中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

北方杂交粳稻骨干亲本遗传差异的SSR标记检测

邱福林; 庄杰云; 华泽田; 王彦荣; 程式华   

  1. 1辽宁省稻作研究所, 辽宁 沈阳 110101; 2中国水稻研究所, 浙江 杭州 310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-03-10 发布日期:2005-03-10

Inspect of Genetic Differentiation of Main Parents of japonica Hybrid Rice in the Northern China by Simple Sequence Repeats(SSR)

QIU Fu-lin , ZHUANG Jie-yun , HUA Ze-tian , WANG Yan-rong , CHENG Shi-hua   

  • Received:1900-01-01 Revised:1900-01-01 Online:2005-03-10 Published:2005-03-10

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摘要： 利用微卫星（SSR)分子标记对北方杂交粳稻骨干亲本进行了遗传差异鉴定和籼性程度分析。以15对SSR引物对模板DNA扩增产生52条多态性标记，每个引物平均提供3.47个标记信息。用平均距离法（UPMGA）进行聚类分析，骨干亲本在相似系数0.69处可分成5组，各组间亲本的遗传差异相对较大；23个骨干亲本的籼性程度参数值的变化幅度为0.12～0.48。

关键词: 杂交水稻, 骨干亲本, 分子标记, 遗传差异, 籼性程度

Abstract: Genetic differentiation and indica component degree analysis were conducted on the main parents of japonica hybrid rice in the northern China by simple sequence repeats(SSR). The genomic DNAs of the main parents were amplified with 15 primers. Fifty two polymorphic markers were generated in all. Every primer offered 3.47 markers on average. The molecular phylogenetic tree by UPMGA method showed that main parents were divided into five groups at genetic similarity coefficient 0 69. The parents between groups have great genetic differentiation relatively. The extent of indica component value was from 0 12 to 0.48 in the parents.

Key words: hybrid rice, main parents, molecular marker, genetic differentiation, indica component degree