Chinese Journal of Rice Science

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Identification of a Propiconazol Inducible Gene in Rice

YIN De-suo , WANG Shi-quan , DENG Qi-ming , LI Shuang-cheng , WANG Ling-xia , LI Ping
  

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10

一个丙环唑诱导的水稻基因cDNA片段的分离与分析

殷得所1,2;王世全1,2;邓其明1,2;李双成1,2 ;王玲霞1,2; 李平1,2,*   

  1. 四川农业大学 水稻研究所, 四川 成都 611130; 2四川农业大学 西南作物基因资源与遗传改良教育部重点实验室, 四川 雅安 625014;*通讯联系人, E-mail:liping@cngk.com

Abstract: After rice seedlings were induced by spraying the fungicide propiconazole,a cDNA fragment was cloned by(mRNA) differential display,whose corresponding transcription was enhanced in response to chemical treatment.Bioinformatics analysis revealed that this cDNA was homologous to multiple ESTs of rice and its amino acid sequences was homologous to GMPase gene in several plants including rice on chromosome 1 with 100% identities.RT-PCR showed that the transcript level increased after application of exogenous propiconazole.

Key words: rice, chemical induction, mRNA differential display, GDP-mannose pyrophosphorylase gene, propiconazol

摘要: 采用差异显示技术,以丙环唑作为诱导剂,对水稻幼苗进行筛选,得到一个化学诱导表达增强的差异片段。生物信息学分析表明,此片段可在水稻EST库中找到多个同源序列,其氨基酸序列与水稻、拟南芥等植物的GMPase基因高度同源,位于水稻第1染色体,是抗坏血酸合成途径中的关键酶。RTPCR实验表明此基因在丙环唑诱导后表达增强。

关键词: 水稻, 化学诱导, mRNA差异显示, 鸟苷甘露糖焦磷酸化酶基因, 丙环唑