中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

利用显性选择标记基因转化稻瘟病菌（英文）

陶全洲   

  1. 中国科学院上海生物化学研究所，上海200031
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1993-10-10 发布日期:1993-10-10

Transformation of Pyricularia oryzae with a Dominant Selectable Marker (in English)

Tao Quanzhou   

  • Received:1900-01-01 Revised:1900-01-01 Online:1993-10-10 Published:1993-10-10

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摘要： 利用抗药性标记基因(Hygromycin B, hygB)建立稻瘟病菌的转化系统。结果表明， Aspergillus的TrpC转录信号与细菌的hygB抗性结构基因重组的质粒pCSN43可取在稻瘟病菌中表达，该质粒与受体菌无同源顺序，因而有利于对转化菌株进行筛选和分析。 电激法和PEG/Ca2＋ 法均适用于转化稻瘟病菌，但后者的转化效率比前者高。

关键词: 显性标记基因, 稻瘟病, 转化, 水稻

Abstract: A transformation system based on a dominant selectable marker, hygromycin B resistance (hyg B′) gene, was established for Pyricularia oryzae. The plasmid pCSN43 conlaining a hyg B′ structure gene with a Trp C transcription signal of Aspergillus was functional in P. oryzae. There are neither homologous sequences of the marker in the recipient genome nor any hyg B′ spontaneous mutants. The transformation system offerred a clean background and facilitated selection and identification of transformants. Both electroporation and PEG/Ca2+ methods worked for transformation, and the high est transformation frequency was 25 transformants per microgram vector DNA on PEG/Ca2+ method.

Key words: Dominant selectable marker, Hygromycin B resistance gene, Pyricularia oryzae, Transformation