中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻小粒矮突变性状与T-DNA插入标签的共分离检测

马玉银1, 3,张亚芳1,潘存红1,李爱宏1,吴旭江1,左示敏1,陈宗祥1,吴昌银2,潘学彪1,*   

  1. 1扬州大学 江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室, 江苏 扬州 225009; 2华中农业大学 作物遗传改良国家重点实验室, 湖北 武汉 430070; 3扬州教育学院 生物化学系, 江苏 扬州 225002; *通讯联系人, E-ail: shuidao@yzu.edu.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-11-10 发布日期:2008-11-10

Co-segregation Analysis Between TDNA Tag and Character of Small Grain Dwarf Mutant in Rice

MA Yuyin1,3, ZHANG Yafang1, PAN Cunhong1, LI Aihong1, WU Xujiang1, ZUO Shimin1, CHEN Zongxiang1, WU Changyin2, PAN Xuebiao1,*   

  1. 1Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genetics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; 2National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 3Department of Biochemistry, Yangzhou College of Education, Yangzhou 225002, China; *Corresponding author, E-ail: shuidao@yzu.edu.cn
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-10 Published:2008-11-10

摘要: 从T-DNA水稻标签系中发现1个标签系中出现了小粒矮(sgd)突变性状的分离。为克隆该突变基因和进行基因功能研究,对该突变体进行了遗传分析。该标签系存在多个插入事件,且T3代发现目标突变性状分离系中,另一个非目标插入事件已经纯合,故特异PCR检测不能确认小粒矮突变性状与T-NA插入共分离。但通过TAIL-CR方法,获得了插入点侧翼的水稻基因组序列,并证实该侧翼序列与小粒矮突变性状共分离。

关键词: T-NA标签技术, 侧翼序列, 共分离, 水稻, 小粒矮突变

Abstract: A small grain dwarf (sgd) mutant was identified in the segregated population from a TDNAtag rice line. Genetic analysis of this mutant was conducted for cloning and functional research of the gene. Multiple TDNA insertion copies in this line were confirmed, and one of them had been homozygous in T3 segregation population. As a result, it was impossible to detect cosegregation between the tag and sgd merely depended on the specific PCR primer from TDNA in T3 and successively generations. However, a flanking sequence from one of TDNA insertion sites was obtained by TAILPCR, and was confirmed to be cosegregated with sgd in consecutively segregated generations.

Key words: T-NA tagging technology, flanking sequence, cosegregation, rice, small grain dwarf mutant