,ZHU Zhen,ZHAO Ling,ZHAO Qing-yong,ZHOU Li-hui,WANG Cai-lin. Pyramiding of Translucent Endosperm Mutant Gene Wx-mq and Rice Stripe Disease Resistance Gene Stv-bi by MarkerAssisted Selection in Rice (Oryza sativa) [J]. , 2010, 24(4): 341-347 .
[90] LIU Xi-ling, ZOU Hua-song,ZOU Li-fang, CHEN Gong-you. Site-Directed Mutagenesis and Functional Analysis of hrcU Gene from Rice Pathogen Xanthomonas oryzae pv. oryzae [J]. , 2010, 24(4): 348-352 .
[91] LIU Bo, HU Gui-ping, ZHENG Xue-fang, ZHANG Jian-fu, XIE Hua-an . Analysis on Microbial Diversity in the Rhizosphere of Rice by Phospholipid Fatty Acids Biomarkers [J]. , 2010, 24(3): 278-288 .
[92] LIU Xiao-jun,CAO Jing,TANG Liang,CAO Wei-xing,ZHU Yan*. Development and Application of a Model and GISBased Decision Support System for Rice Production Management[J]. , 2010, 24(3): 297-302 .
[93] DING Wei-long,LIU Chang,ZHANG Yu-ping,ZHU De-feng,ZHANG Qian-yuan,GU Hui . Visually Modeling Spatial Shape of Rice Panicle Based on Calculation of Bending Deformation[J]. , 2010, 24(3): 309-314 .
[94] MA Lin,YU Xian-quan*,ZHAO Fu-sheng. SSR-Based Analysis on Genetic Diversity of Rice Landraces from Guizhou Province, China [J]. , 2010, 24(3): 237-243 .
[95] LIANG Yun-tao,#,WANG Chun-lian,#, LAI Feng-xiang, LIU Pi-qing,WANG Jian,FU Qiang,ZHAO Kai-jun. Development of STS Marker KC1 for Brown Planthopper Resistance Gene Bph18(t) and Marker Assisted Selection Efficiency[J]. , 2010, 24(3): 244-250 .
[96] DI Yu-ting,ZHAO Guo-chen,XU Ke-zhang,WU Zhi-hai,ZHANG Zhi-an,LING Feng-lou,JIANG Nan. Changes in Organ Biomass in Rice Cultivars Released by Jilin Province, China During FortySeven Years of Genetic Improvement[J]. , 2010, 24(3): 251-256 .
[97] MENG Jie,WANG Ren-min*,WAN Ji-li,FU Li-cheng . Responses of Seedling Growth and Antioxidase Activities of Different Zinc Efficient Rice Genotypes and Their F1 Hybrids to Zn2+ Activities [J]. , 2010, 24(3): 289-296 .
[98] SHI Chun-lin,FENG Hui-hui,JIN Zhi-qing, WANG Hua. Comparison of Phasic Development Models in Rice[J]. , 2010, 24(3): 303-308 .
[99] LIN Zhong-cheng,#,YE Shi-chao,#, DAI Qi-gen,CHEN Jing-dou,GU Hai-yan,ZHAO Hong-xiang,ZHAO Xiao-hua, ZHANG Hong-cheng,HUO Zhong-yang,XU Ke, WEI Hai-yan . Effects of Located Nitrogen Application and Nitrogen Application After Exhaustion on Rice Yield and Nitrogen Use Efficiency in the Taihu Lake Region, China[J]. , 2010, 24(3): 271-277 .
[100] ZHENG Wen-jing,LIU Zhi-heng,ZHANG Yan-zhi,LIU Xin,WANG Chang-hua,ZHAO Jia-ming. Analysis of Genetic Model for Rice Stripe Disease Resistance in Rice Restorer Line C224[J]. , 2010, 24(3): 228-232 .
Copyright © Chinese Journal of Rice Science 浙ICP备05004719号-5
Supported by: Beijing Magtech