Relationship Between Protein Composition and Total Protein Content and Starch RVA Profile Properties in Rice

doi:10.3969/j.issn.1001-7216.2009.04.13

Abstract

Abstract: A recombinant inbred line (RIL) population derived from the cross between two japonica rice, W1721 with low glutelin content (LGC) and Lunhui 422 with normal glutelin content, was developed to investigate the effect of LGC gene on RVA profile properties and relationship between total protein content and starch RVA profile properties. According to SDSPAGE band type, genotypes of individuals at LGC locus were estimated: LGCLGC with low glutelin content and high prolamin content, lgclgc with high glutelin content and low prolamin content in the RIL population. The RVA profile characteristics of defatted rice showed breakdown and consistence values of LGCLGC were extremely significantly higher than those of lgclgc. The LGC gene exhibited a highly significant relative additive effect on consistence value (4.45%), explaining 12.81% of the phenotypic variance, whereas the LGC exhibited a significant relative additive effect on breakdown value (3.91%), explaining 481% of the phenotypic variance. For the genotype LGCLGC, total protein content had a negative and positive linear regression relationship with breakdown value (P<0.045) and setback value (P<0.030), explaining 9.86% and 11.48% of the phenotypic variance, respectively, whereas for the genotype lgclgc, total protein content had a negative linear regression relationship with setback value (P<0.006) and consistence value (P<0.000), explaining 13.41% and 27.88% of the phenotypic variance, respectively. Combined with previous studies, it is deduced that eating quality of rice is determined, at least in part, by both the relative proportions of the two major protein fractions, glutelin and prolamin, and total protein content. Effect of total protein content on eating quality of rice varied with the relative proportions of glutelin and prolamin. Therefore, it may be possible to develop rice with desired levels of eating quality by breeding varieties with particular proportions of glutelin and prolamin and appropriate total protein content.

Key words: rice, eating and cooking quality, glutelin, prolamin, protein content, starch viscosity

摘要： 利用低谷蛋白含量（low glutelin content， LGC）粳稻品种W1721与大面积应用的杂交粳稻恢复系轮回422构建了重组自交系群体。根据种子总蛋白质的SDSPAGE带型，推断各单株LGC位点的基因型。在排除脂肪的影响下，比较了基因型LGCLGC（谷蛋白含量较醇溶蛋白低）与基因型lgclgc（谷蛋白含量较醇溶蛋白高）在RVA谱特征值上的差异，以及同一基因型内总蛋白质含量与淀粉RVA谱特征值的关系。结果表明基因型LGCLGC的崩解值和回复值的平均值分别显著和极显著大于基因型lgclgc；LGC对崩解值和回复值的相对加性效应分别为3.91%和4.45%，贡献率分别为4.81%和12.81%。LGCLGC型的稻米，总蛋白质含量与崩解值和消减值分别存在显著的负线性回归和正线性回归关系，贡献率分别为9.86%和11.48%；lgclgc型的稻米，总蛋白质含量与消减值和回复值都存在极显著的负线性回归关系，贡献率分别为1341%和27.88%。结合前人研究认为，稻米食味品质受谷蛋白相对于醇溶蛋白的含量以及总蛋白质含量的影响；而总蛋白质含量对食味品质的影响因谷蛋白相对于醇溶蛋白的含量的不同而异。因此，育种工作中应当把谷蛋白相对于醇溶蛋白的含量以及总蛋白质含量同时作为选择指标。

关键词: 水稻, 蒸煮食味品质, 谷蛋白, 醇溶蛋白, 蛋白质含量, 淀粉黏滞性谱

WU Hongkai,LIU Shijia,JIANG Ling,ZHANG Wenwei,WANG Yihua,REN Yulong,HAN Xiaohua,LIU Feng. Relationship Between Protein Composition and Total Protein Content and Starch RVA Profile Properties in Rice [J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2009.04.13 .

吴洪恺,刘世家,江玲,张文伟,王益华,任玉龙,韩小华,刘峰. 稻米蛋白质组分及总蛋白质含量与淀粉RVA谱特征值的关系[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2009.04.13 .

