中国水稻科学 ›› 2015, Vol. 29 ›› Issue (5): 546-558.DOI: 10.3969/j.issn.1001G7216.2015.05.012
• • 上一篇
收稿日期:
2015-01-08
修回日期:
2015-04-12
出版日期:
2015-09-10
发布日期:
2015-09-10
通讯作者:
郭龙彪,胡兴明
作者简介:
*通讯录作者:E-mail:guolongb@mail.hz.zj.cnhuxingmingx@126.com
基金资助:
Yong-tao CUI, Li-wen WU, Long-biao GUO*(), Xing-ming HU*()
Received:
2015-01-08
Revised:
2015-04-12
Online:
2015-09-10
Published:
2015-09-10
Contact:
Long-biao GUO, Xing-ming HU
About author:
*Corresponding author:E-mailguolongb@mail.hz.zj.cnhuxingmingx@126.com
摘要:
由α、β和γ 3个亚基组成的异源三聚体G蛋白是真核生物中一类重要的信号传导分子,在生长发育中起重要的调控作用。G蛋白通过细胞表面的偶联受体感知细胞外刺激,并由G 蛋白传送信号到胞内蛋白来影响细胞行为。植物与动物G蛋白虽然具有类似的分子结构,但植物信号的传递由非典型“自我激活”机制和效应蛋白来完成并控制植物生长发育。综述了植物G蛋白的组成;重点介绍了水稻G蛋白亚基编码基因 D1、 GS3、 DEP1和qNGR9参与激素、抗病、抗逆信号传导及水稻株型控制,粒型和N元素高效吸收利用的研究进展。另外,对G蛋白的偶联信号途径组分和功能、系统生物学和应用方面进行了展望。
中图分类号:
崔永涛, 吴立文, 郭龙彪, 胡兴明. 水稻异源三聚体G蛋白生理功能的研究进展[J]. 中国水稻科学, 2015, 29(5): 546-558.
Yong-tao CUI, Li-wen WU, Long-biao GUO, Xing-ming HU. Research Progress in Physiologic Functions of Heterotrimeric G Protein in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(5): 546-558.
种类/突变体 Type/Mutant | 数量 Number | 表型 Phenotype | 调控机制 Regulation mechanism | 生物学功能 Bio-function |
---|---|---|---|---|
Gα (rga1, d1) | 1 | 茎秆变短增粗;穗直立,着粒密,谷粒小而圆;第二节间不伸长 | 编码Gα 亚基 | 参与赤霉素信号途径与油菜素内酯(BR)的信号传导;介导抗稻瘟病反应;介导乙烯信号参与抗逆 |
Gβ (rgb1/RNAi) | 1 | 矮秆,穗和种子变小,叶角变小,节点处发生褐化 | 编码Gβ亚基,正调控细胞数量而不是细胞大小 | 控制细胞分化,当完全缺失时,植株死亡;参与抗逆性 |
Gγ (gs3) | 5 | 种子变大 | 编码Gγ,负调控籽粒和器官大小 | 控制纵向细胞数,决定谷粒大小 |
Gγ(dep1, qNGR9) | 稻穗变短、直立,着粒密集,每穗籽粒数增多 | 编码Gγ,促进细胞分裂,降低穗颈节长;稻穗变密,枝梗数增加,粒数增多 | 穗粒数增加,增加产量;介导N元素的吸收;可能介导抗逆反应 |
表1 水稻G蛋白3个亚基的功能
Table 1 Functions of three G protein subunits in rice.
种类/突变体 Type/Mutant | 数量 Number | 表型 Phenotype | 调控机制 Regulation mechanism | 生物学功能 Bio-function |
---|---|---|---|---|
Gα (rga1, d1) | 1 | 茎秆变短增粗;穗直立,着粒密,谷粒小而圆;第二节间不伸长 | 编码Gα 亚基 | 参与赤霉素信号途径与油菜素内酯(BR)的信号传导;介导抗稻瘟病反应;介导乙烯信号参与抗逆 |
Gβ (rgb1/RNAi) | 1 | 矮秆,穗和种子变小,叶角变小,节点处发生褐化 | 编码Gβ亚基,正调控细胞数量而不是细胞大小 | 控制细胞分化,当完全缺失时,植株死亡;参与抗逆性 |
Gγ (gs3) | 5 | 种子变大 | 编码Gγ,负调控籽粒和器官大小 | 控制纵向细胞数,决定谷粒大小 |
Gγ(dep1, qNGR9) | 稻穗变短、直立,着粒密集,每穗籽粒数增多 | 编码Gγ,促进细胞分裂,降低穗颈节长;稻穗变密,枝梗数增加,粒数增多 | 穗粒数增加,增加产量;介导N元素的吸收;可能介导抗逆反应 |
图4 GS3和DEP1的结构特点及其作用方式 (引自 Botella[58], 略有修改)
Fig. 4. Structural characteristics and action pattern of GS3 and DEP1(From Botella[58], with some nodifications).
