中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

褐飞虱对甲胺磷抗性发展与解毒酶系活性的关系

刘泽文; 韩召军; 张玲春   

  1. 南京农业大学 农业部病虫监测与治理基础实验室， 江苏 南京 210095
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-04-10 发布日期:2003-04-10

Relationship Between Methamidophos Resistance Development and Three Detoxifying Enzymes in Brown Planthopper

LIU Ze-wen; HAN Zhao-jun; ZHANG Ling-chun   

  1. （Key Laboratory of Monitoring and Management of Plant Diseases and Insects; Ministry of Agriculture; P.R.China; Nanjing Agricultural University; Nanjing 210095; China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-04-10 Published:2003-04-10

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被引次数

15

摘要： 室内抗性筛选表明，褐飞虱对甲胺磷抗性的发展呈不规则的“S”形：第5代前抗性上升很慢，第5~15代间迅速上升，第15代后上升变慢。酯酶在抗药性上升中可能起着十分重要作用，随着抗性的上升，酯酶和个体酶活分布都向高活性发展。敏感种群、田间种群及其筛选世代F[sub]5[/sub]、F[sub]10[/sub]和F[sub]15[/sub]的酯酶活性与抗性倍数具有显著的相关性，相关系数达到0.98993。多功能氧化酶和谷胱甘肽转移酶在抗性发展中也可能起到一定作用，但活性的上升都集中在第10代之前，第10代之后变化不大。

关键词: 褐飞虱, 甲胺磷, 抗药性, 解毒酶

Abstract: Methamidophos resistance of brown planthopper, Nilaparvata lugens (BPH) was selected in laboratory. Results showed that the resistance development of BPH was approximatively shaped as the letter “S”: resistance change was small before the fifth generation and after the fifteenth generation, and the change was sharp between the fifth and the fifteenth generation. Esterase might play an important role in the resistance development, because the esterase activity and the number of individuals with high activities increased along with the resistance development. The esterase activities of insecticide sensitive population, field population F0, and its selective generations F5, F10 and F15 were high correlated with the resistance ratios of these generations, and the coefficient was 0.98993. Mixed function oxidases and glutathione S transferase also might play some roles in the resistance development, but the big change in the activities of the two detoxifying enzymes both took place before the tenth generation.

Key words: Nilaparvata lugens, methamidophos, resistance to insecticide, detoxifying enzymes