一个水稻苗期白条纹叶及抽穗期白穗突变体的鉴定和基因定位

doi:10.3969/j.issn.10017216.2011.05.002

中国水稻科学 ›› 2011, Vol. 25 ›› Issue (5): 461-466.DOI: 10.3969/j.issn.10017216.2011.05.002

一个水稻苗期白条纹叶及抽穗期白穗突变体的鉴定和基因定位

金怡^1,2,刘合芹¹,汪得凯^1,*,陶跃之^1,*

¹ 浙江省农业科学院作物与核技术利用研究所，浙江杭州 310021;² 浙江师范大学化学与生命科学学院，浙江金华 321004;

收稿日期:2010-12-30 修回日期:2011-01-19 出版日期:2011-09-10 发布日期:2011-09-10
通讯作者: 汪得凯1,*,陶跃之1,*
基金资助:
转基因生物新品种培育科技重大专项（2008ZX08010002）；浙江省自然科学基金重点项目（Z3110509）。

Genetic Analysis and Gene Mapping of a White Striped Leaf and White Panicle Mutant in Rice

JIN Yi ^1,2 , LIU Heqin ¹ ,WANG Dekai^1，* ,TAO Yuezhi ^1,*

¹ Institute of Crop and Nuclear Technology Utilization， Zhejiang Academy of Agricultural Sciences, Hangzhou 310021， China; ²College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;

Received:2010-12-30 Revised:2011-01-19 Online:2011-09-10 Published:2011-09-10
Contact: WANG Dekai1，*, TAO Yuezhi1,*

摘要/Abstract

摘要： 从粳稻中花11组培后代中发现了一个苗期白条纹，抽穗期白穗的突变体。该突变体表现为1叶期叶全白，2叶期从新叶叶尖开始沿叶脉逐渐转绿，至成株期完全变绿，抽穗后内外颖表现为白色，穗轴和小枝梗表现为绿色，成熟后颖壳转黄。根据基因定位结果，将该突变体定名为wslwp(white striped leaf and white panicle)。与野生型相比，wslwp突变体2叶期及抽穗期叶片的叶绿素含量、类胡萝卜素含量及结实率均显著降低。遗传分析表明，该突变表型受1对隐性核基因控制，非TDNA插入引起。为了克隆WSLWP基因，利用wslwp突变体与籼稻品种龙特甫B杂交获得的F2分离群体进行基因定位，首先将该基因定位于水稻第7染色体上的SSR标记RM5711与RM6574之间。随后，利用已有的SSR标记和开发的STS标记，进一步将该基因定位在STS763和STS765之间，物理距离约为87 kb。

关键词: 水稻font-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">, 白条纹叶及白穗突变体font-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">; 微卫星标记font-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">;基因定位

Abstract: A white striped leaf and white panicle mutant, designated as wslwp, was obtained from a rice (Oryza sativa L. ssp. japonica cv. Zhonghua 11) transfer DNA insertion mutant pool. The mutant was characterized by white leaves at the 1leaf stage, chlorotic leaves with longitudinal green stripes at the 2leaf stage, and green leaves before heading. Moreover, at the heading stage, the glume showed white color, but the panicle axis and rachis remained green. The grain changed into yellow at the maturity stage. Chlorophyll and carotenoid contents and seed setting rate of the mutant were significantly reduced. Genetic analysis showed that the phenotype of the mutnt was controlled by a single recessive nuclear gene. An F2 population generated by crossing the mutant with Longtefu B was used as a gene mapping population. WSLWP locus was preliminarily located between SSR molecular markers RM5711 and RM6574 on chromosome 7, then it was mapped to a physical distance 87 kb region between newlydeveloped polymorphic STS markers STS763 and STS765.

Key words: ricefont-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">;white striped leaf , and white panicle , mutantfont-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">;simple sequence repeat , markerfont-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">;gene mapping

中图分类号:

金怡1,2,刘合芹1,汪得凯1,*,陶跃之1,*. 一个水稻苗期白条纹叶及抽穗期白穗突变体的鉴定和基因定位[J]. 中国水稻科学, 2011, 25(5): 461-466.

JIN Yi1,2 , LIU Heqin1 , WANG Dekai1，*,TAO Yuezhi1,*. Genetic Analysis and Gene Mapping of a White Striped Leaf and White Panicle Mutant in Rice[J]. Chinese Journal of Rice Science, 2011, 25(5): 461-466.

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一个水稻苗期白条纹叶及抽穗期白穗突变体的鉴定和基因定位

Genetic Analysis and Gene Mapping of a White Striped Leaf and White Panicle Mutant in Rice

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