中国水稻科学 ›› 2018, Vol. 32 ›› Issue (5): 453-461.DOI: 10.16819/j.1001-7216.2018.7135

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Pigm特异性选择标记的开发及其在粳稻穗颈瘟抗性育种中的利用

曾生元, 李闯, 杜灿灿, 孙立亭, 景德道, 林添资, 余波, 钱华飞, 姚维成, 周义文, 龚红兵*()   

  1. 江苏丘陵地区镇江农业科学研究所,江苏 句容 212400;
  • 收稿日期:2017-11-09 修回日期:2018-01-30 出版日期:2018-09-10 发布日期:2018-09-10
  • 通讯作者: 龚红兵
  • 基金资助:
    国家重点研发计划资助项目(2017YFD0100400);镇江市科技支撑计划资助项目(NY2015018);江苏省重点研发计划资助项目(BE2015363);江苏省农业科技自主创新资金资助项目[CX(16)1029]

Development of Specific Markers for Pigm in Marker-Assisted Breeding of Panicle Blast Resistant japonica Rice

Shengyuan ZENG, Chuang LI, Cancan DU, Liting SUN, Dedao JING, Tianzi LIN, Bo YU, Huafei QIAN, Weicheng YAO, Yiwen ZHOU, Hongbing GONG*()   

  1. Zhenjiang Institute of Agricultural Sciences of the Ning-Zhen Hilly District, Jurong 212400, China;
  • Received:2017-11-09 Revised:2018-01-30 Online:2018-09-10 Published:2018-09-10
  • Contact: Hongbing GONG

摘要:

【目的】Pigm是一个广谱的稻瘟病抗性基因,源自持久抗性品种谷梅4号,与PizPiz-tPi2Pi9Pi40等互为复等位基因但抗谱存在差异。为了更好地在分子辅助选择育种中利用Pigm基因,开发与Pigm特异性标记具有重要意义。【方法】在已有文献报道定位结果的基础上,通过随机测序获得了一段谷梅4号基因组的特异序列,并据此开发了一组用于筛选Pigm基因的分子标记,进一步选取江淮稻区3个不同生态型代表性粳稻品种作为受体,利用分子标记辅助选择结合对抗性基因的背景检测将Pigm基因导入受体品种。【结果】Pigm-4标记位于Pigm基因簇内部,与抗病功能元件PigmR紧密连锁,对不同类型的品种检测发现该标记特异性强,且利用该标记可将PigmPizPiz-tPi2Pi9以及Pi40区分开来。对受体亲本稻瘟病抗性基因的检测和接种结果分析发现江淮稻区粳稻品种虽然携带了PibPi54PitaPb1中的2~3个基因,但是对强毒力的稻瘟病小种抗性普遍不强,而3种代表性粳稻背景下导入Pigm基因均可显著提高其对穗颈瘟的抗性水平。【结论】Pigm可以作为抗稻瘟病粳稻育种的有利基因资源加以利用,而Pigm-4是分子标记辅助筛选Pigm的优异标记。

关键词: Pigm, 特异分子标记, 粳稻, 穗颈瘟, 抗性

Abstract:

【Objective】 Pigm is a broad-spectrum resistance gene to rice blast, which is isolated from Gumei 4, a variety with durable resistance. Pigm is allelic to Piz, Piz-t, Pi2, Pi9, Pi40 and so on, but shows different resistance spectrum. To breed resistance variety carrying Pigm with molecular marker-assisted selection, discovering Pigm-tightly-linked markers is important.【Method】We identified a unique DNA sequence of Gumei 4 by genomic sequencing, and developed a set of specific markers tagged to Pigm base on the sequence divergence. Then, by means of molecular-assisted selection and background gene screening, Pigm was pyramided to three representative japonica varieties belonging to different ecotypes from Yangtze-Huai River Area. 【Result】 An InDel marker Pigm-4 which is closely linked to the functional unit of Pigm was developed. Pigm-4 showed distinct specificity by genotype screening of various rice varieties, and can distinguish the genotype of Pigm from its alleles Piz, Piz-t, Pi2, Pi9 and Pi40. Although receptors carry with resistant genes such as Pib+Pi54 (Wuyun 2674), Pib+Pita (Wuyunjing 27), Pib+Pi54+Pb1 (Zhendao 18), respectively, but none of them can defense high virulence blast pathogen. Relevant introgression lines were established and inoculations of introgression lines indicate that Pigm can promote resistance level to rice panicle blast significnatly in different japonica backgrounds. 【Conclusion】Pigm can be used as a beneficial gene source in the breeding of blast resistant japonica rice, and Pigm-4 is an excellent marker for the molecular marker-assisted selection of Pigm.

Key words: Pigm, specific-marker, japonica rice, panicle blast, resistance

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