[1] | Gilman A G.G proteins: Transducers of receptor-generated signals.Annu Rev Biochem, 1987, 56: 615-649. |
[2] | Wettschureck N, Offermanns S.Mammalian G proteins and their cell type specific functions.Physiol Rev, 2005, 85: 1159-1204. |
[3] | Ma H, Yanofsky M F, Meyerowitz E M.Molecular cloning and characterization of GPA1, a G protein alpha subunit gene fromArabidopsis thaliana. Proc Natl Acad Sci USA, 1990, 87: 3821-3825. |
[4] | Ma H, Yanofsky M F, Hai H.Isolation and sequence analysis of TGA1 cDNAs encoding a tomato G protein α subunit.Gene, 1991, 107: 189-195. |
[5] | Misra S, Wu Y, Venkataraman G, et al.Heterotrimeric G-protein complex and G-protein-coupled receptor from a legume (Pisum sativum): Role in salinity and heat stress and cross-talk with phospholipase C.Plant J, 2007, 51: 656-669. |
[6] | Ishikawa A, Tsubouchi H, Iwasaki Y, et al.Molecular cloning and characterization of a cDNA for the α subunit of a G protein from rice.Plant Cell Physiol, 1995, 36: 353-359. |
[7] | Seo H S, Kim H Y, Jeong J Y, et al.Molecular cloning and characterization of RGA1 encoding a G protein α subunit from rice (Oryza sativa L. IR-36).Plant Mol Biol, 1995, 27: 1119-1131. |
[8] | Ashikari M, Wu J, Yano M, et al.Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the α-subunit of GTP-binding protein.Proc Natl Acad Sci USA, 1999, 96: 10284-10289. |
[9] | Mason M G, Botella J R.Isolation of a novel G-protein γ-subunit from Arabidopsis thaliana and its interaction with Gβ.Biochim Biophys Acta (BBA):Gene Struct Exp, 2001, 1520: 147-153. |
[10] | Weiss C A, Garnaat C W, Mukai K, et al.Isolation of cDNAs encoding guanine nucleotide-binding protein beta-subunit homologues from maize (ZGB1) and Arabidopsis (AGB1).Proc Natl Acad Sci USA, 1994, 91: 9554-9558. |
[11] | Ishikawa A, Iwasaki Y, Asahi T.Molecular cloning and characterization of a cDNA for the rβ subunit of a G protein from rice.Plant Cell Physiol, 1996, 37: 223-228. |
[12] | Kato C, Mizutani T, Tamaki H, et al.Characterization of heterotrimeric G protein complexes in rice plasma membrane.Plant J, 2004, 38: 320-331. |
[13] | Mason M G, Botella J R.Completing the heterotrimer: Isolation and characterization of an Arabidopsis thaliana G protein γ-subunit cDNA.Proc Natl Acad Sci USA, 2000, 97: 14784-14788. |
[14] | Urano D, Chen J G, Botella J R, et al.Heterotrimeric G protein signalling in the plant kingdom.Open Biol, 2013, 3: 120186. |
[15] | Yan S, Zou G, Li S, et al.Seed size is determined by the combinations of the genes controlling different seed characteristics in rice.Theor Appl Genet, 2011, 123: 1173-1181. |
[16] | Fan C, Yu S, Wang C, et al.A causal C-A mutation in the second exon of GS3 highly associated with rice grain length and validated as a functional marker.Theor Appl Genet, 2009, 118: 465-472. |
[17] | Takano-Kai N, Jiang H, Kubo T, et al.Evolutionary history of GS3, a gene conferring grain length in rice.Genetics, 2009, 182: 1323-1334. |
[18] | Wang C, Chen S, Yu S.Functional markers developed from multiple loci in GS3 for fine marker-assisted selection of grain length in rice.Theor Appl Genet, 2011, 122: 905-913. |
[19] | Takano-Kai N, Doi K, Yoshimura A.GS3 participates in stigma exsertion as well as seed length in rice.Breeding Sci, 2011, 61: 244-250. |
[20] | Mao H, Sun S, Yao J, et al.Linking differential domain functions of the GS3 protein to natural variation of grain size in rice.Proc Natl Acad Sci USA, 2010, 107: 19579-19584. |
