中国水稻科学 ›› 2020, Vol. 34 ›› Issue (2): 95-103.DOI: 10.16819/j.1001-7216.2020.9130
• 综述与专论 • 下一篇
收稿日期:
2019-12-05
修回日期:
2019-12-18
出版日期:
2020-03-10
发布日期:
2020-03-10
通讯作者:
唐文帮
基金资助:
Wenbang TANG*(), Guilian ZHANG, Huabing DENG
Received:
2019-12-05
Revised:
2019-12-18
Online:
2020-03-10
Published:
2020-03-10
Contact:
Wenbang TANG
摘要:
居高不下的杂交水稻制种成本已经成为制约杂交稻推广应用的主要因素之一。本文详细地分析了国内外杂交水稻机械化制种的现状,总结机械化制种存在的困难和问题,针对我国当前形势和耕作制度的特点,结合多年育种实践,提出了通过培育小粒型不育系,利用不育系与恢复系种子粒型(主要是粒厚)的显著差异,实现父母本混播混收、收获后机械分离获得杂交种子的全程机械化制种设想。提出了适合机械化制种的小粒不育系应具备的性状特征。按此设想,笔者团队选育出了综合性状优良的小粒型不育系卓201S、南3502S、展998S等,并配组了系列高产、优质的杂交稻组合卓两优581、卓两优141、南两优1998等,成功实现这些杂交稻的高效机械化制种。卓201S等小粒型不育系及其系列杂交组合的育成,是杂交水稻机械化制种品种选育的重大突破。
中图分类号:
唐文帮, 张桂莲, 邓化冰. 杂交水稻机械化制种的技术探索与实践[J]. 中国水稻科学, 2020, 34(2): 95-103.
Wenbang TANG, Guilian ZHANG, Huabing DENG. Technology Exploration and Practice of Hybrid Rice Mechanized Seed Production[J]. Chinese Journal OF Rice Science, 2020, 34(2): 95-103.
图1 卓201S的株型及籽粒性状 A-卓201S的株型; B-卓201S与恢复系R2115粒型比较, 红色箭头所示为R2115种子, 卓201S千粒重14.10 g, 粒厚1.71 mm, R2115千粒重33.40 g, 粒厚2.21 mm; C-卓201S种子外观品质。
Fig. 1. Plant type and grain characters of Zhuo 201S. A, Plant type of Zhuo 201S; B, Comparison of grain types between Zhuo 201S and the restorer line R2115, the grains with red arrow shows R2115 seed, the 1000 grains weight of Zhuo 201S is 14.10 g, the grain thickness of Zhuo 201S is 1.71 mm, the 1000-grain weight of R2115 is 33.40 g, the grain thickness of Zhuo 201S is 2.21 mm; C, The seed appearance quality of Zhuo 201S.
不育系 SL | 株高 PH /cm | 粒长 GL /cm | 粒宽 GW /mm | 粒厚 GT /mm | 千粒重 GW /g | 稻米品质 GQ | 育性转换温度 FTT/℃ | 柱头 外露率 SER/% | 柱头活力 SV /% | 穗包颈率 PER/% | 赤霉酸用量 GA/(g∙hm-2) | 代表组合 Representative combination | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
卓201S Zhuo 201S | 60.0 | 0.62 | 1.84 | 1.71 | 14.1 | 优良Excellent | <23.0 | 91.7 | 34.1 | 5.1 | 225 | 卓两优141 Zhuoliangyou 141 | |
南3502S Nan 3502S | 62.1 | 0.59 | 1.92 | 1.78 | 19.2 | 优良Excellent | <23.0 | 93.2 | 30.1 | 6.4 | 270 | 南两优1998 Nanliangyou 1998 | |
展998S Zhan 998S | 60.6 | 0.60 | 1.80 | 1.71 | 14.0 | 优良Excellent | <23.0 | 86.3 | 20.1 | 1.3 | 225 | 展两优018 Zhanliangyou 018 | |
C815S(CK) | 68.3 | 0.66 | 1.97 | 2.10 | 24.3 | 一般Common | <23.0 | 72.8 | 1.7 | 16.9 | 375 | C两优华占 C liangyouhuazhan |
表1 小粒型不育系具有适合机械化制种的性状
Table 1 Small grain male sterile lines suitable for mechanized seeds production.
