孕穗期水稻不同功能叶的发育蛋白质组学分析

doi:10.3969/j.issn.10017216.2009.05.02

摘要/Abstract

摘要： 从蛋白质组学角度对杂交水稻汕优63孕穗期4个叶位功能叶片蛋白质组变化进行了研究，以揭示水稻孕穗期叶片蛋白质组的表达差异。4个不同时期功能叶片蛋白质经双向电泳得到分离，应用Imagemaster 2D Elite 5.0软件对所得到的双向电泳图谱进行分析比较，共得到差异表达蛋白质点23个，差异表达蛋白质点经质谱分析，有14个得到鉴定。对鉴定出的蛋白功能进行研究，结果表明:差异表达的蛋白质大部分(9个)为参与光合作用和呼吸作用的蛋白质；其次为与蛋白结构及功能调控相关的蛋白质(3个)，表达量表现为随叶位上升而上调；其余为参与氨基酸代谢的蛋白质(2个)，表达量在较低叶位叶片中无显著规律，在剑叶中的表达量显著上调。蛋白质是细胞功能执行者，受细胞功能或细胞环境变化的影响，因此,上述蛋白质发生变化的原因应与水稻不同叶位叶片生长发育特性有关。

关键词: 水稻, 孕穗期, 叶位, 蛋白质组学, 差异蛋白质组

Abstract: n order to reveal proteomic changes in rice functional leaves collected from four different leaf positions, the proteins expression profile of functional leaves at the booting stage in hybrid rice Shanyou 63 were studied by using the approach of plant proteomics. Protein expression profiles of the top leaves at the 13,14,15 and 16leaf stages(the total number of leaves of Shanyou 63 was 16) were investigated by twodimensional gel electrophoresis. Differential protein expression among the four stages were analyzed by Imagemaster 2D Elite 5.0, which allowed the identification of 23 significantly different gel spots. The spots were further verified by matrixassisted laser desorption/ionizationtime of flight mass spectrometry, in which 14 were identified. The functions of these 14 proteins were analyzed and the conclusion can be drawn from the following result: the major differential expression proteins (9 proteins) were associated with photosynthesis and respiration, three differential expression proteins were related to protein formation and protein function control, and the expression abundance of those 12 proteins increased regularly with the leaf position elevation; the other proteins(2 proteins) were involved in amino acid metabolism, showing an irregularly changing pattern in lower leaf positions, and a distinctly increasing expression abundance in flag leaf. The protein is the carrier of cell function and significantly influenced by the variation of cell function or intercellular environment, therefore, the reason that caused the above protein changes should be related to the development of leaves at different leaf positions.

Key words: rice, booting stage, leaf position, proteomics, differential expression proteome

邵彩虹谢金水,黄永兰,邱才飞,张振苗,林文雄,. 孕穗期水稻不同功能叶的发育蛋白质组学分析[J]. 中国水稻科学 2009,23(5): 456-462 . , DOI: 10.3969/j.issn.10017216.2009.05.02 .

SHAO Cai-hong ,XIE Jin-shui ,HUANG Yong-lan ,QIU Cai-fei,ZHANG Zhen-miao,LIN Wen-xiong. Developmental Proteomics Analysis of Different Functional Leaves in Rice (Oryza sativa) at the Booting Stage [J]. Chinese Journal of Rice Science 2009,23(5): 456-462 ., DOI: 10.3969/j.issn.10017216.2009.05.02 .

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