[21] | Fan C, Xing Y, Mao H, et al.GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein.Thero Appl Genet, 2006, 112: 1164-1171. |
[22] | Sun H, Qian Q, Wu K, et al.Heterotrimeric G proteins regulate nitrogen-use efficiency in rice.Nat Genet, 2014, 46: 652-656. |
[23] | Yi X, Zhang Z, Zeng S, et al.Introgression of qPE9-1 allele, conferring the panicle erectness, leads to the decrease of grain yield per plant in japonica rice (Oryza sativa L.).J Genet Genom, 2011, 38: 217-223. |
[24] | Huang X, Qian Q, Liu Z, et al.Natural variation at the DEP1 locus enhances grain yield in rice.Nat Genet, 2009, 41: 494-497. |
[25] | Taguchi-Shiobara F, Kawagoe Y, Kato H, et al.A loss-of-function mutation of rice DENSE PANICLE 1 causes semi-dwarfness and slightly increased number of spikelets.Breeding Sci, 2011, 61: 17-25. |
[26] | Yan C-J, Zhou J-H, Yan S, et al.Identification and characterization of a major QTL responsible for erect panicle trait in japonica rice (Oryza sativa L.).Thero Appl Genet, 2007, 115: 1093-1100. |
[27] | Zhou Y, Zhu J, Li Z, et al.Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication.Genetics, 2009, 183: 315-324. |
[28] | Izawa Y, Takayanagi Y, Inaba N, et al.Function and expression pattern of the α subunit of the heterotrimeric G protein in rice.Plant Cell Physiol, 2010, 51: 271-281. |
[29] | Ueguchi-Tanaka M, Fujisawa Y, Kobayashi M, et al.Rice dwarf mutant d1, which is defective in the α subunit of the heterotrimeric G protein, affects gibberellin signal transduction.Proc Natl Acad Sci USA, 2000, 97: 11638-11643. |
[30] | Fujisawa Y, Kato T, Ohki S, et al.Suppression of the heterotrimeric G protein causes abnormal morphology, including dwarfism, in rice.Proc Natl Acad Sci USA, 1999, 96: 7575-7580. |
[31] | Assmann S M.G protein signaling in the regulation of rice seed germination.Sci STKE, 2015(310):12. |
[32] | Wang L, Xu Y Y, Ma Q B, et al.Heterotrimeric G protein α subunit is involved in rice brassinosteroid response.Cell Res, 2006, 16: 916-922. |
[33] | Oki K, Fujisawa Y, Kato H, et al.Study of the constitutively active form of the α subunit of rice heterotrimeric G proteins.Plant Cell Physiol, 2005, 46: 381-386. |
[34] | Yang G, Matsuoka M, Iwasaki Y, et al.A novel brassinolide-enhanced gene identified by cDNA microarray is involved in the growth of rice.Plant Mol Biol, 2003, 52: 843-854. |
[35] | Hu X, Qian Q, Xu T, et al.The U-box E3 ubiquitin ligase TUD1 functions with a heterotrimeric G α subunit to regulate brassinosteroid-mediated growth in rice.PLoS Genet, 2013, 9: e1003391. |
[36] | Lieberherr D, Thao N P, Nakashima A, et al.A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice.Plant Physiol, 2005, 138: 1644-1652. |
[37] | Suharsono U, Fujisawa Y, Kawasaki T, et al.The heterotrimeric G protein α subunit acts upstream of the small GTPase Rac in disease resistance of rice.Proc Natl Acad Sci USA, 2002, 99: 13307-13312. |
[38] | Assmann S M.G protein regulation of disease resistance during infection of rice with rice blast fungus.Sci STKE, 2005: cm13. |
[39] | Trusov Y, Rookes J E, Chakravorty D, et al.Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling.Plant Physiol, 2006, 140: 210-220. |
[40] | Lorbiecke R, Sauter M.Adventitious root growth and cell-cycle induction in deepwater rice.Plant Physiol, 1999, 119: 21-30. |
[41] | Steffens B, Sauter M.Epidermal cell death in rice is confined to cells with a distinct molecular identity and is mediated by ethylene and H2O2 through an autoamplified signal pathway.Plant Cell, 2009, 21: 184-196. |