不育系 SL | 株高 PH /cm | 粒长 GL /cm | 粒宽 GW /mm | 粒厚 GT /mm | 千粒重 GW /g | 稻米品质 GQ | 育性转换温度 FTT/℃ | 柱头 外露率 SER/% | 柱头活力 SV /% | 穗包颈率 PER/% | 赤霉酸用量 GA/(g∙hm-2) | 代表组合 Representative combination | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
卓201S Zhuo 201S | 60.0 | 0.62 | 1.84 | 1.71 | 14.1 | 优良Excellent | <23.0 | 91.7 | 34.1 | 5.1 | 225 | 卓两优141 Zhuoliangyou 141 | |
南3502S Nan 3502S | 62.1 | 0.59 | 1.92 | 1.78 | 19.2 | 优良Excellent | <23.0 | 93.2 | 30.1 | 6.4 | 270 | 南两优1998 Nanliangyou 1998 | |
展998S Zhan 998S | 60.6 | 0.60 | 1.80 | 1.71 | 14.0 | 优良Excellent | <23.0 | 86.3 | 20.1 | 1.3 | 225 | 展两优018 Zhanliangyou 018 | |
C815S(CK) | 68.3 | 0.66 | 1.97 | 2.10 | 24.3 | 一般Common | <23.0 | 72.8 | 1.7 | 16.9 | 375 | C两优华占 C liangyouhuazhan |
机械化制种方式 MSPM | 始穗期差 DHD/d | 代表组合 Representative combination | 父本播种方式 PSM | 母本播种方式 FSM | 父母本比例 M∶F | 父母本花期 FP | 赤霉酸用量 GA/(g∙hm-2) | 辅助授粉 AP | 制种产量 YSP /(kg∙hm-2) | |
---|---|---|---|---|---|---|---|---|---|---|
父母本混直播 Parent-mixed direct seeding | 7 | 卓两优780 Zhuoliangyou 780 | 播芽谷 Sowing bud seed | 播干谷 Sowing dry seed | 1∶28 | 相遇 Meet | 225 | 不需要 No | 5050.5 | |
父抛秧、母直播 Male broadcast- transplanting, female parent direct seeding | 12 | 卓两优581 Zhuoliangyou 581 | 提前20 d 软盘育秧 Raising seedlings | 播芽谷 Sowing bud seed | 父本8250穴/hm2 Male parent 8250 plants/hm2 | 相遇 Meet | 225 | 不需要 No | 5283.0 | |
父母本同条播 Parents sowing in line | 0 | 卓两优1998 Zhuoliangyou 1998 | 条播干谷 Sowing dry seed in line | 条播芽谷 Sowing bud seed in line | 1∶9 | 相遇 Meet | 225 | 需要 Need | 4446.0 |
表2 基于小粒型不育系的三种机械化制种方式
Table 2 Three mechanized seed production methods based on the small grain male sterile lines.
机械化制种方式 MSPM | 始穗期差 DHD/d | 代表组合 Representative combination | 父本播种方式 PSM | 母本播种方式 FSM | 父母本比例 M∶F | 父母本花期 FP | 赤霉酸用量 GA/(g∙hm-2) | 辅助授粉 AP | 制种产量 YSP /(kg∙hm-2) | |
---|---|---|---|---|---|---|---|---|---|---|
父母本混直播 Parent-mixed direct seeding | 7 | 卓两优780 Zhuoliangyou 780 | 播芽谷 Sowing bud seed | 播干谷 Sowing dry seed | 1∶28 | 相遇 Meet | 225 | 不需要 No | 5050.5 | |
父抛秧、母直播 Male broadcast- transplanting, female parent direct seeding | 12 | 卓两优581 Zhuoliangyou 581 | 提前20 d 软盘育秧 Raising seedlings | 播芽谷 Sowing bud seed | 父本8250穴/hm2 Male parent 8250 plants/hm2 | 相遇 Meet | 225 | 不需要 No | 5283.0 | |
父母本同条播 Parents sowing in line | 0 | 卓两优1998 Zhuoliangyou 1998 | 条播干谷 Sowing dry seed in line | 条播芽谷 Sowing bud seed in line | 1∶9 | 相遇 Meet | 225 | 需要 Need | 4446.0 |
杂交组合 HC | 父本粒厚 PGT/mm | 杂交种粒厚 HSGT/mm | 第一级筛 FLS/mm | 第二级筛 SLS/mm | 入机混收种 MH/kg | 选出杂交种 SHS/kg | 选出父本 SMS/kg | 分选成功率 SR/% | 损失率 LR/% | 效率 E/(t∙h-1) |
---|---|---|---|---|---|---|---|---|---|---|
卓两优581 Zhuoliangyou 581 | 2.05 | 1.71 | 2.0 | 2.1 | 159.7 | 108.10 | 51.68 | 100.0 | 1.31 | 2.0 |
卓两优2115 Zhouliangyou 2115 | 2.21 | 1.71 | 2.1 | 2.1 | 118.7 | 88.17 | 30.60 | 100.0 | 0.51 | 2.5 |
卓两优141 Zhouliangyou 141 | 1.97 | 1.71 | 2.0 | 2.1 | 137.7 | 98.71 | 39.01 | 98.4 | 2.31 | 2.0 |
表3 父本及杂交种机械分选结果
Table 3 The results of mechanical sorting of parents and combinations.
