Abstract:

BTB/POZ domain is localized in N-terminal region of this protein family,which belongs to C2H2 zinc finger transcriptional factors.A protein encoded by GMPOZ gene contained this domain was localized in cell nucleus.A gene(OsBTB) coding for a GMPOZ-like protein detected in the near-isogenic rice(Oryza sativa L.subsp.indica) lines,H7R and H7S,infected with an isolate of Magnaporthe grisea ZB15,was sequenced and characterized.Northern and Western blotting assay indicated that OsBTB was induced during M.grisea infection in the rice isogenic lines H7R and H7S,and expressed differentially in the incompatible and compatible interaction between rice and M.grisea.Quantitative RT-PCR results indicated representation of OsBTB gene expression was corresponding with the selected resistant and pathogenesis-related genes.Thought together,it might be involved in resistant reaction induced by M.grisea.

摘要：

报道了1个从稻瘟病菌诱导的水稻近等基因系H7R/H7S中克隆的类GMPOZ基因——OsBTB，通过测序获其全长cDNA并分析了该基因的序列特征。根据水稻基因组序列数据库搜索，将OsBTB基因定位在水稻基因组第2染色体上。Northern和Western杂交结果表明，OsBTB基因在水稻近等基因系H7R/H7S中均受稻瘟病菌诱导表达，且在非亲和与亲和互作中表达差异明显。定量PCR结果表明该基因的表达模式与所选取的抗性及抗性相关基因的表达模式一致。综合结果提示该基因可能参与了稻瘟病菌诱导的抗性反应。