中国水稻科学 ›› 2021, Vol. 35 ›› Issue (2): 200-206.DOI: 10.16819/j.1001-7216.2021.0815
• 研究报告 • 上一篇
韦雪露#, 卢柏亦#, 黄福钢, 李发活, 刘芳, 邱永福*()
收稿日期:
2020-08-22
修回日期:
2020-09-16
出版日期:
2021-03-10
发布日期:
2021-03-10
通讯作者:
邱永福
作者简介:
#共同第一作者
基金资助:
Xuelu WEI#, Baiyi LU#, Fugang HUANG, Fahuo LI, Fang LIU, Yongfu QIU*()
Received:
2020-08-22
Revised:
2020-09-16
Online:
2021-03-10
Published:
2021-03-10
Contact:
Yongfu QIU
About author:
#These authors contributed equally to this work
摘要:
【目的】研究白背飞虱取食后苗期水稻内源激素含量变化规律及其合成途经相关基因的差异表达,为进一步解析内源激素调控水稻白背飞虱抗性机理提供参考。【方法】利用UPLC-MS方法测定了白背飞虱敏感材料9311与抗性近等基因系(NIL)在白背飞虱取食0、24和48 h后植株水杨酸(SA)、脱落酸(ABA)、茉莉酸(JA)和生长素(IAA)4种激素含量的变化;qRT-PCR分析5个与激素合成途径相关基因在抗、感植株中表达水平的差异。【结果】激素含量测定结果表明,白背飞虱取食的48 h内,NIL中SA含量先升后降,而在9311中先降后升;ABA的含量在NIL中波动较小,而在9311中先升后降;IAA和JA在抗、感植株中均持续上升。激素合成途径相关基因OsPAL06、OsZEP、OsLOX和OsYUCCA1在接虫后24 h的表达量差异在抗、感植株中均达到显著或极显著水平,而在0 h和48 h差异不显著。OsICS1则在接虫后48 h的表达量差异在抗、感植株达到显著水平,而接虫0和24 h后差异不显著。【结论】在接虫或者对照处理中,JA和ABA含量在抗性植株中均高于感虫植株,可能与抗性基因有较大关系;而SA含量和OsPAL06、OsICS1基因表达水平均表明抗性植株对白背飞虱的取食响应更迅速,在抗虫过程中能够起到明显的调控作用。
韦雪露, 卢柏亦, 黄福钢, 李发活, 刘芳, 邱永福. 白背飞虱取食后水稻幼苗内源激素变化研究[J]. 中国水稻科学, 2021, 35(2): 200-206.
Xuelu WEI, Baiyi LU, Fugang HUANG, Fahuo LI, Fang LIU, Yongfu QIU. Changes in Endogenous Hormones in Rice Seedlings Infested by White-backed Planthoppers[J]. Chinese Journal OF Rice Science, 2021, 35(2): 200-206.
基因 Gene | 基因登录号 Gene ID | 序列(5'-3') Sequence (5'-3') | 相关激素 Associated hormone |
---|---|---|---|
OsPAL06 | Os02g41680 | F-GGGCAACCCAGTGACCAA R-CGATTGCCTCGTCGGTCTT | 水杨酸SA |
OsICS1 | Os09g19734 | F-TATGGTGCTATCCGCTTCGAT R-CGAGAACCGAGCTCTCTTCAA | 水杨酸SA |
OsZEP | Os04g37619 | F-GGATGCCATTGAGTTTGGTT R-TGGCTGACTGAAGTCTCG | 脱落酸ABA |
OsYUCCA1 | Os01g45760 | F-A TAATCCTCGCCACAGGCTACA R-TGGCTGACTGAAGTCTCTCG | 生长素IAA |
OsLOX | Os01g45760 | F-TATGGTGCTATCCGCTTCGAT R-CGAGAACCGAGCTCTCTTCAA | 茉莉酸JA |
Actin | Os11g06390 | F-GAGTATGATGAGTCGGGTCCAG R-ACACCAACAATCCCAAACAGAG | - |
表1 本研究所用引物序列
Table 1 Primers used in the study.
基因 Gene | 基因登录号 Gene ID | 序列(5'-3') Sequence (5'-3') | 相关激素 Associated hormone |
---|---|---|---|
OsPAL06 | Os02g41680 | F-GGGCAACCCAGTGACCAA R-CGATTGCCTCGTCGGTCTT | 水杨酸SA |
OsICS1 | Os09g19734 | F-TATGGTGCTATCCGCTTCGAT R-CGAGAACCGAGCTCTCTTCAA | 水杨酸SA |
OsZEP | Os04g37619 | F-GGATGCCATTGAGTTTGGTT R-TGGCTGACTGAAGTCTCG | 脱落酸ABA |
OsYUCCA1 | Os01g45760 | F-A TAATCCTCGCCACAGGCTACA R-TGGCTGACTGAAGTCTCTCG | 生长素IAA |
OsLOX | Os01g45760 | F-TATGGTGCTATCCGCTTCGAT R-CGAGAACCGAGCTCTCTTCAA | 茉莉酸JA |
Actin | Os11g06390 | F-GAGTATGATGAGTCGGGTCCAG R-ACACCAACAATCCCAAACAGAG | - |
图1 苗期抗白背飞虱鉴定结果 A–苗期抗虫鉴定;B–抗性值。**表示差异极显著(P < 0.01)
Fig. 1. WBPH resistance identification result at seedling stage. A, Insect resistance test at seedling stage; B, Resistance score; ** indicates significant difference (P < 0.01).。
图3 白背飞虱取食后SA和JA合成途径相关基因在抗、感材料中的表达量分析*和**表示在抗、感材料中表达量存在显著和极显著差异。
Fig. 3. Relative expression levels of SA and JA metabolic pathway associated genes between resistant and susceptible lines after WBPH infestation. *and ** indicate significant difference at P < 0.05 and P < 0.01 levels, respectively.
图4 白背飞虱取食后ABA和IAA合成途径相关基因在抗、感材料中的表达量分析*和**表示在抗、感材料中表达量存在显著和极显著差异。
Fig. 4. Relative expression level of ABA and IAA metabolic pathway associated genes between resistant and susceptible lines after WBPH infestation. *and ** indicate significant difference at P < 0.05 and P < 0.01 levels, respectively.
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