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  2010, Vol. 24 Issue (6): 567-574     DOI: 10.3969/j.issn.1001-7216.2010.06.002
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Molecular Analysis of Rice CIPKs Involved in Biotic and Abiotic Stress Responses
CHEN Xi-feng1,2;GU Zhi-min2;LIU Feng2; MA Bo-jun2;ZHANG Hong-sheng1,*
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; *Corresponding author, E-mail:
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Abstract Plant calcineurin Blike (CBL) proteins have been proposed as important Ca2+ sensors and specifically interact with CBLinteracting protein kinases (CIPKs) in plantspecific calcium signaling. We identified and isolated 15 CIPK genes in japonica rice variety Nipponbare based on the predicted sequences of rice CIPK gene family. Gene structure analysis showed that these 15 genes were divided into intronless and intronrich groups, in which OsCIPK3 and OsCIPK24 exhibit alternative splicing. The phylogenetic analysis indicated that rice CIPKs share an ancestor with Arabidopsis and poplar CIPKs. Analysis of gene expression showed that these OsCIPK genes were differentially induced by biotic stress (bacterial blight, BB) and abiotic stresses, including heavy metal (Hg2+), high salt, cold and ABA. Interestingly, five OsCIPK genes (OsCIPK1, OsCIPK2, OsCIPK10, OsCIPK11 and OsCIPK12) were transcriptionally upregulated after BB infection, among which four OsCIPK genes (OsCIPK2, OsCIPK10, OsCIPK11 and OsCIPK14) were induced by all treatments, indicating that some of OsCIPKs were involved in multiple stress response pathways in plants. The findings suggest that CIPKs play a key role in both biotic and abiotic stress responses.
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CHEN Xi-feng
GU Zhi-min
LIU Feng
MA Bo-jun
ZHANG Hong-sheng
Key wordsrice   CIPK family   gene expression   stress   bacterial blight     
Received: 1900-01-01;
Cite this article:   
CHEN Xi-feng,GU Zhi-min,LIU Feng et al. Molecular Analysis of Rice CIPKs Involved in Biotic and Abiotic Stress Responses[J]. , 2010, 24(6): 567-574 .
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Resistance Performance of Rice Varieties (Combinations) to Brown Planthopper,Nilaparvata lugens, in Paddy Field

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