杂交组合 HC | 父本粒厚 PGT/mm | 杂交种粒厚 HSGT/mm | 第一级筛 FLS/mm | 第二级筛 SLS/mm | 入机混收种 MH/kg | 选出杂交种 SHS/kg | 选出父本 SMS/kg | 分选成功率 SR/% | 损失率 LR/% | 效率 E/(t∙h-1) |
---|---|---|---|---|---|---|---|---|---|---|
卓两优581 Zhuoliangyou 581 | 2.05 | 1.71 | 2.0 | 2.1 | 159.7 | 108.10 | 51.68 | 100.0 | 1.31 | 2.0 |
卓两优2115 Zhouliangyou 2115 | 2.21 | 1.71 | 2.1 | 2.1 | 118.7 | 88.17 | 30.60 | 100.0 | 0.51 | 2.5 |
卓两优141 Zhouliangyou 141 | 1.97 | 1.71 | 2.0 | 2.1 | 137.7 | 98.71 | 39.01 | 98.4 | 2.31 | 2.0 |
图3 杂交稻卓两优780混播混收机械化制种及种子分选 A-卓两优780混播制种苗期田间照片;B-卓两优780混播制种分蘖期田间照片;C-卓两优780混播制种成熟期田间照片;D-混收后利用机器分选杂交种与恢复系R780种子;E-分选机器所用的狭长形筛孔筛子(20 mm×2 mm);F-分选后的卓两优780杂交种(左)和恢复系R780(右)种子;图A, 图B, 图C中红色箭头所示为R780植株。
Fig. 3. Mechanized seed production and seed sorting of two line hybrid rice Zhuoliangyou 780. A, Field picture of seedling stage of hybrid seed production for Zhuoliangyou780 with mixed and direct seeding way. B, Field picture at booting stage of hybrid seed production for Zhuoliangyou780 with mixed and direct seeding way. C, Field picture at maturation stage of hybrid seed production for Zhuoliangyou780 with mixed and direct seeding way. D, Sorting the seeds between hybrid rice and restorer line R780 by machine. E, The long and narrow mesh sieve used in sorting machine. F, Seeds of hybrid rice Zhuoliangyou 780 (left) and restorer line R780 (right) after sorting. The red arrow shows R780 plant.
制种方式 SPM | 种植方式 PM | 虫害防治 PCM | 辅助授粉APM | 收割方式 HM | 母本容量 MC | 平均异交率 AOR/% | 平均产量 AY/(kg∙hm-2) | 种子质量 SQ | 制种综合效益 CBOSP/(Yuan∙hm-2) |
---|---|---|---|---|---|---|---|---|---|
C815S传统制种 Traditional seed production of C815S | 移栽 Transplanting | 人工 Manual | 人工 Manual | 父母本分开收 Parents separate harvesting | - | 60.4 | 2625 | 无休眠、黑粉病重 No dormancy, no smut disease | 52 500 |
卓201S机械化制种 Mechanized seed production of Zhuo 201S | 父母本机械混合直播Mechanical mixed and direct seeding | 飞机 Airplane | 飞机 Airplane | 机械混收 Mechanical harvesting | 增加20% Increased by 20% | 70.3 | 3150 | 有休眠、无开颖、无黑粉病 Dormancy, no glume opening, no smut disease | 63 000 +8 550(减少投入) |
增加效率 Increase efficiency | 4500 Yuan/hm2 | 750 Yuan/hm2 | 1800 Yuan/hm2 | 1500 Yuan/hm2 | 525 kg/hm2 (+20%) | - | 19 050(+36.3%) |
表4 卓201S机械化制种与C815S传统制种效益比较
Table 4 Comparison of benefit between Zhuo 201S mechanized seed production and C815S traditional seed production.
制种方式 SPM | 种植方式 PM | 虫害防治 PCM | 辅助授粉APM | 收割方式 HM | 母本容量 MC | 平均异交率 AOR/% | 平均产量 AY/(kg∙hm-2) | 种子质量 SQ | 制种综合效益 CBOSP/(Yuan∙hm-2) |
---|---|---|---|---|---|---|---|---|---|
C815S传统制种 Traditional seed production of C815S | 移栽 Transplanting | 人工 Manual | 人工 Manual | 父母本分开收 Parents separate harvesting | - | 60.4 | 2625 | 无休眠、黑粉病重 No dormancy, no smut disease | 52 500 |
卓201S机械化制种 Mechanized seed production of Zhuo 201S | 父母本机械混合直播Mechanical mixed and direct seeding | 飞机 Airplane | 飞机 Airplane | 机械混收 Mechanical harvesting | 增加20% Increased by 20% | 70.3 | 3150 | 有休眠、无开颖、无黑粉病 Dormancy, no glume opening, no smut disease | 63 000 +8 550(减少投入) |
增加效率 Increase efficiency | 4500 Yuan/hm2 | 750 Yuan/hm2 | 1800 Yuan/hm2 | 1500 Yuan/hm2 | 525 kg/hm2 (+20%) | - | 19 050(+36.3%) |
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