Chinese Journal of Rice Science

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Purification and Analysis of Abscisic Acid-Specifically-Inducible Protein from Rice Callus

JIANG Hua , WAN Jia , GAO Xiao-ling , CHEN Jing , WANG Xu-dong , XU Zheng-jun
  

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10

水稻愈伤组织中脱落酸特异诱导蛋白的纯化及分析

姜华1,2;万佳1;高晓玲1;陈静1;汪旭东1,*;徐正君1,3,*   

  1. 1四川农业大学 水稻研究所, 四川 温江 611130; 2山东轻工业学院 食品与生物工程学院, 山东 济南 250353; 3四川农业大学 西南作物基因资源与遗传改良教育部重点实验室, 四川 雅安 625014; * 通讯联系人, E-mail: Zhengjunxu@hotmail.com

Abstract: Two ABA-specifically-inducible proteins from rice callus,were isolated and purified by precipitation with 65%-100% saturated(NH4)2SO4,followed by the DEAE-sepharose,TSK-gel,and two-dimension electrophoresis.Iso-electric point(pI) of the proteins with the same molecular mass(24.5 kD)were 6.1 and 6.9 respectively.Western blotting analysis indicated that the expression of the proteins at the different tissues had obvious diversity.The A1(pI 6.1) protein was(detected) in the rice callus treated with ABA and the seed embryos(SE),but not in young plants,the callus without ABA treatment and the white dry callus(WDC).The A2(pI 6.9) protein was found in the callus treated with ABA,WDC and SE,but not in other materials without ABA treatment.These two proteins might play some important roles in the processes of seed embryo(or somatic embryos) formation.

Key words: rice, callus, abscisic acid, specifically-inducible protein, purification, expression

摘要: 用硫酸铵分级沉淀、离子交换(DEAESepharose和TSKgel)的方法,结合SDSPAGE和2D电泳(twodimension electrophoresis)技术,从水稻愈伤组织中分离并精制了两个分子量相同(24.5 kD), 等电点分别为61和6.9的脱落酸(ABA)特异诱导蛋白。Westernblot印迹分析发现,这两个蛋白在不同组织中的表达差异明显。A1(pI 6.1)蛋白在ABA处理的水稻愈伤组织和种子胚中表达,但在未经ABA处理的材料(包括幼芽、愈伤组织)和白色干燥性愈伤组织中没有检测到表达;A2(pI 6.9)蛋白在经ABA处理材料、种胚和白色干燥性愈伤组织中检测到表达,在未经ABA处理的材料中没有检测到表达。推测这两个蛋白可能在胚(或不定胚)的形成过程中起重要作用。

关键词: 水稻, 愈伤组织, 脱落酸, 特异诱导蛋白