[42] | Mergemann H, Sauter M.Ethylene induces epidermal cell death at the site of adventitious root emergence in rice.Plant Physiol, 2000, 124: 609-614. |
[43] | Joo J H, Wang S, Chen J, et al.Different signaling and cell death roles of heterotrimeric G protein α and β subunits in the Arabidopsis oxidative stress response to ozone.Plant Cell, 2005, 17: 957-970. |
[44] | Steffens B, Sauter M.G proteins as regulators in ethylene-mediated hypoxia signaling.Plant signal Behav, 2010, 5: 375-378. |
[45] | Steffens B, Sauter M.Heterotrimeric G protein signaling is required for epidermal cell death in rice.Plant Physiol, 2009, 151: 732-740. |
[46] | Utsunomiya Y, Samejima C, Takayanagi Y, et al.Suppression of the rice heterotrimeric G protein beta-subunit gene, RGB1, causes dwarfism and browning of internodes and lamina joint regions.Plant J, 2011, 67: 907-916. |
[47] | Utsunomiya Y, Samejima C, Fujisawa Y, et al.Rice transgenic plants with suppressed expression of the β subunit of the heterotrimeric G protein.Plant Signal Behav, 2012, 7: 443-446. |
[48] | Yadav D K, Shukla D, Tuteja N.Isolation, in silico characterization, localization and expression analysis of abiotic stress-responsive rice G-protein β subunit (RGB1).Plant Signal Behav, 2014, 9(5):e28890. |
[49] | Trusov Y, Chakravorty D, Botella J R.Diversity of heterotrimeric G-protein γ subunits in plants.BMC Res Notes, 2012, 5: 608. |
[50] | Chen J G, Gao Y, Jones A M.Differential roles of Arabidopsis heterotrimeric G-protein subunits in modulating cell division in roots.Plant Physiol, 2006, 141: 887-897. |
[51] | Choudhury S R, Bisht N C, Thompson R, et al.Conventional and novel Gγ protein families constitute the heterotrimeric G-protein signaling network in soybean.PLoS One, 2011, 6: e23361. |
[52] | Chakravorty D, Trusov Y, Zhang W, et al.An atypical heterotrimeric G-protein gamma-subunit is involved in guard cell K(+)-channel regulation and morphological development in Arabidopsis thaliana.Plant J, 2011, 67: 840-851. |
[53] | Yadav D K, Islam S M, Tuteja N.Rice heterotrimeric G-protein gamma subunits (RGG1 and RGG2) are differentially regulated under abiotic stress.Plant Signal Behav, 2012, 7: 733-740. |
[54] | Fan C, Xing Y, Mao H, et al.GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein.Thero Appl Genet, 2006, 112: 1164-1171. |
[55] | Li S, Liu Y, Zheng L, et al.The plant-specific G protein gamma subunit AGG3 influences organ size and shape in Arabidopsis thaliana.New Phytol, 2012, 194: 690-703. |
[56] | Jones A M, Assmann S M.Plants: The latest model system for G-protein research.EMBO Rep, 2004, 5: 572-578. |
[57] | Li S, Liu W, Zhang X, et al.Roles of the Arabidopsis G protein γ subunit AGG3 and its rice homologs GS3 and DEP1 in seed and organ size control.Plant Signal Behav, 2012, 7: 1357-1359. |
[58] | Botella J R.Can heterotrimeric G proteins help to feed the world?Trends Plant Sci, 2012, 17: 563-568. |
[59] | Trusov Y, Zhang W, Assmann S M, et al.Ggamma1 + Ggamma2 not equal to Gbeta: Heterotrimeric G protein Ggamma-deficient mutants do not recapitulate all phenotypes of Gbeta-deficient mutants.Plant Physiol, 2008, 147: 636-649. |
[60] | Li S J, Liu W X, Zhang X Q, et al.Roles of the Arabidopsis G protein γ subunit AGG3 and its rice homologs GS3 and DEP1 in seed and organ size control .Plant Signal Behav, 2012, 7(10): 1357-1359. |
