Chinese Journal of Rice Science

• 研究报告 • Previous Articles     Next Articles

QTL Analysis of Cold Tolerance at the Booting Stage in Yunnan Rice Variety Chongtui

YE Chang-rong; KATO Akira; SAITO Koji; ISE Kazuo; DAI Lu-yuan; YANG Qin-zhong   

  • Received:1900-01-01 Revised:1900-01-01 Online:2001-01-10 Published:2001-01-10

云南稻种冲腿的孕穗期耐冷性QTL分析

叶昌荣1; 加藤 明2; 齐藤 浩二2; 伊势 一男3; 戴陆园1; 杨勤忠1   

  1. 1云南省农业科学院 农作物品种资源站,云南 昆明 650205;E-mail:yechangr@public.km.yn.cn;2 北海道农业试验场,日本 札幌 062;3日本国际农林水产业研究中心,日本 筑波 305

Abstract: The restriction fragment length polymorphism (RFLP) between Japanese variety (Norin 20) and Yunnan variety (Chongtui) were analyzed with 352 markers in this study. One hundred and fifty seven markers had polymorphous bands between the two varieties were detected. Fifty five selected RFLP markers were used for the quantitative trait loci (QTL) analysis of the cold tolerance at booting stage of 70 F 3 lines of the cross of Norin 20/Chongtui. The results showed that some loci are related to the cold tolerance. The QTLs are mainly distributed on chromosome 1,3,4,5,6,7,8,10 and 12 with major ones on chromosome 3 and chromosome 7.

Key words: restriction fragment length polymorphism, quantitative trait loci, cold tolerance, booting stage, rice

摘要: 利用352 个探针分析了日本品种农林20和云南品种冲腿之间的限制性片段长度多态性(RFLP),从中筛选出157个在农林20和冲腿间显示多态性的探针,其中55个被用于其杂交后代(农林20/冲腿)的70个F3系统的孕穗期耐冷性数量性状位点(QTL)分析。结果初步显示出一些与水稻孕穗期耐冷性有关的QTL,它们主要分布在第1、3、4、5、6、7、8、10和第12染色体上。可能在第3和第7染色体上具有对孕穗期耐冷性作用较大的QTL。

关键词: 限制性片段长度多态性, 数量性状位点, 耐冷性, 孕穗期