中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

麦芽糖提高水稻花药培养效率的研究

孙宗修；斯华敏；程式华；湛小燕   

  1. 中国水稻研究所，杭州310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1993-10-10 发布日期:1993-10-10

Effect of Maltose on Efficiency of Anther Culture of Rice

Sun Zongxiu; Si Huamin; Zhan Xiaoyan   

  • Received:1900-01-01 Revised:1900-01-01 Online:1993-10-10 Published:1993-10-10

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被引次数

93

摘要： 研究了浓度均为150 mmol/L的9种碳源(含组合)对3个籼稻品种花药的愈伤组织诱导率的影响，发现麦芽糖为碳源的培养基效果最佳。进一步以7个籼稻2个粳稻和4个杂交组合为材料，系统比较了150 mmol/L的麦芽糖与蔗糖作为诱导培养基的碳源对花药培养的影响，结果表明，以麦芽糖为碳源的培养基上，平均愈伤组织诱导率和随后的绿苗分化率分别为54.81%与9.70%，而在相应的蔗糖培养基上只有11.85%和3.30%，差异极显著。讨论了提高籼稻花培效率的簧略，井建议采用统一的方法计算愈伤组织诱导率．

关键词: 花药培养, 麦芽糖, 水稻, 培养基

Abstract: The effect of different sugars (150 mmol/L for each) on callus induction in rice anther culture was initially investigated by using three indica cultivars. Maltose resulted in greater microspore callus induction than sucrose and other sugars. Further experiments with 7 indica, 2 japonica cultivars and several hybrids indicated that mean percentages of callus induction and then green plantlet regeneration were 54. 8% and 9. 70%, respectively, on maltose medium, which were significantly higher than those on sucrose medium. The strategy of raising the efficiency of indica rice anther culture and suitable calculative formula for callus induction rate are discussed.

Key words: Anther culture, Callus induction, Green plant regeneration.sugar, Oryza sativa