[61] | Kunihiro S, Saito T, Matsuda T, et al.Rice DEP1, encoding a highly cysteine-rich G protein gamma subunit, confers cadmium tolerance on yeast cells and plants.J Exp Bot, 2013, 64: 4517-4527. |
[62] | Chen Y L, Huang R, Xiao Y M, et al.Extracellular calmodulin-induced stomatal closure is mediated by heterotrimeric G protein and H2O2.Plant Physiol, 2004, 136: 4096-4103. |
[63] | Coursol S, Fan L M, Le Stunff H, et al.Sphingolipid signalling in Arabidopsis guard cells involves heterotrimeric G proteins.Nature, 2003, 423: 651-654. |
[64] | Li J H, Liu Y Q, Lü P, et al.A signaling pathway linking nitric oxide production to heterotrimeric G protein and hydrogen peroxide regulates extracellular calmodulin induction of stomatal closure in Arabidopsis.Plant Physiol, 2009, 150: 114-124. |
[65] | Melotto M, Underwood W, He S Y.Role of stomata in plant innate immunity and foliar bacterial diseases.Annu Rev Phytopathol, 2008, 46: 101. |
[66] | Schroeder J I, Allen G J, Hugouvieux V, et al.Guard cell signal transduction.Annu Rev Phytopathol, 2001, 52: 627-658. |
[67] | Shimazaki K I, Doi M, Assmann S M, et al.Light regulation of stomatal movement.Annu Rev Plant Biol, 2007, 58: 219-247. |
[68] | Wang X Q, Ullah H, Jones A M, et al.G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells.Science, 2001, 292: 2070-2072. |
[69] | Zhang W, He S Y, Assmann S M.The plant innate immunity response in stomatal guard cells invokes G-protein-dependention channel regulation.Plant J, 2008, 56: 984-996. |
[70] | Sánchez-Rodríguez C, Estévez J M, Llorente F, et al.The ERECTA receptor-like kinase regulates cell wall-mediated resistance to pathogens in Arabidopsis thaliana.Mole Plant-microbe Interac, 2009, 22: 953-963. |
[71] | Gookin T E, Kim J, Assmann S M.Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: Computational prediction and in-vivo protein coupling.Genome Biol, 2008, 9: R120. |
[1] | 任志奇, 薛可欣, 董铮, 李小湘, 黎用朝, 郭玉静, 刘文强, 郭梁, 盛新年, 刘之熙, 潘孝武. 水稻外卷叶突变体ocl1的鉴定及基因定位[J]. 中国水稻科学, 2023, 37(4): 337-346. |
[2] | 肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A*/bar基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358. |
[3] | 李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367. |
[4] | 汪胜勇, 陈宇航, 陈会丽, 黄钰杰, 张啸天, 丁双成, 王宏伟. 水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响[J]. 中国水稻科学, 2023, 37(4): 368-378. |
[5] | 董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404. |
[6] | 韩聪, 何禹畅, 吴丽娟, 郏丽丽, 王磊, 鄂志国. 水稻碱性亮氨酸拉链(bZIP)蛋白家族功能研究进展[J]. 中国水稻科学, 2023, 37(4): 436-448. |
[7] | 沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232. |
[8] | 段敏, 谢留杰, 高秀莹, 唐海娟, 黄善军, 潘晓飚. 利用CRISPR/Cas9技术创制广亲和水稻温敏雄性不育系[J]. 中国水稻科学, 2023, 37(3): 233-243. |
[9] | 程玲, 黄福钢, 邱一埔, 王心怡, 舒宛, 邱永福, 李发活. 籼稻材料570011抗褐飞虱基因的遗传分析及鉴定[J]. 中国水稻科学, 2023, 37(3): 244-252. |
[10] | 王文婷, 马佳颖, 李光彦, 符卫蒙, 李沪波, 林洁, 陈婷婷, 奉保华, 陶龙兴, 符冠富, 秦叶波. 高温下不同施肥量对水稻产量品质形成的影响及其与能量代谢的关系分析[J]. 中国水稻科学, 2023, 37(3): 253-264. |
[11] | 刘嫒桦, 李小坤. 不同肥料施用与稻米品质关系的整合分析[J]. 中国水稻科学, 2023, 37(3): 276-284. |
[12] | 杨晓龙, 王彪, 汪本福, 张枝盛, 张作林, 杨蓝天, 程建平, 李阳. 不同水分管理方式对旱直播水稻产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 285-294. |
[13] | 魏晓东, 宋雪梅, 赵凌, 赵庆勇, 陈涛, 路凯, 朱镇, 黄胜东, 王才林, 张亚东. 硅锌肥及其施用方式对南粳46产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 295-306. |
[14] | 林聃, 江敏, 苗波, 郭萌, 石春林. 水稻高温热害模型研究及其在福建省的应用[J]. 中国水稻科学, 2023, 37(3): 307-320. |
[15] | 郑承梅, 孙金秋, 刘梦杰, 杨永杰, 陆永良, 郭怡卿, 唐伟. 水稻田糠稷种子萌发和出苗特性及化学防除药剂筛选[J]. 中国水稻科学, 2023, 37(3): 321-328. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||