中国水稻科学

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SCAR 标记对水稻苯达松敏感致死基因的辅助选育

向太和1,2; 杨剑波2; 杨前进3; 朱启升3; 李莉2; 黄大年1,4   

  1. 1浙江大学 生命科学学院, 浙江 杭州 310012; 2安徽省农业科学院 水稻研究所, 农业部水稻遗传育种重点开放实验室, 安徽 合肥 230031;
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-04-10 发布日期:2003-04-10

SCAR Markers Assisted Selection for a Bentazon Susceptible Lethality Gene (ben) in Rice

XIANG Tai-he 1; 2; YANG Jian-bo 2; YANG Qian-jin 3; ZHU Qi-sheng 3; LI Li 2; HUANG Da-nian 1; 4   

  1. ( 1College of Life Sciences; Zhejiang University; Hangzhou 310012; China; 2 Rice Research Institute; Anhui Academy of Agricultural Sciences; Key Laboratory of Rice Genetics Breeding; Ministry of Agriculture; Hefei 230031; 3 Natural Nourishing Food Engineering Institute; 4China National Rice Research Institute; Hangzhou 310006, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-04-10 Published:2003-04-10

摘要: 利用与苯达松敏感致死基因(ben)紧密连锁的SCAR标记,对田间以农林8号m为ben基因供体转育的后代进行了PCR检测。利用该标记不仅能鉴别出转育的材料是否含有ben基因,而且能区别它是含ben基因的纯合体还是杂合体,从而有效地指导田间选育,加速育种进程。

关键词: 水稻, 苯达松敏感致死基因, 分子标记辅助选育, 育种

Abstract:

With rice cultivar Norin 8m susceptible to bentazon as donor of ben gene, SCARs tightly linked to ben were utilized for selection of ben. Results showed that homozygous and heterozygous genotypes with ben could be identified with the SCARs. The molecular markers offered a powerful tool for indirect selection of ben and could accelerate the introgression of ben into current rice cultivars.

Key words: rice, bentazon susceptible lethality gene, molecular marker assisted selection, breeding