References

[1]莫惠栋. 我国稻米品质的改良. 中国农业科学, 1993, 26(4): 8-14.

[2]Ong M H， Blanshard J M V. Texture determinants in cooked, parboiled rice. J Cereal Sci, 1995, 21: 251-260.

[3]向远鸿, 唐启源, 黄燕湘. 稻米品质性状相关性研究： Ⅰ.籼型粘稻食味与其他米质性状的关系. 湖南农学院学报, 1990, 16(4): 325-330.

[4]李贤勇, 王元凯, 王楚桃. 稻米蒸煮品质与营养品质的相关性分析. 西南农业学报, 2001, 14(3): 21-24.

[5]舒庆尧，吴殿星，夏英武，等. 稻米淀粉RVA谱特征与食用品质的关系. 中国农业科学， 1998， 31: 25-29.

[6]隋炯明, 李欣, 严长杰, 等. 稻米淀粉RVA谱特征与品质性状相关性研究. 中国农业科学, 2005, 38: 657-663.

[7]张小明, 石春海, Katsvra T. 粳稻米淀粉特性与食味间的相关性分析. 中国水稻科学, 2002, 16(2): 157-161.

[8]沈鹏, 罗秋香, 金正勋. 稻米蛋白质与蒸煮食味品质关系研究. 东北农业大学学报, 2003, 34(4): 368-371.

[9]刘建, 魏亚凤, 徐少安. 水稻生育中期氮肥施用与稻米蛋白质含量及淀粉黏滞性的关系. 江苏农业学报, 2005, 21(2): 80-85.

[10]Tan Y F, Corke H. Factor analysis of physicochemical properties of 63 rice varieties. J Sci Food & Agric, 2002, 82: 745-752.

[11] Villareal C P, Juliano B O. Waxy gene factor and residual protein of rice starch granules. Starch, 1986, 38: 118-123.

[12] Katsube T, Kurisaka N, Ogawa M, et al. Accumulation of soybean glycinin and its assembly with the glutelins in rice. Plant Physiol, 1999， 120: 1063-1073.

[13]孙平. 蛋白质含量多会降低稻米的食味吗？试析日本产销界关于稻米食味和应否追肥问题的争议. 中国稻米, 1998（5）: 31-33.

[14]杨静, 罗秋香, 钱春荣, 等. 氮素对稻米蛋白质组分含量及蒸煮食味品质的影响. 东北农业大学学报, 2006, 37(2): 145-150.

[15]江绍玫, 朱速松, 刘世家, 等. 水稻谷蛋白突变体的筛选及遗传分析. 遗传学报, 2003, 30(7): 641-645.

[16]Wang Y H, Liu S J, Ji S L, et al. Fine mapping and markerassisted selection of a low glutelin content gene in rice. Cell Res, 2005, 15(8): 622630.

[17]毕京翠, 张文伟, 肖应辉, 等. 应用近红外光谱技术分析稻米蛋白质含量. 作物学报, 2006, 32(5): 709-715.

[18]American Association of Cereal Chemists (AACC). Methods 6102 for RVA. 9th edn. St. Paul, MN: AACC, 1995.

[19]吴洪恺, 梁国华, 顾燕娟, 等. 水稻淀粉合成相关基因对稻米RVA谱特征的影响. 作物学报, 2006, 32(11): 1597-1603.

[20]陈能, 罗玉坤, 谢黎虹, 等. 我国水稻品种的蛋白质含量及与米质的相关性研究. 作物学报, 2006, 32(8): 1193-1196.

[21]Baxter G, Blanchard C, Zhao J. Effects of prolamin on the textural and pasting properties of rice flour and starch. J Cereal Sci, 2004, 40: 205-211.

[22]Chrastil J. Proteinstarch interactions in rice grains: Influence of storage on oryzenin and starch. J Agric & Food Chem, 1990, 38: 1804-1809.

[23]蒋冬花, 黄大年, 任不凡, 等. 水稻醇溶蛋白总含量的多态性分析. 中国粮油学报, 2007, 22(1): 5-8.