中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻分支酶基因 Sbe1和 Sbe3 cDNA的克隆及全序列分析

陈秀花1; 刘巧泉1，2; 吴信淦1; 王宗阳3; 顾铭洪1   

  1. 1扬州大学 农学院， 江苏 扬州 225009； 2扬州大学 生物科学与技术学院， 江苏 扬州 225009； 3中国科学院 上海植物生理生态研究所， 上海 200032
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-04-10 发布日期:2003-04-10

cDNA Cloning and Sequence Analysis of Rice Sbe1 and Sbe3 Genes

CHEN Xiu-hua 1; LIU Qiao-quan 1; 2; WU Hsin-gang 1; WANG Zong-yang 3; GU Ming-hong 1   

  1. （ 1Agricultural College; Yangzhou University; Yangzhou 225009; China; 2College of Bioscience and Biotechnology; 3Shanghai Institute of Plant Physiology and Ecology; Chinese Academy of Sciences; Shanghai 200032; China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-04-10 Published:2003-04-10

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摘要： 通过改良的RTPCR法合成了水稻未成熟种子胚乳cDNA库，并以此为模板，用PCR技术从粳稻品种武运粳7号中克隆了分别编码淀粉分支酶SBEⅠ和SBEⅢ的2个基因Sbe1和Sbe3。序列分析表明，克隆Sbe1和Sbe3基因的大小分别为2490 bp和2481 bp，包含了基因完整的编码序列。Sbe3基因与已发表基因全序列完全相同，同源性达100%；Sbe1基因与已发表基因序列有4个碱基不同，同源性为99.84%，推导氨基酸序列的差异为2个。

关键词: 水稻, 淀粉分支酶基因, 基因克隆, cDNA序列, 序列分析

Abstract: The starch branching enzyme (SBE) is a key enzyme in amylopectin biosynthesis and there are two major isoforms in rice, named SBEⅠ and SBEⅢ, which are encoded by the Sbe1 and Sbe3 genes, respectively. These two genes were cloned from the template cDNA library, which was synthesized by improved RT PCR technique from the mRNAs of the immature rice seeds of japonica rice Wuyunjing 7. DNA sequencing analysis showed that the size of the cloned Sbe1 and Sbe3 cDNAs were 2490 bp and 2481 bp, respectively, and carried their entire coding sequences. Comparison analysis indicated that the Sbe3 sequence was the same as the reported and their homology was 100%. There were only four base pairs difference, which resulted in two deduced amino acids alteration, between the cloned Sbe1 cDNA and the reported.

Key words: Oryza sativa, starch branching enzyme gene, gene clone, cDNA sequence, sequence analysis