中国水稻科学 ›› 2011, Vol. 25 ›› Issue (5): 455-460.DOI: 10.3969/j.issn.10017216.2011.05.001

• 研究报告 •    下一篇

一个水稻卷叶基因rl(t)的精细定位

潘存红1, 2, #,李磊1,#,陈宗祥1,薛芗1,张亚芳1,左示敏1,戴正元2 ,潘学彪1,*,马玉银1,3,*   

  1. 1 扬州大学 江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室,  江苏 扬州  225009; 2 江苏里下河地区农业科学研究所/国家水稻改良中心南京分中心,  江苏 扬州 225007; 3扬州职业大学 生化工程系, 江苏 扬州225009;
  • 收稿日期:2010-10-26 修回日期:2010-11-25 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 潘学彪1,*马玉银1,3,*
  • 基金资助:

     江苏省自然科学基金资助项目(BK2008223,BK2009696);  江苏高校省级重点实验室开放课题资助项目(K09004)

Fine Mapping of a Rolled Leaf Gene rl(t)    in Rice

PAN Cun-hong 1, 2, #,LI Lei 1, #, CHEN Zong-xiang 1, XUE Xiang 1,  ZHANG Ya-fang 1, ZUO Shi-min 1,  DAI Zheng-yuan 2, PAN Xue-biao 1,* ,   MA Yu-yin 1, 3,*   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China; 2 Lixiahe Agricultural Research Institute of Jiangsu Province/Nanjing Subcenter of National Rice Improvement Center, Yangzhou 225007, China; 3 Biochemical Engineering Department, Yangzhou Polytechnic College, Yangzhou 225009, China;
  • Received:2010-10-26 Revised:2010-11-25 Online:2011-09-10 Published:2011-09-10
  • Contact: PAN Xue-biao1,*, MA Yu-yin1, 3,*

摘要: 叶片适度卷曲是水稻理想株型塑造的重要组成部分。卷叶基因rl(t)在杂合状态下可使叶片适度卷曲,增加水稻产量,具有较高的育种利用价值。以卷叶珍汕97B\[携带rl(t)基因\]与平展叶品种奇妙香(轮回亲本)杂交并回交的BC5F3和BC5F4为定位群体,在前人关于rl(t)的定位区间内设计了15对多态性分子标记,将rl(t)精细定位于第2染色体上分子标记P95053和P113.6之间的11 kb范围内,遗传间距约0.04 cM。区间内只包含1个释义基因,预测编码GL2类同源异型结构域,属于同源异型盒家族中的HDGL2类(也称为HDZIP Ⅳ)转录因子。卷叶基因rl(t)的精细定位为克隆目标基因和研究其调控机理奠定了基础。

关键词: 水稻, 卷叶基因, 精细定位

Abstract: Moderately  rolled  leaf   is  one of the  most important morphological characters in ideal plant architecture breeding in rice. The heterozygous   rl(t) gene   could produce moderately rolled leaf and   increase rice yield, presenting a high breeding potential.  By using  BC5F3  and BC5F4 populations derived from   a rolled  leaf Zhenshan 97B(carrying rl(t) gene) and  a flatleaf  variety Qimiaoxiang(recurrent parent),  and a total of 15 polymorphic markers, the rl(t) gene was ultimately mapped into a region of 11 kb flanked by the markers P95053 and P113.6  on chromosome 2. The genetic  distance of  this region is about 0.04 cM. Only one predicated ORF (open reading frame)/gene was  found in this region, which encodes  a transcription factor of  the homeobox family HDGL2 (also known as HDZIP Ⅳ). The results laid a   foundation for further cloning  of rl(t)and understanding of  its regulation mechanism.

Key words: rice, rolled leaf gene, fine mapping

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