Chinese Journal of Rice Science ›› 2015, Vol. 29 ›› Issue (3): 305-310.DOI: 10.3969/j.issn.1001-7216.2015.03.010

• Research Papers • Previous Articles     Next Articles

Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/zt

HUA  Lixia 1,2,# , WANG Wenjuan 1,2,# , CHEN Shen1,2 , WANG Congying 1,2 , ZENG Liexian1,YANG Jianyuan1, ZHU Xiaoyuan1,  SU Jing 1,2,*   

  1. 1 The Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 2Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China
  • Received:2014-05-21 Revised:2014-07-15 Online:2015-05-10 Published:2015-05-10
  • Contact: SU Jing1,2,*

抗稻瘟病Pi2/9/z-t基因特异性分子标记的开发

华丽霞1,2, # ,汪文娟1,2,# ,陈深1,2,汪聪颖1,2,曾烈先1,杨健源1,朱小源1,苏菁1,2,*   

  1. 1 广东省农业科学院 植物保护研究所, 广州 510640; 2 广东省植物保护新技术重点实验室, 广州 510640
  • 通讯作者: 苏菁1,2,*
  • 基金资助:

    国家自然科学基金资助项目(31301304,31101403);国家科技(转基因)重大专项(2014ZX0800904B);公益性行业科研专项(201203014);广东省自然科学基金资助项目(S2012010010018);国家重点实验室开放课题资助项目(SKLCUSAb201310);现代农业产业技术体系建设专项(CARS0124, 粤财教\[2009\]356 号)。

Abstract: Specific molecular markers for detecting the alleles of Pi2/Pi9/Pizt, designated as Pi2SNP, Pi9SNP and PiztPA, respectively, have been successfully developed through sequence comparison method. These three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus, which facilitate the molecular markerassistant selection and the development of pyramiding cultivars combined with other allele R genes. Additionally, 101 main cultivars and breeding parents collected from different riceplanting areas have been diagnosed with these three specific markers, and the results showed that only 2 out of 101 cultivars carried Pizt, but neither Pi2 nor Pi9 band had been detected. So far, it indicated that most of the main cultivars and breeding parents in China do not carry  Pi2/Pi9/Pizt. These results had provided important information for the application of Pi2/Pi9/Pizt in the blast resistance breeding programs.

Key words: rice, blast, resistance gene, molecular marker, MAS, Pi2/9 locus

摘要: 通过对已克隆的抗稻瘟病基因Pi2、Pi9以及Pizt 进行序列比对,寻找各自特异的核苷酸差异,成功开发了基于PCR技术以及电泳检测技术的 Pi2/Pi9/Pizt  以及Pizt 的基因特异性分子标记,能有效地将 Pi2/Pi9/Pizt  与该位点上的其他抗性等位基因及感病等位基因区分开,这为分子标记辅助育种及抗病基因聚合提供有效的分子标记。用 Pi2/Pi9/Pizt 基因特异性分子标记对来自全国各稻区的共101份水稻品种和育种亲本进行分子检测,结果发现,除2个品种检测到Pizt带型之外,大部分水稻品种不携带这3个抗性基因,这为有目的地开展品种的抗性改良提供了重要的参考信息。

关键词: 水稻, 稻瘟病, 抗性基因, 分子标记, 分子标记辅助选择育种, Pi2/9 位点

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