中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

药用野生稻抗稻褐飞虱鉴定与利用技术研究

秦学毅1； 朱汝财1；韦素美2；武波3；黄凤宽2；李道远1；唐建淮1   

  1. 1广西农业科学院 水稻研究所， 广西 南宁 530007； 2广西农业科学院 植物保护研究所， 广西 南宁 530007； 3广西大学 生命科学与技术学院， 广西 南宁 530004
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-11-10 发布日期:2004-11-10

Technique for Utilization and Identification of Resistance to Brown Planthopper in Oryza officinalis

QIN Xue-yi1; ZHU Ru-cai1; WEI Su-mei2; WU Bo3; HUANG Feng-kuan2; LI Dao-yuan1; TANG Jian-huai1   

  1. 1Institute of Crop Genetic Resources; Guangxi Academy of Agricultural Sciences; Nanning 530007; China; 2Institute of Plant Protection; Guangxi Academy o f Agricultural Sciences; 3 College of Life Science & Technology; Nanning 530004; China
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-11-10 Published:2004-11-10

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被引次数

25

摘要： 从198份药用野生稻资源中筛选出一批广谱高抗褐飞虱抗源（其中3份为免疫级）并对这些抗源进行了抗性遗传研究。研究结果表明其抗性是受一对显性基因控制的。通过离体幼胚培养获得绿苗，经过多代回交和自交，成功地将抗性基因转移到栽培稻中，获得了高世代（B4F5)株系。同时利用外源DNA花粉管导入法对药用野生稻广谱高抗褐飞虱基因导入到栽培稻进行了研究，获得了具有某些性状的后代。

关键词: 药用野生稻, 抗虫性, 褐飞虱, 遗传, 远缘杂交

Abstract: Three immune and 89 highly resistant wild rice ma te rials to brown planthopper were selected from 198 tested collections of O.of ficinalis. Genetic analysis indicated that the inheritance to brown plantho pper was conditioned by a pair of dominant gene. When Oryza officinalis with high resistance to brown planthopper was crossed with O. sativa by routine breeding and biotechnique with different hybridization ways and varieties, F1 hybrids could be achieved by embryo rescue tec hnique, and the resistance gene of O. officinalis was introgres sed into O. sativa by backcrossing and inbred. Exogenous DNA of the resistance of O. officinalis could be also introduced into O. sativa by the technique of pollen tube passage way, and some offsprings were obtained.

Key words: Oryza officinalis, resistance to insect pest, brown planthopper, inheritance, distant hybridization